The Nature of Monkey Cell Haemagglutinins of Rosen’s Group II Adenoviruses
Some of the adenovirus type of Rosen’s group II exhibit weak agglutinating properties for monkey blood cells. The relation of these haemagglutinins to haemagglutinins for rat blood cells and to the infectious virus was studied by cross-absorption with blood cells and by density gradient centrifugation with caesium chloride. In the virus types studied (type 9, 13, and a wild strain of type 15) one part of the monkey cell haemagglutinin was associated with the virion and, for type 9 and 15, was inseparable from the virus-associated haemagglutinin for rat blood cells. In density gradient centrifugation a second haemagglutinin for monkey blood cells was found, separable from the virus particles and associated with the portion of higher buoyant density of the soluble haemagglutinin for rat blood cells. In type 13 and 23, haemagglutinins for monkey and rat blood cells can be separated by absorption with the heterologous blood cell species.Haemagglutinins for monkey cells are resistant to trypsin or trypsin plus subsequent heating to 55°C, in contrast to haemagglutinins for rat blood cells. Monkey cell haemagglutination is reversibly inhibited by the presence of CsCl.In haemagglutination-inhibition tests it was found that haemagglutination with monkey blood cells is more difficult to be inhibited by immune serum that haemagglutination with rat blood cells.