Subcellular localization of maize cytokinin dehydrogenases using heterologous expression in Arabidopsis thaliana Ler cell suspension cultures

2014 ◽  
Vol 31 ◽  
pp. S184-S185
Author(s):  
Patricie Johnová ◽  
David Zalabák ◽  
Ondřej Plíhal ◽  
Olga Šamajová ◽  
Petr Galuszka
Keyword(s):  
Arabidopsis Thaliana ◽  
Subcellular Localization ◽  
Heterologous Expression ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Metabolic Fingerprinting and Profiling of Arabidopsis thaliana Leaf and its Cultured Cells T87 by GC/MS

2006 ◽  
Vol 61 (3-4) ◽  
pp. 267-272 ◽  
Author(s):  
Eiichiro Fukusaki ◽  
Kanokwan Jumtee ◽  
Takeshi Bamba ◽  
Takehiro Yamaji ◽  
Akio Kobayashi
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Metabolite Profile ◽  
Culture Cell ◽  
Suspension Cultures ◽  
Primary Metabolite ◽  
Primary Metabolites ◽  
Suspension Culture Cell

Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites extracted from the leaves of Arabidopsis thaliana and cultured cells from an Arabidopsis suspension culture (cell line T87) was performed. The results suggest that although cell suspension cultures and Arabidopsis leaves showed similarities in the common primary metabolite profile, nonetheless, moderate differences in quantitative profile were revealed.

Network flux analysis: Impact of 13C-substrates on metabolism in Arabidopsis thaliana cell suspension cultures

2007 ◽  
Vol 68 (16-18) ◽  
pp. 2176-2188 ◽  
Author(s):  
Nicholas J. Kruger ◽  
Joanna E. Huddleston ◽  
Pascaline Le Lay ◽  
Nicholas D. Brown ◽  
R. George Ratcliffe
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Flux Analysis

Changes in functional properties of mitochondria during growth cycle of Arabidopsis thaliana cell suspension cultures

1998 ◽  
Vol 36 (5) ◽  
pp. 347-356 ◽  
Author(s):  
Jacques Davy de Virville ◽  
Marie-Françoise Alin ◽  
Yvonne Aaron ◽  
René Rémy ◽  
Thérése Guillot-Salomon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Functional Properties ◽  
Cell Suspension Cultures ◽  
Growth Cycle ◽  
Suspension Cultures

scholarly journals Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts

2014 ◽  
Vol 65 (12) ◽  
pp. 3081-3095 ◽  
Author(s):  
Jorge Gutiérrez ◽  
Sergio González-Pérez ◽  
Francisco García-García ◽  
Cara T. Daly ◽  
Óscar Lorenzo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Cell Suspension ◽  
Rose Bengal ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures

1995 ◽  
Vol 27 (4) ◽  
pp. 681-692 ◽  
Author(s):  
Kay Teja Junghanns ◽  
Richard Edward Kneusel ◽  
Alfred Baumert ◽  
Walter Maier ◽  
Detlef Gr�ger ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Cell Suspension ◽  
Molecular Cloning ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
L Cell

Isoflavone And Pterocarpan Malonylglucosides And ß - L,3 - Glucan - And Chitin-Hydrolases Are Vacuolar Constituents In Chickpea (Cicer Arietinum L.)

1992 ◽  
Vol 47 (11-12) ◽  
pp. 815-822 ◽  
Author(s):  
Ulrike Mackenbrock ◽  
Ralph Vogelsang ◽  
Wolfgang Barz
Keyword(s):  
Subcellular Localization ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Hplc Analysis ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Vacuolar Membrane ◽  
Marker Enzymes ◽  
Cicer Arietinum L ◽  
Isoelectric Points

Cell suspension cultures of chickpea (Cicer arietinum L.) were used to prepare protoplasts and vacuoles. The vacuolar preparation revealed only slight contaminations of cytoplasmic marker enzymes. HPLC analysis of the vacuolar extract showed that the malonylglucosides of isoflavones, isoflavanones and pterocarpans are exclusively located in the vacuole. Experiments designed to determine the subcellular localization of the isoflavone malonylglucoside: malonylesterase suggest an association of this enzyme with the vacuolar membrane. Finally, a β-l,3-glucanase and a chitinase with basic isoelectric points were also found to be localized in the chickpea vacuoles

scholarly journals Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for The Overexpression of The COI1 Receptor

BIO-PROTOCOL ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eva Karina Diaz Sanchez ◽  
Jose O’Brien ◽  
Imma Pérez-Salamó ◽  
Jovaras Krasauskas ◽  
Moritz Bömer ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Stable Transformation
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