Enzymatic Hydrolysis of Carotenoid Fatty Acid Esters of Red Pepper (Capsicum annuum L.) by a Lipase from Candida rugosa

2000 ◽  
Vol 55 (11-12) ◽  
pp. 971-975 ◽  
Author(s):  
Dietmar Ernst Breithaupt
Keyword(s):  
Enzymatic Hydrolysis ◽  
Candida Rugosa ◽  
Fatty Acid Esters ◽  
Red Pepper ◽  
Free Form ◽  
Enzymatic Cleavage ◽  
Reaction Conditions ◽  
First Time ◽  
Hydrolysis Of ◽  
Acid Esters

Analyses of red pepper extracts which had been pretreated with lipase type VII (EC 3.1.1.3.) from Candida rugosa showed for the first time pepper carotenoid esters to be substrates of this enzyme. However, the extent of enzymatic hydrolysis depends on the respective carotenoid and was not quantitative compared to chemical saponification. After enzymatic cleavage, 67-89% of total capsanthin, 61-65% of total zeaxanthin, 70-81% of total β-cryptoxanthin and 70-86% of total violaxanthin were detected in free form. Nevertheless, the method described here offers the possibility to cleave in part several carotenoid esters originating from red pepper quickly and under comparatively mild reaction conditions. Replacement of the generally performed alkaline hydrolysis by enzymatic cleavage allows the resulting product to be used in food industry as “natural” coloring agent e.g. to colour cheese and jellies.

Hydrolysis of Xenobiotic Fatty Acid Esters by Carboxylesterases of Human Skin

1994 ◽  
pp. 75-83
Author(s):  
Eberhard Heymann ◽  
Felicitas Noetzel ◽  
Rita Retzlaff ◽  
Gabriele Schnetgöke ◽  
Sonja Westie
Keyword(s):  
Fatty Acid ◽  
Human Skin ◽  
Fatty Acid Esters ◽  
Hydrolysis Of ◽  
Acid Esters

scholarly journals Isolation of Free Amino Acids via Enzymatic Hydrolysis of Tobacco-Derived F1 Protein

2018 ◽  
Vol 28 (4) ◽  
pp. 179-190
Author(s):  
Mehdi Ashraf-Khorassani ◽  
William M. Coleman ◽  
Michael F. Dube ◽  
Larry T. Taylor
Keyword(s):  
Amino Acids ◽  
Enzymatic Hydrolysis ◽  
Free Amino Acids ◽  
Free Amino ◽  
Protein Concentration ◽  
Enzyme Concentration ◽  
Enzymatic Conversion ◽  
Reaction Conditions ◽  
Analytical Methodology ◽  
Hydrolysis Of

SummaryFree amino acids have been isolated via optimized enzymatic hydrolysis of F1 tobacco protein using two cationic resins (Amberlite IR120 and Dowex MAC-2). Optimized enzymatic conversions of the protein as a result of systematic variations in conditions (e.g., time, temperature, pH, enzyme type, enzyme concentration, anaerobic/aerobic environments, and protein concentration) employing commercially available enzymes, were consistently higher than 50% with qualitative amino acid arrays that were consistent with the known composition of tobacco F1 protein. Amberlite IR120 was shown to have a much higher efficiency and capacity for isolation of amino acids from standard solutions and from hydrolysate when compared with the results using Dowex MAC-2. Two columns packed with conditioned Amberlite IR120 (120 × 10 mm,12–15 g resin) and (200 × 25.4 mm, 60–65 g resin) were used to isolate two batches (2.5–3.0 mg and 13–15 mg) of free amino acids, respectively. A relatively inexpensive analytical methodology was developed for rapid analysis of the free amino acids contained within the enzyme hydrolysate. Commercially available enzymes, when employed in optimized reaction conditions, are very effective for enzymatic conversion of tobacco F1 protein to free amino acids.

scholarly journals Microwave-Assisted Catalytic Synthesis of Bio-Based Copolymers from Waste Cooking Oil

2017 ◽  
Author(s):  
Mahrzadi Noureen Shahi ◽  
Muhammad Arshad ◽  
Aman Ullah
Keyword(s):  
Molecular Weight ◽  
Gel Permeation Chromatography ◽  
Waste Cooking Oil ◽  
Attenuated Total Reflection ◽  
Fatty Acid Esters ◽  
Proton Nuclear Magnetic Resonance ◽  
Co Catalyst ◽  
Cooking Oil ◽  
First Time ◽  
Acid Esters

Solvent free copolymerization of epoxides derived from fatty acid esters of waste cooking oil with phthalic anhydride using (salen)CrIII Cl as catalyst and n-Bu4NCl/DMAP as co-catalyst was carried out for the first time under microwave irradiation, where reaction time was reduced from number of hours to minutes. The polyesters were obtained with molecular weight (Mw = 3084-6740 g/mol) and dispersity values (D = 1.18-1.92), when (salen)CrIII Cl/n-Bu4NCl was used as catalysts. While in case of DMAP as a co-catalyst, polyesters with improved molecular weight (Mw = 5537-6925 g/mol) and narrow dispersity values (D = 1.07-1.28) were obtained even at reduced concentrations of (salen)CrIII Cl and DMAP. The obtained products were characterized and evaluated by attenuated total reflection-fourier transform infrared spectroscopy (ATR-FTIR), proton nuclear magnetic resonance (1H-NMR) spectroscopy, gel permeation chromatography (GPC) and thermogravimetric analysis (TGA) Techniques.

scholarly journals SELECTIVITY OF CANDIDA RUGOSA LIPASE IMMOBILIZED ONTO LAYERED DOUBLE HYDROXIDES AS CATALYST IN SYNTHESIS OF FATTY ACID ESTERS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Mohd Basyaruddin Abdul Rahman ◽  
Siti Salhah Othman ◽  
Noor Mona Md Yunus
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chain Length ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Carbon Chain ◽  
Fatty Acid Esters ◽  
Carbon Chain Length ◽  
Chain Lengths ◽  
Acid Esters

The enzymatic selectivity of Lipase from Candida rugosa immobilized onto a calcined layered double hydroxide (CLDHs-CRL) towards the chain-length of fatty acids and alcohols in the synthesis of fatty acid esters was investigated.  The results showed that CMAN-CRL catalyzed the esterification process with fatty acids of medium chain lengths (C10-C14) effectively while, CNAN-CRL and CZAN-CRL exhibited high percentage conversion in fatty acids with carbon chain lengths of C8-C12 and C10-C18, respectively. In the alcohol selectivity study, CMAN-CRL showed high selectivity toward alcohols with carbon chain lengths of C4, C6 and C10.  On the other hand, both CNAN-CRL and CZAN-CRL exhibited rather low selectivity towards longer carbon chain length of alcohols. 

Inhibition of proximal tubular hydrolysis and reabsorption of bradykinin by peptides

1976 ◽  
Vol 231 (3) ◽  
pp. 743-748 ◽  
Author(s):  
S Oparil ◽  
FA Carone ◽  
TN Pullman ◽  
S Nakamura
Keyword(s):  
Enzymatic Hydrolysis ◽  
High Capacity ◽  
High Specificity ◽  
Proximal Tubules ◽  
Enzymatic Cleavage ◽  
Angiotensin I ◽  
Urinary Recovery ◽  
Proximal Tubular ◽  
Hydrolysis Of ◽  
Fold Excess

[3H] bradykinin ([3H] BKN) was microinfused alone or in the presence of a 390- or 780-fold excess of BKN or angiotensin I (AI) into proximal tubules in Inactin-anesthetized rats. Urinary excretion of 3H-labeled material was measured, and intact peptide and its metabolites were identified and quantified. When [3H] BKN was administered with BKN or AI, urinary recovery of 3H-labeled material was increased in a manner directly proportional to tubular length, suggesting that reabsorption of [3H] BKN is related to extent of tubular contact. BKN and AI were equally effective in inhibiting the reabosroption of [3H] BKN and its metabolites from proximal tubular fluid. In contrast, BKN but not AI effectively inhibited the enzymatic hydrolysis of [3H] BKN in the proximal tubule, The data suggest that the proximal tubular mechanism for reabsorbing BKN and its metabolites is of high capacity but not high specificity and that the mechanisms for enzymatic cleavage and reabsorption of BKN and its metabolites may had different specificites and capacities.

Hydrolysis of Fatty Acid Esters byCandida AntarcticaLipase B (Novozym 435) Dissolved in Anhydrous Triethylamine

ChemCatChem ◽  
2012 ◽  
Vol 4 (12) ◽  
pp. 2050-2054 ◽  
Author(s):  
Markus Braner ◽  
Stefan Zielonka ◽  
Julius Grzeschik ◽  
Simon Krah ◽  
Sebastian Lieb ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Esters ◽  
Novozym 435 ◽  
Hydrolysis Of ◽  
Acid Esters

scholarly journals Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis

2016 ◽  
Vol 11 (2) ◽  
pp. 54-66 ◽  
Author(s):  
Nur Izyan Wan Azelee ◽  
Jamaliah Md Jahim ◽  
Ahmad Fauzi Ismail ◽  
Siti Fatimah Zaharah Mohamad Fuzi ◽  
Roshanida A. Rahman ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reaction Conditions ◽  
Hemicellulose Hydrolysis ◽  
Hydrolysis Of

scholarly journals Scientific support for innovative development of wheat bread long-term storage production

2020 ◽  
Vol 176 ◽  
pp. 03021
Author(s):  
Natalia Zavorokhina ◽  
Natalia Pankratyeva ◽  
Nadezhda Goncharova
Keyword(s):  
Shelf Life ◽  
Fatty Acid Esters ◽  
Wheat Bread ◽  
Innovative Development ◽  
Long Term Storage ◽  
High Degree ◽  
First Time ◽  
Term Storage ◽  
Acid Esters

Nowadays in the context of innovative development the bread intended for long-term storage is necessary in hard-to-reach areas of Sverdlovsk region, participants of expeditions, workers of logging, geological parties, etc. Taking into account the constant demand for such products, it is necessary to develop new approaches to their creation.The aim of the study was the development of long-term storage bread from wheat flour, preserving consumer characteristics throughout the shelf life.The synergism of betulin,nisin and ascorbic acid was studied for the first time when they are used together to inhibit potato bread disease (hereinafter CBH). It is proved that the use of additives, including 0.015% nisin together with 0.005% betulin to the mass of flour increases the shelf life of wheat bread up to 7 days since betulin inhibits CBH, nisin inhibits the development of mold under pH conditions in the range of 5.5-6.5.For the first time, a technique is proposed for producing betulin nanosuspension with a high degree of dispersion. The effect of betulin nanosuspension with emulsifiers of calcium lactylate, polyglycerol fatty acid esters, lecithin on the inhibition of CBH and an increase in the shelf life of wheat bread was studied.

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