scholarly journals Babesia microti in Rodents from Different Habitats of Lithuania

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1707
Author(s):  
Dalytė Mardosaitė-Busaitienė ◽  
Jana Radzijevskaja ◽  
Linas Balčiauskas ◽  
Algimantas Paulauskas
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Reservoir Host ◽  
Clethrionomys Glareolus ◽  
Babesia Microti ◽  
Rrna Gene ◽  
Apodemus Flavicollis ◽  
Study Sites ◽  
First Time ◽  
Partial 18S

Babesia microti (Aconoidasida: Piroplasmida) (Franca, 1910) is an emerging tick-borne parasite with rodents serving as the considered reservoir host. However, the distribution of B. microti in Europe is insufficiently characterized. Based on the sample of 1180 rodents from 19 study sites in Lithuania, the objectives of this study were: (1) to investigate the presence of Babesia parasites in eight species of rodents, (2) to determine the prevalence of Babesia parasites in rodents from different habitats, and (3) to characterize the detected Babesia strains using partial sequencing of the 18S rRNR gene. Babesia DNA was detected in 2.8% rodents. The highest prevalence of Babesia was found in Microtus oeconomus (14.5%) and Microtus agrestis (7.1%) followed by Clethrionomys glareolus (2.3%), Apodemus flavicollis (2.2%) and Micromys minutus (1.3%). In M. minutus, Babesia was identified for the first time. The prevalence of Babesia-infected rodents was higher in the meadow (5.67%) than in the ecotone (1.69%) and forest (0.31%) habitats. The sequence analysis of the partial 18S rRNA gene reveals that Babesia isolates derived from rodents were 99–100% identical to human pathogenic B. microti ‘Jena/Germany’ strain.

Molecular characterisation of two known species of Paratylenchus Micoletzky, 1922 from Iran with notes on the validity of Paratylenchus audriellus Brown, 1959

Nematology ◽  
2016 ◽  
Vol 18 (5) ◽  
pp. 591-604 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Pablo Castillo ◽  
Mozhgan Ziaie Bidhendi ◽  
Juan E. Palomares-Rius
Keyword(s):  
18S Rrna ◽  
First Record ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Female Tail ◽  
Valid Taxon ◽  
Two Populations ◽  
Partial 18S

During a survey on pin nematodes in western Iran, two populations of Paratylenchus audriellus and Paratylenchus tenuicaudatus were collected and subsequently analysed morphologically and molecularly. Paratylenchus audriellus is characterised by the long stylet (48-61 μm) and the typical female tail with a characteristic claw-like process with sharply pointed terminus. To our knowledge, the Iranian population of P. tenuicaudatus is the first record from Iran. The molecular characterisation of P. audriellus nematodes using the D2-D3 of 28S rRNA and the partial 18S rRNA gene sequences revealed that this species is clearly separated from P. straeleni and should be considered as a valid taxon.

Morphological and molecular characterisation of Caloosia longicaudata (Loos, 1948) Siddiqi & Goodey, 1963 (Nematoda: Caloosiidae) from Maui, the Hawaiian Islands with notes on some species of the genus

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 381-393 ◽  
Author(s):  
Esther van den Berg ◽  
Sergei Subbotin ◽  
Louwrens Tiedt
Keyword(s):  
18S Rrna ◽  
Hawaiian Islands ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Diagnostic Pcr ◽  
Rflp Profile ◽  
First Time ◽  
Partial 18S ◽  
Scanning Electron

AbstractCaloosia longicaudata is described from Maui, the Hawaiian Islands, for the first time and both sexes are characterised morphologically using light and scanning electron microscopy. Molecular characterisation of C. longicaudata using the D2-D3 domain of 28S rRNA, partial 18S rRNA and ITS rRNA gene sequences is also provided. The phylogenetic relationships of this species with other representatives of the suborder Criconematina are presented and discussed. A diagnostic PCR-ITS-RFLP profile for C. longicaudata is given together with an identification table for eight species of Caloosia. Caloosia langola n. comb. is transferred to the genus and C. shorai is synonymised with H. psidii.

scholarly journals Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java

2018 ◽  
Vol 10 (2) ◽  
pp. 320-325
Author(s):  
Rokhmani Rokhmani ◽  
Endang Ariyani Setyawati ◽  
Daniel Joko Wahyono
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Survey Method ◽  
Rrna Gene ◽  
Morphological Variations ◽  
Central Java ◽  
Reverse Primer ◽  
Partial 18S ◽  
Forward Primer

Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body’s morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 ‘-AAC CTG GTT GAT CCT GCC ATG-3’) and Reverse primer (5 ‘-TGA TCC TTC TGC AGG TTC ACC TAC-3’) which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java

scholarly journals Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 311-321 ◽  
Author(s):  
Marija Rajicic ◽  
Tanja Adnadjevic ◽  
Gorana Stamenkovic ◽  
Jelena Blagojevic ◽  
Mladen Vujosevic
Keyword(s):  
18S Rrna ◽  
Target Genes ◽  
Primordial Germ Cells ◽  
18S Rrna Gene ◽  
B Chromosomes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Apodemus Flavicollis ◽  
Protein Coding ◽  
Exon 1

B chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In various species Bs occur with different nucleotide sequences ranging from repetitive to protein coding. In yellow-necked field mice, Apodemus flavicollis Bs are small euchromatic chromosomes and untill now, only few molecular analyses have been conducted. In this study we examined A. flavicollis individuals with different number of Bs for presence of two genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in three Canidae species and one Cervidae species. This gene is a coding receptor critical for proliferation and cell differentiation of hematopoietic, melanoblast and primordial germ cells, and is highly conserved within mammals. While using semiquantitative PCR, we did not notice any difference in the C-KIT band intensity among animals with different number of Bs (0-3). The presence of only one copy of C- KIT gene was confirmed using real time-PCR on genomic DNA of A. flavicollis specimens with different number of Bs. rRNA genes in eukaryotes? genome are organized like units of tandem repeated sequences. The units form distinct clusters on one to several chromosome pairs. rRNA genes were found on Bs in different species including two species of genus Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA gene about three times that of the calibrator 0 B sample. This result can indicate the presence of 18S rRNA gene on Bs, but its confirmation requires the implementation of other methods. Still, we can neither confirm nor deny the existence of pseudogen of tested target genes, or lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our findings are further discussed.

Host specificity of Trypanosoma (Herpetosoma) species: evidence that bank voles (Clethrionomys glareolus) carry only one T. (H.) evotomys 18S rRNA genotype but wood mice (Apodemus sylvaticus) carry at least two polyphyletic parasites

Parasitology ◽  
2002 ◽  
Vol 124 (2) ◽  
pp. 185-190 ◽  
Author(s):  
H. A. NOYES ◽  
P. AMBROSE ◽  
F. BARKER ◽  
M. BEGON ◽  
M. BENNET ◽  
...  
Keyword(s):  
Host Specificity ◽  
18S Rrna ◽  
Pcr Amplification ◽  
Wood Mouse ◽  
18S Rrna Gene ◽  
Apodemus Sylvaticus ◽  
Clethrionomys Glareolus ◽  
Rrna Gene ◽  
Wood Mice ◽  
Bank Voles

The strongest evidence for host specificity of mammalian trypanosomes comes from parasites of the subgenus Trypanosoma (Herpetosoma). Laboratory studies have shown that T. (Herpetosoma) species will not infect an alternative host. However, this has not been demonstrated in wild populations. We screened 560 bank voles (Clethrionomys glareolus) and 148 wood mice (Apodemus sylvaticus) for trypanosomes by PCR amplification of the 18S rRNA gene. In total, 109 (19%) bank voles and 12 (8%) wood mice were infected. A HaeIII restriction site was discovered that could be used to discriminate between T. (H.) evotomys of the bank vole and T. (H.) grosi of the wood mouse. All the parasites in the bank voles were identified as T. (Herpetosoma) evotomys by RFLP-PCR. Out of the 12 wood mouse infections 10 were due to T. grosi. Two of the wood mice were infected with parasites with a novel genotype that was most similar to those of T. evotomys and T. microti of voles. Fifty-six fleas collected from the rodents were also screened for trypanosomes; 9 were infected with T. evotomys and 1 with T. grosi. One of the fleas infected with T. evotomys was collected from a wood mouse.

scholarly journals Amoebic gill disease outbreak in marine fish cultured in Korea

2017 ◽  
Vol 29 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Wi-Sik Kim ◽  
Kyoung-Hui Kong ◽  
Jong-Oh Kim ◽  
Sung-Ju Jung ◽  
Jeong-Ho Kim ◽  
...  
Keyword(s):  
Fish Species ◽  
18S Rrna ◽  
Vibrio Anguillarum ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Oplegnathus Fasciatus ◽  
Rock Bream ◽  
Black Seabream ◽  
Gill Disease ◽  
Partial 18S

In 2015, 6.7–60% mortality was observed in black seabream ( Acanthopagrus schlegelii), rock bream ( Oplegnathus fasciatus), and gray mullet ( Mugil cephalus) farmed in the southern coast of Korea. On examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. Some rock bream and gray mullet were coinfected with bacteria ( Pseudomonas anguilliseptica, Vibrio tapetis, or Vibrio anguillarum). Histologic examination revealed extensive hyperplastic epithelium and lamellar fusion in the gills. Numerous amoebae were seen between gill filaments. The amoebae collected from the 3 fish species had specific 630 bp of a partial 18S rRNA gene fragment for Neoparamoeba perurans. Phylogenetic analysis based on partial 18S rRNA gene nucleotide sequences revealed that these Korean amoeba isolates belonged to the N. perurans group. Based on our results, black seabream, rock bream, and gray mullet were added as new hosts for N. perurans.

scholarly journals Molecular and morphological characterisation of Sphaeronema alni Turkina & Chizhov, 1986 (Nematoda: Sphaeronematidae) from Spain compared with a topotype population from Russia

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 649-659 ◽  
Author(s):  
Juan Palomares-Rius ◽  
Nicola Vovlas ◽  
Sergei A. Subbotin ◽  
Alberto Troccoli ◽  
Carolina Cantalapiedra-Navarrete ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
18S Rrna ◽  
Castanea Sativa ◽  
Rrna Gene ◽  
28S Rrna ◽  
Rflp Profile ◽  
Basal Clade ◽  
Pcr Rflp ◽  
First Time ◽  
Partial 18S

Abstract The occurrence of a male-less population of Sphaeronema alni parasitising chestnut (Castanea sativa) roots and inducing a stelar syncytium is reported for the first time in Pola de Somiedo (Oviedo province), Spain. Morphometric and molecular characters of the Spanish population matched those of a topotype population from Russia. SEM observations showed swollen females having the first lip annulus wider than the second and appearing as a cap-like, circumoral elevation. The second-stage juveniles, having a single band in the lateral fields, were characterised by a non-annulated dome-shaped lip region derived from the fusion of the oral disc with all the lip sectors and lip annuli, and showing slit-like amphidial apertures and an oval prestoma. The sequences of the D2-D3 expansion segments of 28S rRNA, partial 18S rRNA and ITS rRNA gene for the Spanish and topotype populations of S. alni were congruent and matched those deposited in GenBank for another population from Germany, thereby confirming their conspecificity. A PCR-RFLP profile of D2-D3 of 28S rRNA for identification of this species was also provided. The phylogenetic relationships between S. alni populations and representatives of the suborder Criconematina, as inferred from analysis of partial 18S rRNA and D2-D3 of 28S gene sequences obtained in this and previous studies, indicated that S. alni formed a basal clade on the majority consensus Bayesian phylogenetic trees, standing together with Meloidoderita sp. or alone. These findings provide additional evidence of the need to clarify the position of Sphaeronema within Criconematina and its relationships with representatives of Tylenchulinae.

First detection ofBabesia occultansinHyalommaticks from Tunisia

Parasitology ◽  
2011 ◽  
Vol 138 (5) ◽  
pp. 578-582 ◽  
Author(s):  
A. ROS-GARCÍA ◽  
Y. M'GHIRBI ◽  
A. BOUATTOUR ◽  
A. HURTADO
Keyword(s):  
18S Rrna ◽  
Oligonucleotide Probe ◽  
Epidemiological Studies ◽  
Wide Distribution ◽  
18S Rrna Gene ◽  
Sub Saharan Africa ◽  
Rrna Gene ◽  
Hyalomma Marginatum ◽  
Sub Saharan ◽  
First Time

SUMMARYDescriptions ofBabesia occultanshave previously been restricted to sub-Saharan Africa. Here, we report the finding, for the first time, of this low or non-pathogenic bovineBabesiaspecies in Tunisia, northern Africa.B. occultansDNA was detected by molecular methods inHyalomma marginatumunfed ticks collected in 3 bioclimatic regions of Tunisia. The near-full-length 18S rRNA gene was sequenced and compared with related sequences retrieved from GenBank. Phylogenetic analysis indicated that other sequences deposited asBabesiasp. could also correspond toB. occultans, suggesting that this species may have a wide distribution in Mediterranean and Asiatic regions, and not only in sub-Saharan Africa as previously described. AB. occultans-specific Reverse Line Blot (RLB) oligonucleotide probe was designed for future epidemiological studies that would help to clarify this possibility.

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