scholarly journals Subunit Heterogeneity of Cytoplasmic Dynein: Differential Expression of 14 kDa Dynein Light Chains in Rat Hippocampus

2001 ◽  
Vol 21 (15) ◽  
pp. 5501-5512 ◽  
Author(s):  
Jen-Zen Chuang ◽  
Teresa A. Milner ◽  
Ching-Hwa Sung
Keyword(s):  
Differential Expression ◽  
Cytoplasmic Dynein ◽  
Rat Hippocampus ◽  
Light Chains ◽  
Dynein Light Chains

scholarly journals Interactions of LC8 with N-Terminal Segments of the Intermediate Chain of Cytoplasmic Dynein

2003 ◽  
Vol 3 ◽  
pp. 647-654 ◽  
Author(s):  
Afua Nyarko ◽  
Michael Hare ◽  
Moses Makokha ◽  
Elisar Barbar
Keyword(s):  
Limited Proteolysis ◽  
Coiled Coil ◽  
Cytoplasmic Dynein ◽  
Light Chains ◽  
Ordered Structure ◽  
Dynein Motor ◽  
Dynein Light Chains ◽  
Significant Conformational Change ◽  
Intermediate Chain

LC8, a highly conserved 10-kDa light chain, and IC74, a 74-kDa intermediate chain, are presumed to promote the assembly of the cytoplasmic dynein motor protein complex and to be engaged in the controlled binding and release of cargo. The interactions of LC8 from Drosophila melanogaster with constructs of IC74 were characterized in vitro by affinity methods, limited proteolysis, and circular dichroism spectroscopy. Previously, we have performed limited proteolysis on the N-terminal domain of IC74, IC(1-289), when free and when bound to dynein light chains LC8 and Tctex-1[1]. We have also shown that upon addition of LC8, IC(1-289) undergoes a significant conformational change from a largely unfolded to a more ordered structure. The purpose of the work presented here is to determine whether residues 1-30 in IC74, predicted to be in a coiled coil, are involved in the stabilization of the protein upon binding to LC8. Constructs of IC74, IC(1-143), and IC(30-143) that include the LC8 binding site but with and without the first 30 residues were prepared, and their binding and protection patterns were compared to our previous results for IC(1-289). The results suggest that coiled coil residues 1-30 are not responsible for the increase in structure we observe when IC(1-289) binds to LC8.

scholarly journals Interactions of Cytoplasmic Dynein Light Chains Tctex-1 and LC8 with the Intermediate Chain IC74†

Biochemistry ◽  
2002 ◽  
Vol 41 (13) ◽  
pp. 4302-4311 ◽  
Author(s):  
Moses Makokha ◽  
Michael Hare ◽  
Mingang Li ◽  
Thomas Hays ◽  
Elisar Barbar
Keyword(s):  
Cytoplasmic Dynein ◽  
Light Chains ◽  
Dynein Light Chains ◽  
Intermediate Chain

scholarly journals Somatic CRISPR–Cas9-induced mutations reveal roles of embryonically essential dynein chains in Caenorhabditis elegans cilia

2015 ◽  
Vol 208 (6) ◽  
pp. 683-692 ◽  
Author(s):  
Wenjing Li ◽  
Peishan Yi ◽  
Guangshuo Ou
Keyword(s):  
Caenorhabditis Elegans ◽  
Intraflagellar Transport ◽  
Cytoplasmic Dynein ◽  
Regulatory Mechanisms ◽  
Light Chains ◽  
Induced Mutations ◽  
Functional Studies ◽  
Cilium Formation ◽  
Dynein Intermediate Chain ◽  
Intermediate Chain

Cilium formation and maintenance require intraflagellar transport (IFT). Although much is known about kinesin-2–driven anterograde IFT, the composition and regulation of retrograde IFT-specific dynein remain elusive. Components of cytoplasmic dynein may participate in IFT; however, their essential roles in cell division preclude functional studies in postmitotic cilia. Here, we report that inducible expression of the clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 system in Caenorhabditis elegans generated conditional mutations in IFT motors and particles, recapitulating ciliary defects in their null mutants. Using this method to bypass the embryonic requirement, we show the following: the dynein intermediate chain, light chain LC8, and lissencephaly-1 regulate retrograde IFT; the dynein light intermediate chain functions in dendrites and indirectly contributes to ciliogenesis; and the Tctex and Roadblock light chains are dispensable for cilium assembly. Furthermore, we demonstrate that these components undergo biphasic IFT with distinct transport frequencies and turnaround behaviors. Together, our results suggest that IFT–dynein and cytoplasmic dynein have unique compositions but also share components and regulatory mechanisms.

Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus

2004 ◽  
Vol 122 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Elena Pompili ◽  
Stefania Lucia Nori ◽  
Maria Concetta Geloso ◽  
Enrico Guadagni ◽  
Valentina Corvino ◽  
...  
Keyword(s):  
Differential Expression ◽  
Reactive Astrocytes ◽  
Rat Hippocampus

Characterisation of a family of Schistosoma japonicum proteins related to dynein light chains

1999 ◽  
Vol 1432 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Wen Yang ◽  
Malcolm K. Jones ◽  
Jinjiang Fan ◽  
Sheree R. Hughes-Stamm ◽  
Donald P. McManus
Keyword(s):  
Schistosoma Japonicum ◽  
Light Chains ◽  
Dynein Light Chains

Cytoplasmic Dynein Contains a Family of Differentially Expressed Light Chains†

Biochemistry ◽  
1998 ◽  
Vol 37 (43) ◽  
pp. 15033-15041 ◽  
Author(s):  
Stephen M. King ◽  
Elisa Barbarese ◽  
James F. Dillman ◽  
Sharon E. Benashski ◽  
Khanh T. Do ◽  
...  
Keyword(s):  
Cytoplasmic Dynein ◽  
Differentially Expressed ◽  
Light Chains

The protein encoded by the germ plasm RNA Germes associates with dynein light chains and functions in Xenopus germline development

2007 ◽  
Vol 75 (6) ◽  
pp. 546-558 ◽  
Author(s):  
Lyubov A. Berekelya ◽  
Alexander A. Mikryukov ◽  
Natalia N. Luchinskaya ◽  
Maxim B. Ponomarev ◽  
Hugh R. Woodland ◽  
...  
Keyword(s):  
Germ Plasm ◽  
Light Chains ◽  
Germline Development ◽  
Dynein Light Chains
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