Absence of Plastocyanin in the Alga Bumilleriopsis and its Replacement by Cytochrome 553

1975 ◽  
Vol 30 (3-4) ◽  
pp. 190-200 ◽  
Author(s):  
Karl-Josef Kunert ◽  
Peter Böger
Keyword(s):  
Chlorella Vulgaris ◽  
Spinacia Oleracea ◽  
Cytochrome F ◽  
Spinach Chloroplast ◽  
Isolation And Purification ◽  
Copper Protein ◽  
Pure Protein ◽  
Photosynthetic Reactions ◽  
Induced Changes ◽  
Spectral Assessment

Abstract Different preparative procedures for isolation and purification of plastocyanin were successful to obtain the pure protein from Chlorella vulgaris, Scenedesm us acutus and Spinacia oleracea but not from B um illeriopsis filiform is. Lade of plastocyanin in this alga was further confirmed by the following in comparison with spinach chloroplast material: a. Use of antibodies to block photosynthetic reactions generally dependent on plastocyanin; b. epr spectral assessment; c. treatment of chloroplasts with KCN and amphotericin B ; d. light-induced changes at 553 nm ( = cytochrome f photooxidation). The latter assays indicated that, in contrast to spinach, neither plastocyanin nor cytochrome f ( = Cyt 553) is present in sonified or pyridine treated Bumilleriopsis chloroplasts. Photooxidation of (added) cytochrome 553 takes place not only without plastocyanin but is counteracted by the copper protein

scholarly journals Isolation and purification of chloroplastic spinach (Spinacia oleracea) sedoheptulose-1,7-bisphosphatase

1987 ◽  
Vol 241 (1) ◽  
pp. 71-74 ◽  
Author(s):  
F Cadet ◽  
J C Meunier ◽  
N Ferté
Keyword(s):  
Affinity Chromatography ◽  
Spinacia Oleracea ◽  
Higher Plant ◽  
Isolation And Purification ◽  
Fructose 1,6 Bisphosphate ◽  
Molecular Sieving

Higher-plant sedoheptulose-1,7-bisphosphatase was isolated and purified over 200-fold from spinach (Spinacia oleracea) chloroplast stromal extracts to apparent electrophoretic homogeneity by DEAE-Fractogel, molecular sieving on Sephadex G-200 and Blue B dye-matrix affinity chromatography. It is a protein of Mr 66,000, made up of two apparently identical subunits (Mr 35,000). The enzyme is activated by reduced thioredoxin fb in the presence of dithiothreitol. Its specificity towards sedoheptulose 1,7-bisphosphate versus fructose 1,6-bisphosphate is high, but not absolute.

Effect of induced changes in membrane permeability on the defence response of Chlorella vulgaris to infection by Acholeplasma laidlawii

2004 ◽  
Vol 422 (1-2) ◽  
pp. 95-100 ◽  
Author(s):  
N. Loseva ◽  
L. Gordon ◽  
A. Alyabyev ◽  
I. Andreyeva ◽  
O. Kolesnikov ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Chlorella Vulgaris ◽  
Defence Response ◽  
Acholeplasma Laidlawii ◽  
Induced Changes

scholarly journals Fluoranthene induced changes in photosynthetic pigments, biochemical compounds and enzymatic activities in two microalgal species: Chlorella vulgaris Beijerinck and Desmodesmus subspicatus Chodat

2014 ◽  
Vol 3 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Miral Patel ◽  
JI Nirmal Kumar ◽  
KK Tiwari
Keyword(s):  
Chlorella Vulgaris ◽  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Enzymatic Activities ◽  
Variable Amount ◽  
Biochemical Compounds ◽  
Desmodesmus Subspicatus ◽  
Metabolic Activities ◽  
Induced Changes ◽  
Microalgal Species

The photosynthetic pigments, biochemical and enzymatic activities in two freshwater microalgal species, Chlorella vulgaris and Desmodesmus subspicatus at different fluoranthene concentrations were compared with the control conditions. During 16-days of incubation period when treated with fluoranthene, both microalgal species exhibited variable amount of photosynthetic pigment, biochemical compounds and enzymatic activities. The addition of fluoranthene at concentrations ranged from 1.5 mg l-1; to 10 mg l-1; to microalgal cultures led to changes in all different metabolites but the patterns varied from species to species. Among the two species tested, pigment, biochemical and enzymatic contents were remarkably declined from 7 % to 95% in C. vulgaris. Moreover, all metabolites in D. subspicatus also diminishing significantly by 3% to 88% of fluoranthene doses (10ppm). These results suggest that fluoranthene-induced changes of pigments, biochemical and enzymatic variations in test microalgae, D. subspicatus and C. vulgaris, might reveal its resistance and ability to metabolize PAHs. At the same time, the PAH impact changes on different metabolic activities were higher at 12 and 16 days than at 4 and 8 days in treated microalgae. DOI: http://dx.doi.org/10.3126/ije.v3i1.9941 International Journal of Environment Vol.3(1) 2014: 41-55

Interpretation of irradiation-induced changes in electron diffractograms and images of extinction contours at low temperatures

1984 ◽  
Vol 42 ◽  
pp. 268-269
Author(s):  
E. Knapek ◽  
H. Formanek ◽  
G. Lefranc ◽  
I. Dietrich
Keyword(s):  
Low Temperatures ◽  
Carbon Films ◽  
Organic Crystals ◽  
Wide Spread ◽  
Lens System ◽  
Preparation Conditions ◽  
Thin Carbon ◽  
Electron Diffractograms ◽  
Diffraction Patterns ◽  
Induced Changes

A few years ago results on cryoprotection of L-valine were reported, where the values of the critical fluence De i.e, the electron exposure which decreases the intensity of the diffraction reflections by a factor e, amounted to the order of 2000 + 1000 e/nm2. In the meantime a discrepancy arose, since several groups published De values between 100 e/nm2 and 1200 e/nm2 /1 - 4/. This disagreement and particularly the wide spread of the results induced us to investigate more thoroughly the behaviour of organic crystals at very low temperatures during electron irradiation.For this purpose large L-valine crystals with homogenuous thickness were deposited on holey carbon films, thin carbon films or Au-coated holey carbon films. These specimens were cooled down to nearly liquid helium temperature in an electron microscope with a superconducting lens system and irradiated with 200 keU-electrons. The progress of radiation damage under different preparation conditions has been observed with series of electron diffraction patterns and direct images of extinction contours.

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