Beziehungen zwischen Vitamin B12, Purinen und Pyrimidinen bei Lactobacillus leichmannii 313

1951 ◽  
Vol 6 (1) ◽  
pp. 25-38 ◽  
Author(s):  
Friedrich Weygand ◽  
Adolf Wacker ◽  
Friedrich Wirth
Keyword(s):  
Vitamin B12 ◽  
Vitamin B ◽  
Lactobacillus Leichmannii

Abstract Es wurde das Purin-und Pyrimidinbediirfnis von Lactobacillus leichmannii 313 ermittelt. Dabei ergab sich, daß lediglich Guanin und Uracil für ein optimales Wachstum erforderlich sind. Adenin hemmt in Abwesenheit von Guanin oder Xanthin in physiologischer Konzentration das durch Vitamin B10 oder die Desoxyriboside (mit Ausnahme von Adenindesoxyribosid) hervor-gerufene Wachstum. Es wird gefolgert, daß Lb. leichmannii 313 nicht in der Lage ist, Adenin in Guanin zu verwandeln. Von einer größeren Zahl von Pyrimidinen und Benzimidazolen, die in Abwesenheit von Adenin als Hemmstoffe des Bakterienwachstums geprüft wurden, erwiesen sich 4-Amino-uracil, 4-Amino-2-thio-uracil, 5-Methyl-4-amino-2-thio-uracil, Benzimidazol, Dimethyl-, Dibrom-und Dichlorbenzimidazol als wirksam. Die durdi diese Verbindungen hervorgerufene Wachstums-hemmung von Lb. leichmannii 313 konnte kompetitiv durch Adenin oder davon sich ableitende Verbindungen, wie Adenosin und Hefeadenylsäure, nichtkompetitiv durch Vitamin B^, auf-gehoben werden. 4-Amino-uracil und 5-Methyl-4-amino-2-thio-uracil zeigten beim Mäuse-Ascitestumor eine Verzögerung der Ascitesbildung. Die Darstellung der bei den Versuchen verwendeten Benzimidazole und deren N-Glykosiden wird beschrieben. F ür gewisse Milchsäurebakterien ist Vitamin B12

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (2) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

The Vitamin B12 Content of Certain Fishery Materials

1953 ◽  
Vol 10 (2) ◽  
pp. 64-68 ◽  
Author(s):  
B. A. Southcott ◽  
H. L. A. Tarr
Keyword(s):  
Vitamin B12 ◽  
Chromatographic Separation ◽  
Growth Response ◽  
Microbiological Assay ◽  
Vitamin B ◽  
Hexane Extraction ◽  
Clupea Pallasi ◽  
Assay Procedure ◽  
Lactobacillus Leichmannii

Irregularities occurring in the microbiological assay of herring (Clupea pallasi) materials for vitamin B12 (cobalamin) are described and the probable reasons for the differences discussed. With the employment of a Lactobacillus leichmannii assay procedure and conditions under which vitamin B12 (cyano-cobalamin) and vitamin B13a (hydroxo-cobalamin) caused identical growth response, it was found that normal commercial flame-dried herring meals had about the same total cobalamin content as specially prepared meals dried at 38° to 43.5 °C. Chromatographic separation, elution and assay of the cobalamins in herring-meal samples indicated that substantially all of the activity found by direct assays of the meals was due to the vitamin itself. Hexane extraction had little effect on the cobalamin content, but heating the meals reduced it.

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (1) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

scholarly journals Molecularly imprinted membrane for transport of urea, creatinine, and vitamin B12 as a hemodialysis candidate membrane

2021 ◽  
Vol 19 (1) ◽  
pp. 806-817
Author(s):  
Muhammad Cholid Djunaidi ◽  
Nabilah Anindita Febriola ◽  
Abdul Haris
Keyword(s):  
Polyethylene Glycol ◽  
Vitamin B12 ◽  
Kidney Function ◽  
Cross Linking ◽  
Vitamin B ◽  
Molecularly Imprinted ◽  
Selective Transport ◽  
Molecularly Imprinted Membrane ◽  
Better Than ◽  
Imprinted Membrane

Abstract High levels of urea and creatinine in the blood are a sign of decreased kidney function. To remove these substances from the blood, hemodialysis which utilizes membranes could be used. In this study, a molecularly imprinted membrane (MIM) was synthesized for the selective transport of urea. The synthesis is initiated with the polymerization of eugenol into polyeugenol and then into polyeugenoxy acetate (PA). The PA is then contacted with urea and then used as the functional polymer in the synthesis of MIM with polysulfone as the membrane base, and polyethylene glycol as the cross-linking agent. The result was later analyzed with FTIR and SEM-EDX. The membrane is then used in the transport of urea, creatinine, and vitamin B12 and then compared with the non-imprinted membrane (NIM) performance. By using UV-Vis spectrophotometry, the results showed that the membrane with 10 h heating variation is able to transport more urea and is more selective than NIM; this proves that the urea template on the MIM enables it to recognize urea molecules better than creatinine and vitamin B12. The order of transport from the best results is urea > creatinine > vitamin B12.

scholarly journals Vitamin B12 and Purine Metabolism in Lactobacillus leichmannii

1963 ◽  
Vol 238 (4) ◽  
pp. 1464-1466
Author(s):  
Gary R. Craven ◽  
Mancourt Downing
Keyword(s):  
Vitamin B12 ◽  
Purine Metabolism ◽  
Lactobacillus Leichmannii

Röntgenspektroskopische Bestimmung der Wertigkeit des Kobalts im Vitamin B12

1954 ◽  
Vol 9 (8) ◽  
pp. 509-513 ◽  
Author(s):  
G. Boehm ◽  
A. Faessler ◽  
G. Rittmayer
Keyword(s):  
Vitamin B12 ◽  
Vitamin B

Mit einem fokussierenden Spektrographen großer Dispersion wird die Lage der K-Röntgen-absorptionskante des Kobalts in verschiedenen Verbindungen untersucht. Die Ergebnisse zeigen, daß es möglich ist, durch Bestimmung der Kantenlage die Wertigkeit des Kobalts in einer Verbindung zu bestimmen. Die Methode gestattet den eindeutigen Nachweis, daß das Kobalt im Vitamin B12 dreiwertig ist.

scholarly journals Effect of Vitamin-B1 and Vitamin-B12 on the Growth and Carotenoid Content of Haematococcus pluvialis CH-1

2015 ◽  
Vol 7 (9) ◽  
pp. 709-711
Author(s):  
Li-Xin Li ◽  
Zhi-Wei Song ◽  
Shun-Shan Duan ◽  
Ke-Jie Luo ◽  
Ting Li ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Haematococcus Pluvialis ◽  
Carotenoid Content ◽  
Vitamin B

Preparation of Cod Liver Residues and Vitamin B12 Concentrates

1954 ◽  
Vol 11 (4) ◽  
pp. 355-361 ◽  
Author(s):  
Beryl Truscott ◽  
D. G. Gage ◽  
P. L. Hoogland
Keyword(s):  
Vitamin B12 ◽  
Nutritive Value ◽  
Vitamin B ◽  
Sodium Bisulphite ◽  
Drum Drying ◽  
Different Types

As the first part of an investigation of the nutritive value of cod liver, two methods of preparation of cod liver residue were studied, as well as the effect of drum drying the residue, with and without the presence of sodium bisulphite, defatting the dried residue and extracting vitamin B12 from the dried residue.It was found that Vandenheuvel's method of preparation of residue produces very good results. The fresh residue could be dried without appreciable loss of vitamin B12 activity on a double drum dryer, operated with steam at 20 p.s.i.g. Sodium bisulphite afforded some protection during the drying. The most satisfactory results in defatting were obtained by extraction with dichloroethylene. Concentrates with vitamin B12 activity equivalent to 2–4 micrograms per milliliter were produced by extraction of dried, defatted residue with water and evaporation in vacuo.Five different types of residue were prepared for further studies.

scholarly journals The study of clinical profile and vitamin B 12 in dengue fever patients

2021 ◽  
Vol 12 (7) ◽  
pp. 47-51
Author(s):  
Mamatha Tittamegalapalya Ramalingaiah ◽  
Jeetendra Kumar Jogihalli Mood ◽  
Satyanarayana Narayanashetty ◽  
Rashmi Madappa Bhuvaneshappa
Keyword(s):  
Vitamin B12 ◽  
Hospital Stay ◽  
Dengue Fever ◽  
Platelet Transfusion ◽  
Clinical Profile ◽  
Study Patient ◽  
Vitamin B ◽  
Severe Thrombocytopenia ◽  
Duration Of Hospital Stay ◽  
Vitamin B 12

Background: Dengue is an acute infectious febrile illness characterised by thrombocytopenia and platelet dysfunction leading to bleeding manifestations. Vitamin B12 is required for platelet production in the bone marrow. So, deficiency of vitamin B12 in dengue patients can have severe thrombocytopenia. Aims and Objective: Study was aimed to know the clinical profile of dengue fever patients and to correlate serum vitamin B12 level with severity of thrombocytopenia, platelet transfusion and duration of hospital stay. Materials and Methods: This observational study was done on dengue patients for period of 3 months January 2020 to March 2020. Confirmed cases of dengue fever with NS 1 Ag positive & Ig M antibody positive were included in the study. Patient with sepsis, underlying malignancy, autoimmune disorder, hematological disorder, drugs causing thrombocytopenia were excluded from the study. Appropriate statistical methods were applied. Results: Total 50 subjects were included. Majority of subjects were in the age group 31 to 40 years. 50% were males and females respectively. Most common clinical feature was fever and bleeding manifestations. Mean Vitamin B12 was significantly lower among those with severe thrombocytopenia and highest among those with no thrombocytopenia., there was significant difference in mean Platelet transfusion and duration of hospital stay with respect to severity of Platelet count. Conclusion: Dengue fever patients with vitamin B 12 deficiency had moderate to severe thrombocytopenia and more bleeding manifestations. Those patients required more platelet transfusion and increased duration of hospital stay.

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