Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis

2010 ◽  
Vol 48 (3) ◽  
pp. 475-480
Author(s):  
V. Karlicky ◽  
J. Podolinska ◽  
L. Nadkanska ◽  
M. Stroch ◽  
M. Cajanek ◽  
...  
Keyword(s):  
Functional State ◽  
Gel Electrophoresis ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Pigment Composition ◽  
Pigment Protein Complexes

Strong light-induced reorganization of pigment–protein complexes of thylakoid membranes in rye (spectroscopic study)

2012 ◽  
Vol 169 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Ewa Janik ◽  
Waldemar Maksymiec ◽  
Wojciech Grudziński ◽  
Wiesław I. Gruszecki
Keyword(s):  
Spectroscopic Study ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Strong Light ◽  
Pigment Protein Complexes

scholarly journals Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

2012 ◽  
Vol 161 (1) ◽  
pp. 497-507 ◽  
Author(s):  
Helmut Kirchhoff ◽  
Richard M. Sharpe ◽  
Miroslava Herbstova ◽  
Robert Yarbrough ◽  
Gerald E. Edwards
Keyword(s):  
Protein Complexes ◽  
Thylakoid Membranes ◽  
C4 Plants ◽  
C3 And C4 Plants ◽  
Differential Mobility ◽  
Pigment Protein Complexes

Occurrence of Secondary Carotenoids in PS I Complexes Isolated from Eremosphaera viridis De Bary (Chlorophyceae)

1992 ◽  
Vol 47 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
Burkhard Vechtel ◽  
Elfriede K. Pistorius ◽  
Hans Georg Ruppel
Keyword(s):  
Green Algae ◽  
Photosystem I ◽  
Protein Complexes ◽  
Pigment Composition ◽  
Eremosphaera Viridis ◽  
Ps I ◽  
Pigment Protein Complexes ◽  
Secondary Carotenoids ◽  
Lhc I ◽  
Β Carotene

Abstract Photosystem I complexes of Eremosphaera viridis De Bary (Chlorophyceae, Chlorococcales) were isolated and partially characterized. In the isolated PS I complexes, peptides of 64-60, 26, 23, 20, 15, 11 and 8.5 kDa could be detected. When Eremosphaera was grown under regular conditions the pigment composition of the isolated PS I complexes was similar to that found in PS I complexes from other green algae. However, when Eremosphaera was grown under nitrogen deficient conditions, PS I complexes contained the secondary carotenoids canthaxanthin and traces of astaxanthin and echinenone in addition to β-carotene, violaxanthin and lutein. The results presented indicate that the secondary carotenoids are associated with the LHC I of PS I. To our knowledge this represents the first report about the association of secondary carotenoids with light harvesting pigment protein complexes of green algae.

Chlorophyll Photobleaching in Pigment-Protein Complexes

1986 ◽  
Vol 41 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Robert Carpentier ◽  
Roger M. Leblanc ◽  
Guy Bellemare
Keyword(s):  
Energy Transfer ◽  
Hordeum Vulgare ◽  
Chlamydomonas Reinhardtii ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Wild Type ◽  
Protection Mechanism ◽  
Long Wavelength ◽  
Pigment Protein Complexes ◽  
Type Mutant

Pigment photobleaching was performed in thylakoid membranes of Hordeum vulgare (wild type, mutant Chlorina f2, Norfluranzon treated seedlings) and in pigment-protein complexes (CP-I and LHCP) isolated from H. vulgare and Chlamydomonas reinhardtii. Multiphasic kinetics were obtained in all of the above cases. Energy transfer towards pigments absorbing at longer wavelength is postulated as a general protection mechanism against photobleaching. This mechanism explains a substantial bleaching of carotenoids and a faster bleaching of chlorophyll aggregates, absorbing at long wavelength. These conclusions were valid for isolated complexes as well as for thylakoid membranes, although membranes were less sensitive to light.

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