Light induced changes in the lipids of Chlorella vulgaris

The photosynthetic pigments, biochemical and enzymatic activities in two freshwater microalgal species, Chlorella vulgaris and Desmodesmus subspicatus at different fluoranthene concentrations were compared with the control conditions. During 16-days of incubation period when treated with fluoranthene, both microalgal species exhibited variable amount of photosynthetic pigment, biochemical compounds and enzymatic activities. The addition of fluoranthene at concentrations ranged from 1.5 mg l-1; to 10 mg l-1; to microalgal cultures led to changes in all different metabolites but the patterns varied from species to species. Among the two species tested, pigment, biochemical and enzymatic contents were remarkably declined from 7 % to 95% in C. vulgaris. Moreover, all metabolites in D. subspicatus also diminishing significantly by 3% to 88% of fluoranthene doses (10ppm). These results suggest that fluoranthene-induced changes of pigments, biochemical and enzymatic variations in test microalgae, D. subspicatus and C. vulgaris, might reveal its resistance and ability to metabolize PAHs. At the same time, the PAH impact changes on different metabolic activities were higher at 12 and 16 days than at 4 and 8 days in treated microalgae. DOI: http://dx.doi.org/10.3126/ije.v3i1.9941 International Journal of Environment Vol.3(1) 2014: 41-55

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Absence of Plastocyanin in the Alga Bumilleriopsis and its Replacement by Cytochrome 553

Zeitschrift für Naturforschung C ◽

10.1515/znc-1975-3-409 ◽

1975 ◽

Vol 30 (3-4) ◽

pp. 190-200 ◽

Cited By ~ 29

Author(s):

Karl-Josef Kunert ◽

Peter Böger

Keyword(s):

Chlorella Vulgaris ◽

Spinacia Oleracea ◽

Cytochrome F ◽

Spinach Chloroplast ◽

Isolation And Purification ◽

Copper Protein ◽

Pure Protein ◽

Photosynthetic Reactions ◽

Induced Changes ◽

Spectral Assessment

Abstract Different preparative procedures for isolation and purification of plastocyanin were successful to obtain the pure protein from Chlorella vulgaris, Scenedesm us acutus and Spinacia oleracea but not from B um illeriopsis filiform is. Lade of plastocyanin in this alga was further confirmed by the following in comparison with spinach chloroplast material: a. Use of antibodies to block photosynthetic reactions generally dependent on plastocyanin; b. epr spectral assessment; c. treatment of chloroplasts with KCN and amphotericin B ; d. light-induced changes at 553 nm ( = cytochrome f photooxidation). The latter assays indicated that, in contrast to spinach, neither plastocyanin nor cytochrome f ( = Cyt 553) is present in sonified or pyridine treated Bumilleriopsis chloroplasts. Photooxidation of (added) cytochrome 553 takes place not only without plastocyanin but is counteracted by the copper protein

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Interpretation of irradiation-induced changes in electron diffractograms and images of extinction contours at low temperatures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111458 ◽

1984 ◽

Vol 42 ◽

pp. 268-269

Author(s):

E. Knapek ◽

H. Formanek ◽

G. Lefranc ◽

I. Dietrich

Keyword(s):

Low Temperatures ◽

Carbon Films ◽

Organic Crystals ◽

Wide Spread ◽

Lens System ◽

Preparation Conditions ◽

Thin Carbon ◽

Electron Diffractograms ◽

Diffraction Patterns ◽

Induced Changes

A few years ago results on cryoprotection of L-valine were reported, where the values of the critical fluence De i.e, the electron exposure which decreases the intensity of the diffraction reflections by a factor e, amounted to the order of 2000 + 1000 e/nm2. In the meantime a discrepancy arose, since several groups published De values between 100 e/nm2 and 1200 e/nm2 /1 - 4/. This disagreement and particularly the wide spread of the results induced us to investigate more thoroughly the behaviour of organic crystals at very low temperatures during electron irradiation.For this purpose large L-valine crystals with homogenuous thickness were deposited on holey carbon films, thin carbon films or Au-coated holey carbon films. These specimens were cooled down to nearly liquid helium temperature in an electron microscope with a superconducting lens system and irradiated with 200 keU-electrons. The progress of radiation damage under different preparation conditions has been observed with series of electron diffraction patterns and direct images of extinction contours.

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Drug Induced Changes in Cell Organelles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100299 ◽

1968 ◽

Vol 26 ◽

pp. 110-111

Author(s):

Sarah A. Luse

Keyword(s):

Clinical Medicine ◽

Cell Organelles ◽

Riboflavin Deficiency ◽

Drug Induced ◽

New Era ◽

Health And Disease ◽

In The Beginning ◽

Cellular Pathology ◽

Induced Changes ◽

The Impact

In the mid-nineteenth century Virchow revolutionized pathology by introduction of the concept of “cellular pathology”. Today, a century later, this term has increasing significance in health and disease. We now are in the beginning of a new era in pathology, one which might well be termed “organelle pathology” or “subcellular pathology”. The impact of lysosomal diseases on clinical medicine exemplifies this role of pathology of organelles in elucidation of disease today.Another aspect of cell organelles of prime importance is their pathologic alteration by drugs, toxins, hormones and malnutrition. The sensitivity of cell organelles to minute alterations in their environment offers an accurate evaluation of the site of action of drugs in the study of both function and toxicity. Examples of mitochondrial lesions include the effect of DDD on the adrenal cortex, riboflavin deficiency on liver cells, elevated blood ammonia on the neuron and some 8-aminoquinolines on myocardium.

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Digital x-ray mapping of nanometer-size supported bimetallic catalysts

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100104716 ◽

1988 ◽

Vol 46 ◽

pp. 530-531

Author(s):

L. T. Germinario

Keyword(s):

Background Radiation ◽

Metal Cluster ◽

Single Element ◽

Beam Diameter ◽

X Rays ◽

X Ray ◽

Major Interest ◽

Induced Changes

Understanding the role of metal cluster composition in determining catalytic selectivity and activity is of major interest in heterogeneous catalysis. The electron microscope is well established as a powerful tool for ultrastructural and compositional characterization of support and catalyst. Because the spatial resolution of x-ray microanalysis is defined by the smallest beam diameter into which the required number of electrons can be focused, the dedicated STEM with FEG is the instrument of choice. The main sources of errors in energy dispersive x-ray analysis (EDS) are: (1) beam-induced changes in specimen composition, (2) specimen drift, (3) instrumental factors which produce background radiation, and (4) basic statistical limitations which result in the detection of a finite number of x-ray photons. Digital beam techniques have been described for supported single-element metal clusters with spatial resolutions of about 10 nm. However, the detection of spurious characteristic x-rays away from catalyst particles produced images requiring several image processing steps.

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Immunogold localization of calmodulin in root caps of the maize Cultivar Merit

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160923 ◽

1990 ◽

Vol 48 (3) ◽

pp. 674-675

Author(s):

P.T. Nguyen ◽

C. Uphoff ◽

C.L. Stinemetz

Keyword(s):

Calcium Binding ◽

Calcium Transport ◽

Primary Role ◽

Immunogold Localization ◽

Root Tips ◽

Electrical Currents ◽

Considerable Evidence ◽

Maize Cultivar ◽

Gravity Response ◽

Induced Changes

Considerable evidence suggest that the calcium-binding protein calmodulin (CaM) may mediate calcium action and/or transport important in the gravity response of plants. Calmodulin is present in both shoots and roots and is capable of regulating calcium transport in plant vesicles. In roots calmodulin is concentrated in the tip, the gravisensing region of the root; and is reported to be closely associated with amyloplasts, organelles suggested to play a primary role in gravi-perception. Inhibitors of CaM such as chlorpromazine, calmidazolium, and compound 48/80 interfere with the gravitropic response of both snoots and roots. The magnitude of the inhibition corresponded well with the extent to which the drug binds to endogenous CaM. Compound 48/80 and calmidazolium block gravi-induced changes in electrical currents across root tips, a phenomenon thought to be associated with the sensing of the gravity stimulus.In this study, we have investigated the subcellular distribution of CaM in graviresponsive and non-graviresponsive root caps of the maize cultivar Merit.

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Identifying groups of airborne particles by ordination

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100172012 ◽

1994 ◽

Vol 52 ◽

pp. 856-857

Author(s):

M. Shlepr ◽

C. M. Vicroy

Keyword(s):

Elemental Analysis ◽

Energy Dispersive Spectroscopy ◽

Manufacturing Process ◽

Spatial Representation ◽

Airborne Particles ◽

Common Source ◽

Data Set ◽

Microelectronics Industry ◽

Induced Changes ◽

Source Materials

The microelectronics industry is heavily tasked with minimizing contaminates at all steps of the manufacturing process. Particles are generated by physical and/or chemical fragmentation from a mothersource. The tools and macrovolumes of chemicals used for processing, the environment surrounding the process, and the circuits themselves are all potential particle sources. A first step in eliminating these contaminants is to identify their source. Elemental analysis of the particles often proves useful toward this goal, and energy dispersive spectroscopy (EDS) is a commonly used technique. However, the large variety of source materials and process induced changes in the particles often make it difficult to discern if the particles are from a common source.Ordination is commonly used in ecology to understand community relationships. This technique usespair-wise measures of similarity. Separation of the data set is based on discrimination functions. Theend product is a spatial representation of the data with the distance between points equaling the degree of dissimilarity.

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