Novel Molecular Markers for Late Phases of the Growth Cycle of Arabidopsis thaliana Cell-Suspension Cultures Are Expressed during Organ Senescence

Abstract Four plant bioregulators were tested for their effects on production of valepotriates in Valeriana wallichii and Fedia cornucopiae cell suspension cultures. Concentrations of more than 10 ppm reduced valepotriate yield. At lower concentrations production was increased. For optimal activity, bioregulators had to be applied during early exponential growth, up to day 8 of the growth cycle. At equimolar concentrations dim ethylm orpholinium bromide (4 ppm) and dimethylpiperidinium chloride (3 ppm) significantly im proved total valepotriates in V. wallichii (up to 23%) and in F cornucupiae (up to 50% ) 2-(3,4-dichlorophenoxy ) - triethylamine (6 ppm ) and 2-(3,5-diisopropylphenoxy)-triethylam ine (6.4 ppm) increased valepotriate production in both cell cultures up to 40%. With dimethylpiperidinium chloride and dimethylmorpholinium bromide the ratio of m onoene to diene valepotriates in both cell systems was significantly shifted to the m onoene com pounds. A general use of these bioregulators to increase production of terpenoid secondary m etabolites in plant tissue cultures is indicated.

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Sterol and sesquiterpenoid biosynthesis during a growth cycle of tobacco cell suspension cultures

Plant Cell Reports ◽

10.1007/bf00735777 ◽

1989 ◽

Vol 8 (1) ◽

pp. 48-52 ◽

Cited By ~ 23

Author(s):

Joseph Chappell ◽

Carol Von Lanken ◽

Urs Vögeli ◽

Prashant Bhatt

Keyword(s):

Cell Suspension ◽

Cell Suspension Cultures ◽

Growth Cycle ◽

Suspension Cultures ◽

Tobacco Cell ◽

Tobacco Cell Suspension

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Metabolic Fingerprinting and Profiling of Arabidopsis thaliana Leaf and its Cultured Cells T87 by GC/MS

Zeitschrift für Naturforschung C ◽

10.1515/znc-2006-3-419 ◽

2006 ◽

Vol 61 (3-4) ◽

pp. 267-272 ◽

Cited By ~ 14

Author(s):

Eiichiro Fukusaki ◽

Kanokwan Jumtee ◽

Takeshi Bamba ◽

Takehiro Yamaji ◽

Akio Kobayashi

Keyword(s):

Arabidopsis Thaliana ◽

Cell Suspension ◽

Cultured Cells ◽

Cell Suspension Cultures ◽

Metabolite Profile ◽

Culture Cell ◽

Suspension Cultures ◽

Primary Metabolite ◽

Primary Metabolites ◽

Suspension Culture Cell

Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites extracted from the leaves of Arabidopsis thaliana and cultured cells from an Arabidopsis suspension culture (cell line T87) was performed. The results suggest that although cell suspension cultures and Arabidopsis leaves showed similarities in the common primary metabolite profile, nonetheless, moderate differences in quantitative profile were revealed.

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