scholarly journals Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis

2019 ◽  
Vol 13 (1) ◽  
pp. 481-488 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Peter Kollár
Keyword(s):  
Ulcerative Colitis ◽  
Bowel Disease ◽  
Principal Component ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Reducing Bacteria

AbstractIntestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction inDesulfovibriospecies that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations,p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R2) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H2S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.

scholarly journals Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Open Medicine ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Monika Vítězová
Keyword(s):  
Ulcerative Colitis ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Alkaline Media ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

AbstractLower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (<5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (>9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

scholarly journals Metabolic activity of sulfate-reducing bacteria from rodents with colitis

Open Medicine ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 344-349 ◽  
Author(s):  
Jozef Kováč ◽  
Monika Vítězová ◽  
Ivan Kushkevych
Keyword(s):  
Ulcerative Colitis ◽  
Large Intestine ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Human ◽  
Sulfate Reducing ◽  
Anaerobic Microorganisms ◽  
The Difference ◽  
High Concentrations ◽  
Reducing Bacteria

AbstractSulfate-reducing bacteria (SRB) are anaerobic microorganisms, which use sulfate as an electron acceptor in the process of dissimilatory sulfate reduction. The final metabolic product of these anaerobic microorganisms is hydrogen sulfide, which is known as toxic and can lead to damage to epithelial cells of the large intestine at high concentrations. Different genera of SRB are detected in the large intestine of healthy human and animals, and with diseases like Crohn’s disease and ulcerative colitis. SRB isolated from rodents with ulcerative colitis have produced 1.14 (mice) and 1.03 (rats) times more sulfide ions than healthy rodents. The species ofDesulfovibriogenus are the most widespread among all SRB in the intestine. The object of our research was to observe and compare the difference of production of sulfide and reduction of sulfate in intestinal SRB isolated from healthy rodents and rodents with ulcerative colitis.

Removal of lead by sulfate-reducing bacteria in anaerobic continuous stirred tank reactors

2016 ◽  
Vol 14 (3) ◽  
pp. 557-561
Author(s):  
Nguyễn Thị Yên ◽  
Kiều Thị Quỳnh Hoa
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Stirred Tank ◽  
Synthetic Wastewater ◽  
Terminal Electron Acceptor ◽  
Stirred Tank Reactors ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Continuous Stirred Tank ◽  
Continuous Stirred Tank Reactors ◽  
Reducing Bacteria

Lead contaminated wastewater negatively impacts to living organisms as well as humans. In recent years, a highly promising biological process using the anaerobic production of sulfide ions by sulfate-reducing bacteria has presented itself as an alternative option for the removal of lead. This process is based on microbial utilization of electron donors, such as organic compounds (carbon sources), and sulfate as the terminal electron acceptor for sulfide production. The biogenic hydrogen sulfide reacts with dissolved heavy metals to form insoluble metal sulfide precipitates Removal of lead by an enriched consortium of sulfate-reducing bacteria (DM10) was evaluated sulfate reduction, sulfide production and lead precipitation. Four parallel anaerobic continuous stirred tank reactors (CSTR, V = 2L) (referred as R1 - R4) were fed with synthetic wastewater containing Pb2+ in the concentrations of 0, 100, 150 and 200 mg L-1 of lead and operated with a hydraulic retention time of 5 days for 40 days. The loading rates of each metal in R1- R4 were 0, 20, 30 and 40 mg L-1 d-1, respectively. The results showed that there was no inhibition of SRB growth and that lead removal efficiencies of 99-100% for Pb2+ were achieved in R2 (100 mg L-1) and R3 (150 mg L-1) throughout the experiment. For the highest lead concentration of  200 mg L-1, a decrease in efficiency of removal (from 100 to 96%) was observed at the end of the experiment. The obtained result of this study might help for a better control operation and performance improvements of reactors.

Optimization of sulfide production by an indigenous consortium of sulfate-reducing bacteria for the treatment of lead-contaminated wastewater

2015 ◽  
Vol 38 (10) ◽  
pp. 2003-2011 ◽  
Author(s):  
Thi Quynh Hoa Kieu ◽  
Thi Yen Nguyen ◽  
Thi Yen Dang ◽  
Thanh Binh Nguyen ◽  
Thi Nga Vuong ◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Reducing Bacteria

scholarly journals Sulfidogenic activity of bacteria Desulfomicrobium sp. CrR3 under the influence of nitrite and sodium molybdate

2016 ◽  
pp. 131-135
Author(s):  
L. Dorosh ◽  
T. Peretiatko ◽  
S. Gudz
Keyword(s):  
Hydrogen Sulfide ◽  
Sodium Molybdate ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Complete Inhibition ◽  
Simultaneous Action ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
The City

Sulfate reducing bacteria Desulfomicrobium sp. CrR3, selected from the wastewater of the city, do not accumulate biomass under the influence of nitrite and molybdate ions. These substances inhibit sulfidogenic activity of bacteria Desulfomicrobium sp. CrR3. Additon of nitrite at a concentration of 1 mM has virtually no effect on the accumulation of biomass and hydrogen sulfide by bacteria Desulfomicrobium sp. CrR3. The increasing the concentration of nitrite up to 5 mM biomass accumulation of bacteria Desulfomicrobium sp. CrR3 was inhibited by approximately 50% while the observed decrease in efficiency of sulfate compared  with control. Molybdate at a concentration of 0.5–1 mM completely inhibits the growth and sulfidogenic activity of Desulfomicrobium sp. CrR3. Simultaneous action of nitrite and molybdate causes almost complete inhibition of Desulfomicrobium sp. CrR3 growth. Under these conditions the bacteria do not reduced sulfate and as a result do not accumulate hydrogen sulfide.

scholarly journals Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria

2005 ◽  
Vol 71 (8) ◽  
pp. 4610-4618 ◽  
Author(s):  
Naghma Naz ◽  
Hilary K. Young ◽  
Nuzhat Ahmed ◽  
Geoffrey M. Gadd
Keyword(s):  
Heavy Metal ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Cadmium Accumulation ◽  
Metal Resistance ◽  
Dot Blot ◽  
Cadmium Resistance ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Reducing Bacteria

ABSTRACT Cadmium resistance (0.1 to 1.0 mM) was studied in four pure and one mixed culture of sulfate-reducing bacteria (SRB). The growth of the bacteria was monitored with respect to carbon source (lactate) oxidation and sulfate reduction in the presence of various concentrations of cadmium chloride. Two strains Desulfovibrio desulfuricans DSM 1926 and Desulfococcus multivorans DSM 2059 showed the highest resistance to cadmium (0.5 mM). Transmission electron microscopy of the two strains showed intracellular and periplasmic accumulation of cadmium. Dot blot DNA hybridization using the probes for the smtAB, cadAC, and cadD genes indicated the presence of similar genetic determinants of heavy metal resistance in the SRB tested. DNA sequencing of the amplified DNA showed strong nucleotide homology in all the SRB strains with the known smtAB genes encoding synechococcal metallothioneins. Protein homology with the known heavy metal-translocating ATPases was also detected in the cloned amplified DNA of Desulfomicrobium norvegicum I1 and Desulfovibrio desulfuricans DSM 1926, suggesting the presence of multiple genetic mechanisms of metal resistance in the two strains.

scholarly journals Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria

2021 ◽  
Vol 11 (3) ◽  
pp. 1152
Author(s):  
Ivan Kushkevych ◽  
Daryna Abdulina ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Monika Vítězová ◽  
...  
Keyword(s):  
Trace Elements ◽  
Trace Metals ◽  
Inductively Coupled Plasma ◽  
Chemical Elements ◽  
Principal Component ◽  
Sulfate Reducing Bacteria ◽  
Bacterial Cells ◽  
Sulfate Reducing ◽  
Total Variability ◽  
Reducing Bacteria

The monitoring of trace metals in microbial cells is relevant for diagnosis of inflammatory bowel disease (IBD). Sulfate-reducing bacteria (SRB) represent an important factor in the IBD development. The content of trace metals in bacterial cells may reflect the functioning of the enzyme systems and the environmental impact on the occurrence of SRB. The aim of our research was to compare the content of trace elements in the cells of SRB cultures isolated from fecal samples of patients with IBD and healthy people. The contents of 11 chemical elements in the bacterial cells of SRB were analyzed by the inductively coupled plasma-mass-spectrometry (ICP-MS) method. Significant changes in the content of calcium, zinc, magnesium, potassium, and iron were observed in patients with IBD compared to healthy individuals. Through a principal component analysis (PCA), a total variability of 67.3% in the difference between the samples was explained. The main factors influencing the total variability in the bacterial cells of SRB isolated from patients suffering from IBD were the content of the micro- and trace elements, such as manganese (with power 0.87), magnesium and cobalt (0.86), calcium (0.84), molybdenum (0.81), and iron (0.78). Such changes in the elemental composition of SRB under different conditions of existence in the host may indicate adaptive responses of the microorganisms, including the inclusion of oxidative stress systems, which can lead to changes in SRB metabolism and the manifestation of parameters of IBD in humans. The use of PCA might make it possible in the future to predict the development and ratio of SRB in patients with various diseases.

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