Two Phases Of Ferricyanide Reductase Activity In Ehrlich Cell Plasma Membranes

1992 ◽  
Vol 47 (11-12) ◽  
pp. 929-931 ◽  
Author(s):  
Antonio del Castillo-Olivares ◽  
Javier Márquez ◽  
Ignacio Núñez de Castro ◽  
Miguel Angel Medina
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Reductase Activity ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Cell Plasma Membrane ◽  
Ferricyanide Reductase ◽  
Ehrlich Cell ◽  
Cell Plasma ◽  
Two Phases

Ehrlich cell plasma membrane vesicles have a ferricyanide reductase activity that shows two phases. These two phases were kinetically characterized. Evidence is presented for a differential effect of trypsin on both phases

scholarly journals Diamines interfere with the transport of l-ornithine in Ehrlich-cell plasma-membrane vesicles

1991 ◽  
Vol 280 (3) ◽  
pp. 825-827 ◽  
Author(s):  
M A Medina ◽  
J L Urdiales ◽  
J M Mates ◽  
I Núñez de Castro ◽  
F Sánchez-Jiménez
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Cell Plasma Membrane ◽  
Ehrlich Cells ◽  
Ehrlich Cell ◽  
Cell Plasma ◽  
Diffusional Component

1. L-Ornithine transport by plasma-membrane vesicles isolated from Ehrlich cells is Na(+)-independent and shows a saturable and a diffusional component. 2. Putrescine, histamine, 5-hydroxytryptamine and 2,3-diaminopropane at 55 microM concentration significantly inhibit 0.5 mM-L-ornithine transport at least for the first 10 min of incubation. 3. There is a trans-stimulatory effect of putrescine on L-ornithine transport.

p-Chloromercurobenzene sulfonate inhibition of active Cl- transport in plasma membrane vesicles from Aplysia gut

1990 ◽  
Vol 259 (6) ◽  
pp. R1111-R1116
Author(s):  
G. A. Gerencser
Keyword(s):  
Plasma Membrane ◽  
Transport Process ◽  
Transport Mechanism ◽  
Plasma Membranes ◽  
Membrane Vesicles ◽  
Sucrose Density Gradient ◽  
Absorptive Cell ◽  
Plasma Membrane Vesicles ◽  
Cl Transport ◽  
Cell Plasma

Both a Cl(+)-stimulated adenosinetriphosphatase (ATPase) activity and an ATP-dependent Cl- transport process were found in Aplysia foregut absorptive cell plasma membranes. In an attempt to further characterize this transport process, plasma membrane vesicles from Aplysia foregut absorptive cells were prepared utilizing differential centrifugation and sucrose density-gradient techniques. Sulfhydryl ligand participation in ATP-dependent Cl- transport was confirmed in three ways. First, 1,4-dithiothreitol partially restored a p-chloromercurobenzene sulfonate (PCMBS)-inhibited ATP-dependent Cl- transport. Second, 1,4-dithiothreitol restored intravesicular negativity inhibited by PCMBS. Third, 1,4-dithiothreitol had no effect on either ATP-dependent Cl- transport or ATP-dependent intravesicular negativity inhibited by N-ethylmaleimide. These results are consistent with the hypothesis that surface sulfhydryl groups participate in the functioning of the active electrogenic Cl- transport mechanism in Aplysia gut.

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