Histological evaluation of socket preservation with different bone grafting materials

2014 ◽  
Vol 34 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Eisner Salamanca ◽  
Hsi-Kuei Lin ◽  
Sheng-Wei Feng ◽  
Haw-Ming Huang ◽  
Nai-Chia Teng ◽  
...  
Keyword(s):  
Bone Grafting ◽  
Control Site ◽  
Treatment Modalities ◽  
Histological Evaluation ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Socket Preservation ◽  
Bone Grafting Materials ◽  
Grafting Materials ◽  
Empty Control

Abstract The aim of this study was to compare different bone grafting materials and their contribution to socket preservation. In six beagle dogs, bilateral mandibular second, third, and fourth premolars were hemisectioned and extracted. The following four treatment modalities were randomly allocated: (1) empty control site, (2) collagen plug, (3) Bio Oss-collagen, (4) HA/β-TCP-collagen. After 2 and 4 weeks, the animals were sacrificed, and decalcified sections were obtained for histological analysis. New bone formation was homogeneous, progressive, and centripetal in all materials. Remodeling as well as the calcification of the newly formed bone was observed. In addition, well-connected trabeculae surrounding the degraded materials were observed. Nearly mature bone, which demonstrated less cellular, more mineralized, and structurally more organized into lamellar bone was noted in HA/β-TCP-collagen composite. In this study, Bio Oss-collagen and HA/β-TCP-collagen produced a newly biomimetic composite biomaterial and could prove to be a superior bone substitute material, both structurally and functionally, which could be used in the repair of bone defects collagen composite.

scholarly journals Effect of Different Bone Grafting Materials and Mesenchymal Stem Cells on Bone Regeneration: A Micro-Computed Tomography and Histomorphometric Study in a Rabbit Calvarial Defect Model

2021 ◽  
Vol 22 (15) ◽  
pp. 8101
Author(s):  
Shiau-Ting Shiu ◽  
Wei-Fang Lee ◽  
Sheng-Min Chen ◽  
Liu-Ting Hao ◽  
Yuan-Ting Hung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Autogenous Bone ◽  
New Bone Formation ◽  
Micro Computed Tomography ◽  
Defect Model ◽  
Bone Grafting Materials ◽  
Grafting Materials ◽  
Empty Control

This study evaluated the new bone formation potential of micro–macro biphasic calcium phosphate (MBCP) and Bio-Oss grafting materials with and without dental pulp-derived mesenchymal stem cells (DPSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) in a rabbit calvarial bone defect model. The surface structure of the grafting materials was evaluated using a scanning electron microscope (SEM). The multipotent differentiation characteristics of the DPSCs and BMSCs were assessed. Four circular bone defects were created in the calvarium of 24 rabbits and randomly allocated to eight experimental groups: empty control, MBCP, MBCP+DPSCs, MBCP+BMSCs, Bio-Oss+DPSCs, Bio-Oss+BMSCs, and autogenous bone. A three-dimensional analysis of the new bone formation was performed using micro-computed tomography (micro-CT) and a histological study after 2, 4, and 8 weeks of healing. Homogenously porous structures were observed in both grafting materials. The BMSCs revealed higher osteogenic differentiation capacities, whereas the DPSCs exhibited higher colony-forming units. The micro-CT and histological analysis findings for the new bone formation were consistent. In general, the empty control showed the lowest bone regeneration capacity throughout the experimental period. By contrast, the percentage of new bone formation was the highest in the autogenous bone group after 2 (39.4% ± 4.7%) and 4 weeks (49.7% ± 1.5%) of healing (p < 0.05). MBCP and Bio-Oss could provide osteoconductive support and prevent the collapse of the defect space for new bone formation. In addition, more osteoblastic cells lining the surface of the newly formed bone and bone grafting materials were observed after incorporating the DPSCs and BMSCs. After 8 weeks of healing, the autogenous bone group (54.9% ± 6.1%) showed a higher percentage of new bone formation than the empty control (35.3% ± 0.5%), MBCP (38.3% ± 6.0%), MBCP+DPSC (39.8% ± 5.7%), Bio-Oss (41.3% ± 3.5%), and Bio-Oss+DPSC (42.1% ± 2.7%) groups. Nevertheless, the percentage of new bone formation did not significantly differ between the MBCP+BMSC (47.2% ± 8.3%) and Bio-Oss+BMSC (51.2% ± 9.9%) groups and the autogenous bone group. Our study results demonstrated that autogenous bone is the gold standard. Both the DPSCs and BMSCs enhanced the osteoconductive capacities of MBCP and Bio-Oss. In addition, the efficiency of the BMSCs combined with MBCP and Bio-Oss was comparable to that of the autogenous bone after 8 weeks of healing. These findings provide effective strategies for the improvement of biomaterials and MSC-based bone tissue regeneration.

Maxillofacial Bone Grafting Materials

2021 ◽  
Vol 65 (1) ◽  
pp. 167-195
Author(s):  
Nabil Moussa ◽  
Yijiao Fan ◽  
Harry Dym
Keyword(s):  
Bone Grafting ◽  
Bone Grafting Materials ◽  
Grafting Materials

scholarly journals Characterization and angiogenic potential of xenogeneic bone grafting materials: Role of periodontal ligament cells

2016 ◽  
Vol 35 (6) ◽  
pp. 900-907 ◽  
Author(s):  
Charlotte ROMBOUTS ◽  
Charlotte JEANNEAU ◽  
Josette CAMILLERI ◽  
Patrick LAURENT ◽  
Imad ABOUT
Keyword(s):  
Bone Grafting ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Angiogenic Potential ◽  
Bone Grafting Materials ◽  
Grafting Materials

scholarly journals Spontaneous Bone Healing after Cysts Enucleation without Bone Grafting Materials: A Randomized Clinical Study

2015 ◽  
Vol 8 (1) ◽  
pp. 14-22 ◽  
Author(s):  
EduardoDaniel Rubio ◽  
CarlosMariano Mombrú
Keyword(s):  
Clinical Study ◽  
Bone Density ◽  
Bone Regeneration ◽  
Bone Grafting ◽  
Jaw Cysts ◽  
Randomized Clinical Study ◽  
Bone Grafting Materials ◽  
Left And Right ◽  
Grafting Materials ◽  
Total Average

The aim of this study was to evaluate spontaneous bone regeneration after cysts enucleation of the jaws without the use of bone grafting materials. We included 18 patients at random (11 men and 7 women) with a mean age of 31.8 years, with jaw cysts treated by enucleation, without the use of grafting materials. A method of measurements to assess the percentage of reduction of the bone cavities was used to objectify the results. The patients were evaluated before and at least 6 months after surgery, with radiographic scans based on linear measures with a computerized method using Nemoceph program (Nemotec, NemoCeph Software, Madrid, España). The analysis of the sample shows an average of 85.59% decrease in horizontal measures, 89.53% in the vertical, and 88.98 and 89.81% in the diagonal left and right, respectively. The total average reduction was 88.47%. It showed a greater decrease in vertical and diagonal measurements with respect to horizontal. Regeneration in 12 patients was 100% and in 6 patients was higher at 50.4%. Bone density increased in the postoperative radiographs. The results suggest that in some cases, spontaneous bone regeneration can be achieved by cysts enucleation without bone grafting materials.

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