scholarly journals Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java

2018 ◽  
Vol 10 (2) ◽  
pp. 320-325
Author(s):  
Rokhmani Rokhmani ◽  
Endang Ariyani Setyawati ◽  
Daniel Joko Wahyono
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Survey Method ◽  
Rrna Gene ◽  
Morphological Variations ◽  
Central Java ◽  
Reverse Primer ◽  
Partial 18S ◽  
Forward Primer

Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body’s morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 ‘-AAC CTG GTT GAT CCT GCC ATG-3’) and Reverse primer (5 ‘-TGA TCC TTC TGC AGG TTC ACC TAC-3’) which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java

Molecular characterisation of two known species of Paratylenchus Micoletzky, 1922 from Iran with notes on the validity of Paratylenchus audriellus Brown, 1959

Nematology ◽  
2016 ◽  
Vol 18 (5) ◽  
pp. 591-604 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Pablo Castillo ◽  
Mozhgan Ziaie Bidhendi ◽  
Juan E. Palomares-Rius
Keyword(s):  
18S Rrna ◽  
First Record ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Female Tail ◽  
Valid Taxon ◽  
Two Populations ◽  
Partial 18S

During a survey on pin nematodes in western Iran, two populations of Paratylenchus audriellus and Paratylenchus tenuicaudatus were collected and subsequently analysed morphologically and molecularly. Paratylenchus audriellus is characterised by the long stylet (48-61 μm) and the typical female tail with a characteristic claw-like process with sharply pointed terminus. To our knowledge, the Iranian population of P. tenuicaudatus is the first record from Iran. The molecular characterisation of P. audriellus nematodes using the D2-D3 of 28S rRNA and the partial 18S rRNA gene sequences revealed that this species is clearly separated from P. straeleni and should be considered as a valid taxon.

scholarly journals Babesia microti in Rodents from Different Habitats of Lithuania

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1707
Author(s):  
Dalytė Mardosaitė-Busaitienė ◽  
Jana Radzijevskaja ◽  
Linas Balčiauskas ◽  
Algimantas Paulauskas
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Reservoir Host ◽  
Clethrionomys Glareolus ◽  
Babesia Microti ◽  
Rrna Gene ◽  
Apodemus Flavicollis ◽  
Study Sites ◽  
First Time ◽  
Partial 18S

Babesia microti (Aconoidasida: Piroplasmida) (Franca, 1910) is an emerging tick-borne parasite with rodents serving as the considered reservoir host. However, the distribution of B. microti in Europe is insufficiently characterized. Based on the sample of 1180 rodents from 19 study sites in Lithuania, the objectives of this study were: (1) to investigate the presence of Babesia parasites in eight species of rodents, (2) to determine the prevalence of Babesia parasites in rodents from different habitats, and (3) to characterize the detected Babesia strains using partial sequencing of the 18S rRNR gene. Babesia DNA was detected in 2.8% rodents. The highest prevalence of Babesia was found in Microtus oeconomus (14.5%) and Microtus agrestis (7.1%) followed by Clethrionomys glareolus (2.3%), Apodemus flavicollis (2.2%) and Micromys minutus (1.3%). In M. minutus, Babesia was identified for the first time. The prevalence of Babesia-infected rodents was higher in the meadow (5.67%) than in the ecotone (1.69%) and forest (0.31%) habitats. The sequence analysis of the partial 18S rRNA gene reveals that Babesia isolates derived from rodents were 99–100% identical to human pathogenic B. microti ‘Jena/Germany’ strain.

scholarly journals Amoebic gill disease outbreak in marine fish cultured in Korea

2017 ◽  
Vol 29 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Wi-Sik Kim ◽  
Kyoung-Hui Kong ◽  
Jong-Oh Kim ◽  
Sung-Ju Jung ◽  
Jeong-Ho Kim ◽  
...  
Keyword(s):  
Fish Species ◽  
18S Rrna ◽  
Vibrio Anguillarum ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Oplegnathus Fasciatus ◽  
Rock Bream ◽  
Black Seabream ◽  
Gill Disease ◽  
Partial 18S

In 2015, 6.7–60% mortality was observed in black seabream ( Acanthopagrus schlegelii), rock bream ( Oplegnathus fasciatus), and gray mullet ( Mugil cephalus) farmed in the southern coast of Korea. On examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. Some rock bream and gray mullet were coinfected with bacteria ( Pseudomonas anguilliseptica, Vibrio tapetis, or Vibrio anguillarum). Histologic examination revealed extensive hyperplastic epithelium and lamellar fusion in the gills. Numerous amoebae were seen between gill filaments. The amoebae collected from the 3 fish species had specific 630 bp of a partial 18S rRNA gene fragment for Neoparamoeba perurans. Phylogenetic analysis based on partial 18S rRNA gene nucleotide sequences revealed that these Korean amoeba isolates belonged to the N. perurans group. Based on our results, black seabream, rock bream, and gray mullet were added as new hosts for N. perurans.

scholarly journals Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java

2021 ◽  
Vol 3 (1) ◽  
pp. 26
Author(s):  
Rokhmani Rokhmani ◽  
Daniel Joko Wahyono ◽  
Lilis Mulyani
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Fish Growth ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Gene Detection ◽  
Central Java ◽  
Polymerase Chain ◽  
18S Rrna Genes

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.

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