Artificial Alterations of Fluidity of Pea Thylakoid Membranes and Its Effect on Energy Distribution between Both Photosystems

1997 ◽  
Vol 52 (7-8) ◽  
pp. 475-480 ◽  
Keyword(s):  
Light Scattering ◽  
Energy Distribution ◽  
Benzyl Alcohol ◽  
Membrane Fluidity ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Scattering Intensity ◽  
Lower Extent ◽  
Light Scattering Intensity ◽  
Pigment Protein Complexes

Abstract Two different membrane perturbing agents -cholesterol and benzyl alcohol were applied to modify the fluidity of pea thylakoid membranes. Well pronounced decrease of membrane fluidity was observed upon increasing of cholesterol added. Rigification of thylakoid membranes was accompanied by reduction of cation-induced increase of light-scattering intensity and results in lower extent of increase of spillover upon cation induced stack-unstack transi­tion. Thylakoid membranes, treated with local anesthetic benzyl alcohol, showed an increase of membrane fluidity, but approximately the same behavior as untreated thylakoids in respect to the light scattering intensity dependence on cation concentration. Upon stack-unstack transition the observed changes of energy distribution for benzyl alcohol treated thylakoids are similar to those for controls. The data are discussed in terms of influence of membrane fluidity on lateral reorganization of pigment-protein complexes.

Light Scattering Intensity Fluctuations in Microdroplets Containing Inclusions.

1997 ◽  
Author(s):  
Gorden Videen ◽  
Paul Pellegrino ◽  
Dat Ngo ◽  
John S. Videen ◽  
Ronald G. Pinnick
Keyword(s):  
Light Scattering ◽  
Scattering Intensity ◽  
Light Scattering Intensity ◽  
Intensity Fluctuations

Some Effects on Light Scattering Intensity and Red Blood Cell Size Distribution Histograms Due to Sphering

1988 ◽  
Vol 25 (6) ◽  
pp. 673-679 ◽  
Author(s):  
P C Bartels ◽  
P W Helleman ◽  
A F M Roijers ◽  
J B J Soons
Keyword(s):  
Light Scattering ◽  
Blood Cell ◽  
Size Distribution ◽  
Red Blood Cell ◽  
Signal To Noise Ratio ◽  
Quantitative Information ◽  
Cell Size Distribution ◽  
Scattering Intensity ◽  
Distribution Ranges ◽  
Light Scattering Intensity

At present most haematology blood cell analysers routinely provide red blood cell (RBC) size distribution histograms. Sophisticated improvements of the instruments have re-awakened interest in the study of size histograms. The quantitative information derived from the histograms may be applied more fruitfully if insight is available, with respect to some essential principles of sizing technology and methods for treatment of RBCs before measurement. In this study the consequences of sphering RBCs are investigated in relation to the generation of size distribution histograms by means of methods based on light scattering intensity (LSI). Sphering of RBCs results in considerably narrower histograms than unsphered RBCs. The overall signal to noise ratio increases and there is a broader gap between large platelets and microcytic RBCs. Narrower size distribution ranges will enable closer modes to be separated. Compared to unsphered RBCs, microcytic sphered RBCs yield increased LSI whereas macrocytic sphered RBCs yield decreased LSI.

A Fiber Optic Particle Plasmon Resonance Biosensing Platform Based on Detection of Light Scattering Intensity from the Proximal End

2011 ◽  
Vol 58 (6) ◽  
pp. 786-792 ◽  
Author(s):  
Lai-Kwan Chau ◽  
Chia-Wen Kuo ◽  
Ya-Wei Chu ◽  
Shin-Hsien Liao ◽  
Yung-Tai Lin ◽  
...  
Keyword(s):  
Light Scattering ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Scattering Intensity ◽  
Light Scattering Intensity ◽  
Particle Plasmon
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