scholarly journals High Performance Liquid Chromatographic Analysis of Rutin in Allium Species from Bosnia and Herzegovina

2020 ◽  
Vol 69 (11-12) ◽  
pp. 647-651
Author(s):  
Mirsada Salihović ◽  
Emin Sofić
Keyword(s):  
Bosnia And Herzegovina ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Allium Species ◽  
Beneficial Effects ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Harmful Effects ◽  
Capillary Walls

Rutin is quercetin heteroside widely present in plants that exhibit many health-beneficial effects, such as strengthening the capillary walls, reducing the harmful effects of LDL cholesterol, and reducing the risk of cardiovascular diseases. Rutin was determined from three <i>Allium</i> species (<i>A. cepa</i>, <i>A. sativum</i>, and <i>A. ursinum</i>) collected in Bosnia and Herzegovina by the HPLC method associated with electrochemical detection. The analysis was performed from methanol extracts of bulb and leaf of garlic, bulb and leaf of onion, and leaves of wood garlic. Rutin was present in all of the examined samples. The highest rutin content was found in garlic leaves (0.78 ± 0.09 mg g<sup>–1</sup>), and the lowest in onion bulbs (0.04 ± 0.10 mg g<sup>–1</sup>). The contents of rutin were higher in leaf samples, suggesting that leaves of onion and garlic are recommended as a better natural source of this glycoside.

High Performance Liquid Chromatographic Analysis of Rotenone Formulations: Collaborative Study

1983 ◽  
Vol 66 (3) ◽  
pp. 796-800
Author(s):  
Rodney J Bushway ◽  
◽  
M J Bennett ◽  
W R Betker ◽  
R Bolduc ◽  
...  
Keyword(s):  
High Performance ◽  
Collaborative Study ◽  
High Performance Liquid Chromatographic ◽  
Peak Height ◽  
Hplc Method ◽  
Reverse Phase Hplc ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Analysis ◽  
Infrared Method ◽  
Liquid Chromatographic

Abstract A collaborative study was conducted on a high performance liquid chromatographic (HPLC) method for determining rotenone in formulations. One liquid and 4 dust samples, containing other rotenoids and pesticides, with rotenone guarantees ranging from 0.75% to 20% were analyzed by 16 laboratories. Peak height measurements were used by 14 laboratories while 8 laboratories used integrator area measurements. Samples were extracted on a rotary shaker for 1% h with dioxane. An aliquot was analyzed using reverse phase HPLC and UV detection at 280 nm. Coefficients of variation ranged from 2.47 to 3.19% for peak height determinations and from 2.56 to 6.00% for peak area determinations. One collaborator analyzed the samples by the official infrared method for a comparison. The HPLC method has been adopted official first action.

scholarly journals A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations

2011 ◽  
Vol 8 (s1) ◽  
pp. S41-S46
Author(s):  
Prafulla Kumar Sahu ◽  
M. Mathrusri Annapurna ◽  
Dillipkumar Sahoo
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Aqueous Acetic Acid ◽  
Linear Calibration ◽  
Acceptance Range ◽  
Liquid Chromatographic

This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines.

Sign in / Sign up

Export Citation Format

Share Document