scholarly journals Analysis of the Effect of Media Types and Chromagenic Chemicals on the Detection of Extracellular Laccase Activity among Lentinula edodes Strains

2011 ◽  
Vol 39 (1) ◽  
pp. 48-52
Author(s):  
Jun-Young Kim ◽  
Hyuk-Woo Kwon ◽  
Longqing Tang ◽  
Han-Kyu Ko ◽  
Seong-Hwan Kim
Keyword(s):  
Laccase Activity ◽  
Lentinula Edodes ◽  
Extracellular Laccase ◽  
Media Types

scholarly journals Effect of Pretreated Colza Straw on the Growth and Extracellular Ligninolytic Enzymes Production by Lentinula edodes and Ganoderma lucidum

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 157
Author(s):  
Galina Makarenkova ◽  
Valda Balode ◽  
Dzintra Zala ◽  
Elina Azena ◽  
Alexander Rapoport ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Laccase Activity ◽  
Lentinula Edodes ◽  
Culture Media ◽  
Agricultural Waste ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Solid Media ◽  
Extracellular Laccase ◽  
Surface Liquid

Lentinula edodes 3565 and Ganoderma lucidum 9621 were compared for their ability to produce lignocellulolytic enzymes in submerged (SM) and surface liquid (SL) fermentation of hydrolysed colza straw lignin waste that remained after the production of furfural and bioethanol (CS lignin). Application of cultivated mushrooms to dispose of pretreated colza straw agricultural waste is an approach to decrease the quantity of residual lignin while simultaneously obtaining active substances, e.g., the ligninolytic enzyme complex from mycelium. The effect of adding CS lignin to culture media on the yield of L. edodes and G. lucidum mycelium and extracellular laccase activity was studied. It was revealed that the mycelial growth of G. lucidum on solid media was significantly improved by adding CS lignin. Laccase activity during SL cultivation of L. edodes on medium with CS lignin gradually increased over the experiment starting on day 21 and peaked at 520 U/mL on day 28. G. lucidum expressed the maximum laccase activity, 540 U/mL, during the first 14 days of mycelium SM cultivation. Extracellular laccase activity was enhanced about 35- to 40-fold at cultivation of L. edodes and about 10- to 15-fold in the case of G. lucidum by supplementing liquid culture media with CS lignin.

scholarly journals Effect of Lignin-Containing Media on Growth of Medicinal Mushroom Lentinula Edodes

2017 ◽  
Vol 71 (1-2) ◽  
pp. 38-42
Author(s):  
Nataļja Matjuškova ◽  
Laura Okmane ◽  
Dzintra Zaļā ◽  
Linda Rozenfelde ◽  
Māris Puķe ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Nutrient Medium ◽  
Laccase Activity ◽  
Biomass Yield ◽  
Lentinula Edodes ◽  
Reference Conditions ◽  
Kraft Lignin ◽  
Mushroom Cultivation ◽  
Cultivation Medium ◽  
Solid Nutrient Medium

Abstract The effect of lignocellulose and lignin on growth of mycelium of mushroom Lentinula edodes and laccase activity in cultivation medium was studied. It was shown that cultivation of L. edodes mycelium in liquid nutrient medium with addition of 0.25-0.5% of kraft lignin increased mycelium biomass yield approximately two times compared with reference conditions without addition of lignin. Similar results were obtained in experiments in which 0.5% lignocellulose that remained after obtaining furfural, and 0.5% lignin that remained after obtaining furfural and glucose from wheat straw, were added to the nutrient medium. This effect was greater in the conditions of cultivation with good aeration, compared with static culture. Laccase activity in medium increased after addition of wheat straw lignocellulose or lignin only in the case of mycelium cultivation with aeration. In the case of mushroom cultivation on solid nutrient medium, addition of wheat straw lignocellulose and lignin promoted growth of mycelium only during the first 7 days of cultivation.

Enhanced Formation of Extracellular Laccase Activity by the White-Rot Fungus Trametes multicolor

2002 ◽  
pp. 229-241 ◽  
Author(s):  
Johann Hess ◽  
Christian Leitner ◽  
Christiane Galhaup ◽  
Klaus D. Kulbe ◽  
Barbara Hinterstoisser ◽  
...  
Keyword(s):  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Extracellular Laccase ◽  
Trametes Multicolor

scholarly journals Extra and intracellular laccase titers of xylophagic bacteria isolated from adult termite

2021 ◽  
Vol 43 ◽  
pp. e51805
Author(s):  
Luciana Furlaneto Maia ◽  
Sérgio Luis Mendes Hoyos ◽  
Alex Kiyomassa Watanabe ◽  
Alane Tatiana Pereira Moralez ◽  
Marcia Cristina Furlaneto ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Production Cost ◽  
Laccase Activity ◽  
Bacterial Isolates ◽  
Commercial Use ◽  
Extracellular Laccase ◽  
High Production ◽  
High Production Cost ◽  
Low Activity ◽  
Important Enzyme

Laccase is an important enzyme in terms of its versatile applicability, but its commercial use is limited by factors such as high production cost, low activity and/or stability under given conditions. The objective of this study was to screen xylophagic bacteria isolated from termites for the production of extracellular and intracellular laccases. Six laccase-positive strains were isolated, namely CA, A3, A5, A6, A7 and A8. They were molecularly identified by sequence analysis of 16S rRNA and classified under the genera Bacillus (A7, A8, CA) and Pseudomonas (A3, A5, A6). Laccase was produced by these bacterial isolates by submerged fermentation and was optimized at 37°C, pH 5.5, 6.2 and 7.0, with agitation and 0.5 mM guaiacol (as carbon source). Laccase activity was determined by measuring the oxidation of guaiacol and ABTS (2,21-azino bis[3-ethylbenzthiazoline-6-sulfonate]). Strain A5 produced extracellular laccase titers ranging from 123 to 168 U ml-1. Guaiacol was identified as a better substrate for the quantification of laccase. In conclusion, bacteria harboring the gut of termites can produce extracellular laccase with activity at medium to moderate acidity.

Extracellular Laccase Activity of Bacteria of the Genera Azospirillum and Niveispirillum

2019 ◽  
Vol 46 (2) ◽  
pp. 161-167
Author(s):  
E. G. Ponomareva ◽  
M. A. Kupryashina ◽  
E. P. Vetchinkina ◽  
V. E. Nikitina
Keyword(s):  
Laccase Activity ◽  
Extracellular Laccase
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