Effect of Lignin-Containing Media on Growth of Medicinal Mushroom Lentinula Edodes

Abstract The effect of lignocellulose and lignin on growth of mycelium of mushroom Lentinula edodes and laccase activity in cultivation medium was studied. It was shown that cultivation of L. edodes mycelium in liquid nutrient medium with addition of 0.25-0.5% of kraft lignin increased mycelium biomass yield approximately two times compared with reference conditions without addition of lignin. Similar results were obtained in experiments in which 0.5% lignocellulose that remained after obtaining furfural, and 0.5% lignin that remained after obtaining furfural and glucose from wheat straw, were added to the nutrient medium. This effect was greater in the conditions of cultivation with good aeration, compared with static culture. Laccase activity in medium increased after addition of wheat straw lignocellulose or lignin only in the case of mycelium cultivation with aeration. In the case of mushroom cultivation on solid nutrient medium, addition of wheat straw lignocellulose and lignin promoted growth of mycelium only during the first 7 days of cultivation.

Download Full-text

Preference of fresh and stored Ceriporiopsis subvermispora and Lentinula edodes treated wheat straw by goats

Livestock Science ◽

10.1016/j.livsci.2020.104013 ◽

2020 ◽

Vol 236 ◽

pp. 104013

Author(s):

Lei Mao ◽

J. Leon M. Marchal ◽

Anton S.M. Sonnenberg ◽

John W. Cone ◽

Viviane Endo Hidalgo ◽

...

Keyword(s):

Wheat Straw ◽

Lentinula Edodes ◽

Ceriporiopsis Subvermispora

Download Full-text

Biomass yield and feeding value of rye, triticale, and wheat straw produced under a dual-purpose management system

Journal of Animal Science ◽

10.2527/jas2017.1888 ◽

2017 ◽

Vol 95 (11) ◽

pp. 4893 ◽

Cited By ~ 3

Author(s):

S. Ates ◽

G. Keles ◽

U. Demirci ◽

S. Dogan ◽

H. Ben Salem

Keyword(s):

Wheat Straw ◽

Management System ◽

Biomass Yield ◽

Dual Purpose ◽

Feeding Value

Download Full-text

Development of the technology for preparation of enzymatic hydrolysate of waste chick embryos

BIOpreparations Prevention Diagnosis Treatment ◽

10.30895/2221-996x-2021-21-3-200-205 ◽

2021 ◽

Vol 21 (3) ◽

pp. 200-205

Author(s):

Yu. S. Ovsyannikov ◽

M. S. Dursenev

Keyword(s):

Nutrient Medium ◽

Mass Fraction ◽

Chemical Properties ◽

Chick Embryos ◽

Nutrient Media ◽

Enzymatic Hydrolysate ◽

E Coli ◽

Physico Chemical ◽

Growth Properties ◽

Solid Nutrient Medium

The development of technologies for preparation of protein nutritional bases for microbiological nutrient media, from production waste of mainly readily available or non-food products, is a promising area in biotechnology. Researchers of Vyatka State Agrotechnological University assume that non-food secondary raw materials, such as waste chick embryos (WCEs) used in the production of anti-influenza products, could be used for these purposes, after removal of the virus-containing allantoic fluid. The aim of the study was to develop a technology for preparation of WCE enzymatic hydrolysate (WCEEH), and to evaluate growth properties of the hydrolysate-based solid nutrient medium, using Escherichia coli M-17 and Pseudomonas alcaligenes IP-1 test strains. Materials and methods: the authors offer methodological approaches to obtaining WCEEH and substantiate hydrolysis parameters. The obtained WCEEH was characterised in terms of physico-chemical properties: pH, amine nitrogen, total nitrogen, sodium chloride, degree of protein cleavage. The growth properties of the hydrolysate-based nutrient medium were studied using E. coli M-17 and Ps. alcaligenes IP-1 test strains. Results: the experiments demonstrated the feasibility of performing enzymatic hydrolysis of WCEs, and assessed physico-chemical properties of the prepared WCEEH batches. The study demonstrated the possibility of using the prepared hydrolysate as a component of solid nutrient media for growing the selected test strains. Conclusions: the study substantiated the optimal technological parameters for WCE enzymatic hydrolysis: pH (7.6 ± 0.3), duration (48 ± 2 h), temperature (49 ± 1) °C. The loading of hydrolysis components was optimised: mass fraction of the substrate—500 g/L, mass fraction of the hydrolysing agent—100 g/L. The physico-chemical properties of WCEEH make it suitable for preparation of microbiological media; the hydrolysate-based solid nutrient medium consistently ensures the growth of E. coli M-17 and Ps. alcaligenes IP-1 test strains with standard properties. The growth properties of the experimental medium are comparable to those of the meat-peptone broth-based nutrient medium.

Download Full-text

Analysis of the Effect of Media Types and Chromagenic Chemicals on the Detection of Extracellular Laccase Activity among Lentinula edodes Strains

The Korean Journal of Mycology ◽

10.4489/kjm.2011.39.1.048 ◽

2011 ◽

Vol 39 (1) ◽

pp. 48-52

Author(s):

Jun-Young Kim ◽

Hyuk-Woo Kwon ◽

Longqing Tang ◽

Han-Kyu Ko ◽

Seong-Hwan Kim

Keyword(s):

Laccase Activity ◽

Lentinula Edodes ◽

Extracellular Laccase ◽

Media Types

Download Full-text

Improvement of yield of the edible and medicinal mushroom Lentinula edodes on wheat straw by use of supplemented spawn

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822014000200013 ◽

2014 ◽

Vol 45 (2) ◽

pp. 467-474 ◽

Cited By ~ 7

Author(s):

Rigoberto Gaitán-Hernández ◽

Gerardo Mata Norberto Cortés

Keyword(s):

Wheat Straw ◽

Lentinula Edodes ◽

Medicinal Mushroom

Download Full-text

Induction of laccase activity in the white rot fungus Pleurotus ostreatus using water polluted with wheat straw extracts

Bioresource Technology ◽

10.1016/j.biortech.2013.01.072 ◽

2013 ◽

Vol 133 ◽

pp. 142-149 ◽

Cited By ~ 33

Author(s):

Alejandra Parenti ◽

Elaia Muguerza ◽

Amaia Redin Iroz ◽

Alejandra Omarini ◽

Enma Conde ◽

...

Keyword(s):

Wheat Straw ◽

Pleurotus Ostreatus ◽

Laccase Activity ◽

White Rot ◽

White Rot Fungus

Download Full-text

The reactivity of phenolic and non-phenolic residual kraft lignin model compounds with Mn(II)-peroxidase from Lentinula edodes

Bioorganic & Medicinal Chemistry ◽

10.1016/s0968-0896(99)00303-x ◽

2000 ◽

Vol 8 (2) ◽

pp. 433-438 ◽

Cited By ~ 15

Author(s):

Claudia Crestini ◽

Alessandro D'Annibale ◽

Giovanni Giovannozzi Sermanni ◽

Raffaele Saladino

Keyword(s):

Lentinula Edodes ◽

Kraft Lignin ◽

Model Compounds ◽

Lignin Model Compounds ◽

Lignin Model

Download Full-text

Ligninolytic enzyme production by Lenzites betulinus on selected plant raw materials

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1120333k ◽

2011 ◽

pp. 333-338

Author(s):

Aleksandar Knezevic ◽

Ivan Milovanovic ◽

Mirjana Stajic ◽

Jelena Vukojevic

Keyword(s):

Solid State ◽

Wheat Straw ◽

Solid State Fermentation ◽

Peroxidase Activity ◽

Submerged Fermentation ◽

Laccase Activity ◽

Raw Materials ◽

Carbon Sources ◽

Ligninolytic Enzyme ◽

Plant Residues

To get a better insight into the ligninolytic system of Lenzites betulinus, the effect of wheat straw and oak sawdust, as carbon sources, on production of Mn-oxidizing peroxidases and laccase, under solid-state and submerged fermentation, was studied. Obtained results revealed considerable differences related to the both factors affecting enzyme activities. Wheat straw was more favorable carbon source for Mn-oxidizing peroxidases and oak sawdust for laccase activity. Solid-state fermentation of wheat straw was optimal for Mn-dependent peroxidase activity (72.1 Ul-1). In contrary to this, submerged fermentation of the same residue gave the highest level of versatile peroxidase activity (25.4 Ul-1). The peak of laccase activity was noted during solid-state fermentation of oak sawdust (32.3 Ul-1), while this enzyme was not detected under submerged fermentation of any plant residues.

Download Full-text