Copper Extrusion after Accumulation during Growth of Copper-Tolerant Yeast Yarrowia lipolytica

2007 ◽  
Vol 62 (1-2) ◽  
pp. 77-82 ◽  
Author(s):  
Hiroyasu Ito ◽  
Masahiro Inouhe ◽  
Hiroshi Tohoyama ◽  
Masanori Joho
Keyword(s):  
Yarrowia Lipolytica ◽  
Sulfonic Acid ◽  
Heavy Metal Ions ◽  
Culture Medium ◽  
Logarithmic Phase ◽  
Late Logarithmic Phase ◽  
High Concentrations ◽  
Limited Culture ◽  
Yeast Yarrowia Lipolytica ◽  
Copper Tolerant

The Cu2+-tolerant yeast Yarrowia lipolytica accumulated Cu2+ until the late logarithmic phase. Thereafter, Cu2+ was temperature-dependently extruded into phosphate-limited culture medium containing high concentrations of heavy metal ions but not into 10 mm 2-(Nmorpholino) ethane sulfonic acid (MES) buffer (pH 6.0). Peptone in the culture medium played an important role in the extrusion, which proceeded even when peptone was substituted with cysteine or histidine, but not with any other amino acid tested.

TEM study of a biofilm on copper corrosion

1996 ◽  
Vol 54 ◽  
pp. 220-221
Author(s):  
W. A. Chiou ◽  
N. Kohyama ◽  
B. Little ◽  
P. Wagner ◽  
M. Meshii
Keyword(s):  
Marine Bacterium ◽  
Culture Medium ◽  
Copper Alloys ◽  
Pure Copper ◽  
Localized Corrosion ◽  
Natural Seawater ◽  
Copper Corrosion ◽  
New Approach ◽  
The Past ◽  
Copper Tolerant

The corrosion of copper and copper alloys in a marine environment is of great concern because of their widespread use in heat exchangers and steam condensers in which natural seawater is the coolant. It has become increasingly evident that microorganisms play an important role in the corrosion of a number of metals and alloys under a variety of environments. For the past 15 years the use of SEM has proven to be useful in studying biofilms and spatial relationships between bacteria and localized corrosion of metals. Little information, however, has been obtained using TEM capitalizing on its higher spacial resolution and the transmission observation of interfaces. The research presented herein is the first step of this new approach in studying the corrosion with biological influence in pure copper.Commercially produced copper (Cu, 99%) foils of approximately 120 μm thick exposed to a copper-tolerant marine bacterium, Oceanospirillum, and an abiotic culture medium were subsampled (1 cm × 1 cm) for this study along with unexposed control samples.

scholarly journals THE ANTIGENIC POTENCY OF EPIDEMIC INFLUENZA VIRUS FOLLOWING INACTIVATION BY ULTRAVIOLET RADIATION

1940 ◽  
Vol 72 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Jonas E. Salk ◽  
G. I. Lavin ◽  
Thomas Francis
Keyword(s):  
Tissue Culture ◽  
Influenza Virus ◽  
Ultraviolet Radiation ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Intraperitoneal Route ◽  
Active Virus ◽  
High Concentrations

A study of the antigenic potency of influenza virus inactivated by ultraviolet radiation has been made. Virus so inactivated is still capable of functioning as an immunizing agent when given to mice by the intraperitoneal route. In high concentrations inactivated virus appears to be nearly as effective as active virus but when quantitative comparisons of the immunity induced by different dilutions are made, it is seen that a hundredfold loss in immunizing capacity occurs during inactivation. Virus in suspensions prepared from the lungs of infected mice is inactivated more rapidly than virus in tissue culture medium. A standard for the comparison of vaccines of epidemic influenza virus is proposed.

scholarly journals Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 32 ◽  
Author(s):  
Mugdha Apte ◽  
Devashree Sambre ◽  
Shital Gaikawad ◽  
Swanand Joshi ◽  
Ashok Bankar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

scholarly journals Replication of DNA containing 5-bromouracil can be mutagenic in Syrian hamster cells.

1984 ◽  
Vol 4 (11) ◽  
pp. 2449-2454 ◽  
Author(s):  
E R Kaufman
Keyword(s):  
Syrian Hamster ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Cells In Culture ◽  
Thymidine Analog ◽  
High Concentrations

A new protocol for inducing mutations in mammalian cells in culture by exposure to the thymidine analog 5-bromodeoxyuridine (BrdUrd) was established. This protocol, called "DNA-dependent" mutagenesis, involved the incorporation of BrdUrd into DNA under nonmutagenic conditions and the subsequent replication of the 5-bromouracil (BrUra)-containing DNA under mutagenic conditions but with no BrdUrd present in the culture medium. The mutagenic conditions were induced by allowing BrUra-containing DNA to replicate in the presence of high concentrations of thymidine. This generated high intracellular levels of dTTP and dGTP, causing nucleotide pool imbalance. The mutagenesis induced by this protocol was found to correlate with the level of BrUra substituted for thymine in DNA.

scholarly journals Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sivamoke Dissook ◽  
Tomohisa Kuzuyama ◽  
Yuri Nishimoto ◽  
Shigeru Kitani ◽  
Sastia Putri ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Authentic Standard ◽  
Chemical Inhibition ◽  
Chromatographic Peaks ◽  
Methyl Erythritol Phosphate ◽  
Limiting Condition ◽  
Yeast Yarrowia Lipolytica

AbstractMethyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.

scholarly journals Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Thomas Beneyton ◽  
Stéphane Thomas ◽  
Andrew D. Griffiths ◽  
Jean-Marc Nicaud ◽  
Antoine Drevelle ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Throughput ◽  
High Throughput Screening ◽  
Yeast Yarrowia Lipolytica

scholarly journals pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor

2018 ◽  
Vol 365 (16) ◽  
Author(s):  
Doria Naila Bouchedja ◽  
Sabine Danthine ◽  
Tambi Kar ◽  
Patrick Fickers ◽  
Hosni Sassi ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Yarrowia Lipolytica ◽  
Strong Impact ◽  
Ph Level ◽  
Yeast Yarrowia Lipolytica

Effectiveness of Commercial Disinfectants for Inactivating Hepatitis A Virus on Agri-Food Surfaces

2003 ◽  
Vol 66 (1) ◽  
pp. 115-119 ◽  
Author(s):  
J. JEAN ◽  
J.-F. VACHON ◽  
O. MORONI ◽  
A. DARVEAU ◽  
I. KUKAVICA-IBRULJ ◽  
...  
Keyword(s):  
Active Ingredient ◽  
Quaternary Ammonium ◽  
High Density Polyethylene ◽  
Contact Time ◽  
Sulfonic Acid ◽  
Hepatitis A ◽  
Incubation Temperature ◽  
Hepatitis A Virus ◽  
Dodecylbenzene Sulfonic Acid ◽  
High Concentrations

Six commercial disinfectants were tested for their efficacy in inactivating hepatitis A virus in solution or attached to agri-food surfaces. Disinfectant I contains 10% quaternary ammonium plus 5% glutaraldehyde;disinfectant II contains 12% sodium hypochlorite; disinfectant III contains 2.9% dodecylbenzene sulfonic acid plus 16% phosphoric acid; disinfectant IV contains 10% quaternary ammonium; disinfectant V contains 2% iodide; and disinfectant VI contains 2% stabilized chlorine dioxide. Among these, disinfectants I and II were shown to be the most effective in inactivating hepatitis A virus in solution. The efficacy of these disinfectants was further tested against hepatitis A virus attached to common agri-food surfaces, including polyvinyl chlorine, high-density polyethylene, aluminum, stainless steel, and copper. Disinfectant II was shown to be the most effective, with a maximum inactivation level of about 3 log10. The inactivation efficacy was shown to be affected by the concentration of the active ingredient, the contact time between the disinfectant and the contaminated surfaces, and the incubation temperature. In general, hepatitis A virus was shown to be highly resistant to most disinfectants tested, and high concentrations of active ingredient were needed to achieve acceptable inactivation levels.

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