scholarly journals BIODEGRADATION OF CHRYSENE BY CONSORTIUM OF BACILLUS CEREUS AND PSEUDOMONAS PUTIDA IN PETROLEUM CONTAMINATED-SOIL ON SLURRY-PHASE BIOREACTOR

2017 ◽  
Vol 6 (2) ◽  
pp. 168-174
Author(s):  
Abubakar Tuhuloula ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti ◽  
Suprapto Suprapto
Keyword(s):  
Crude Oil ◽  
Bacillus Cereus ◽  
Pseudomonas Putida ◽  
Contaminated Soil ◽  
Bacterial Consortium ◽  
Biodegradation Rate ◽  
Bacterial Populations ◽  
Slurry Phase ◽  
Petroleum Contaminated Soil ◽  
C 30

Pollution by chrysene compounds in the polluted soil of petroleum, due to exploration activities, production and disposal of petroleum waste into the environment causing serious damage to the ecosystem environment, became the target of processing with bacteria as a model of remediation of pollution sites. Thus, the study focused on the use of a bacterial consortium to degrade chrysene in petroleum-contaminated soil. The study was conducted by mixing 20:80 (% wt) of contaminated soil with water. The consortium of Bacillus cereus and Pseudomonas putida 10%(v/v) and 15%(v/v) bacteria with ratios; 2:3; 1:1; 3:2 is inserted into the slurry bioreactor. Biodegradation process is run with agitation of 100 rpm and temperature 26<sup>o</sup>C 30<sup>o</sup>C and in aeration. Identification of chrysene using gas chromatographymass spectrometry (GCMS) and bacterial populations with haemycitometer. The initial concentration of chrysene is 24.48 ng/?L. After 49 days remediation period for a 10% (v/v) reduced chrysene bacteria consortium and bacterial populations were 8.68 ng/?L; 7.56 ng/?L; and 8.07 ng/?L; with biodegradation rate is 67.01%; 69.10%; And 64.54%. As for the 15% (v/v) bacteria consortium with the same ratio, chrysene was degraded to 2.60 ng/?L; 1.57 ng/?L; and 2.02 ng/?L and the measured chrysene biodegradation rate was 89.39%; 93.58%; And 91.73%. These findings suggest that the percentage of low crude oil is degraded because of the increasing concentration of crude oil.

scholarly journals Biodegradation of Extractable Petroleum Hydrocarbons by Consortia Bacillus cereus and Pseudomonas putida in Petroleum Contaminated-Soil

2019 ◽  
Vol 19 (2) ◽  
pp. 347 ◽  
Author(s):  
Abubakar Tuhuloula ◽  
Suprapto Suprapto ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Putida ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Oil Concentration ◽  
Petroleum Contaminated Soil ◽  
And Control ◽  
Water Ratio

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.

Bioremediation of Crude Oil-Contaminated Soil Using Slurry-Phase Biological Treatment and Land Farming Techniques

2003 ◽  
Vol 12 (1) ◽  
pp. 85-99 ◽  
Author(s):  
Maria S. Kuyukina ◽  
Irena B. Ivshina ◽  
Marina I. Ritchkova ◽  
James C. Philp ◽  
Colin J. Cunningham ◽  
...  
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Biological Treatment ◽  
Slurry Phase ◽  
Land Farming

Surfactant-enhanced biodegradation of crude oil by mixed bacterial consortium in contaminated soil

2018 ◽  
Vol 25 (15) ◽  
pp. 14437-14446 ◽  
Author(s):  
Ruiwei Xu ◽  
Zhen Zhang ◽  
Liping Wang ◽  
Ningning Yin ◽  
Xiaohui Zhan
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Bacterial Consortium ◽  
Enhanced Biodegradation ◽  
Mixed Bacterial Consortium

scholarly journals Biodegradation Potentials of Bacterial Isolates from Auto-Mechanic Workshops in Oluku, Edo State, Nigeria

2017 ◽  
Vol 1 (2) ◽  
pp. 256-264
Author(s):  
C. E. Oshoma ◽  
E. E. Eze ◽  
S. E. Omonigho
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Bacterial Consortium ◽  
Petroleum Products ◽  
Bacterial Isolates ◽  
E Coli ◽  
Edo State

The frequent discharge of used petroleum products from automobiles has become a major source of concern due to unguided discharge into the soil environment, hence the need for biodegradation of the products. Bacterial species were isolated from contaminated soil in mechanic workshops and screened for their for hydrocarbon degradation potentials using standard microbiological procedures. Physicochemical properties of the contaminated soil were also analysed using standard techniques. The highest and lowest heterotrophic bacterial counts of 2.82 ± 0.16 ×108 and 2.09 ± 0.32 ×108 cfu/g were from the control soil and site 1 respectively. For hydrocarbon utilizing bacterial, Site 2 had the highest load of 8.33 ± 2.55 ×105 while the control had the least 1.35±0.33× 104 cfu/g. The bacterial isolates from the contaminated soil were found to be Corynebacterium kutsceri, Escherichia coli, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Klebsiella oxytoca, Staphylococcus aureus, Pseudomonas aeruginosa and Micrococcus luteus. The highest and lowest in the frequency of occurrence among the isolates were B. subtilis (27.5 %) and E. coli (1.4%) respectively. The screened hydrocarbon utilizing bacterial isolates were C. kutseri, B. subtilis and P. aeruginosa. The ability to degrade crude oil revealed that bacterial consortium had the highest growth profile of 12.90 ×105 while the least was C. kutsceri with values of 8.20 ×105 cfu/g. The consortium bacteria had the highest percentage of hydrocarbon products degradation. The ability of the consortium bacteria to remove a high percentage of crude oil components makes it potentially useful for bioremediation of site highly contaminated with petroleum hydrocarbon.

Screening of Bacterial Consortium Isolated from Oil Contaminated Soil for Its Potential to Degrade Crude Oil

2013 ◽  
Vol 1 (3) ◽  
pp. 242-245 ◽  
Author(s):  
O. P. Abioye ◽  
P. O. Amaefule ◽  
S. A. Aransiola ◽  
E. Stephen
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Bacterial Consortium

scholarly journals A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium

2009 ◽  
Vol 2 (1) ◽  
pp. 127-137 ◽  
Author(s):  
A. Mittal ◽  
P. Singh
Keyword(s):  
Environmental Factors ◽  
Crude Oil ◽  
Feasibility Study ◽  
Contaminated Soil ◽  
Bacterial Consortium ◽  
Microcosm Study ◽  
Spilled Oil ◽  
Indigenous Microflora ◽  
Mixed Bacterial Consortium

A microcosm study evaluating inoculums addition of mixed bacterial consortium to stimulate in-situ bioremediation of crude oil contaminated soil was conducted. In feasibility study, out of five treatments the application of bacterial consortium, nutrients and environmental factors resulted in 79.16% removal of TPH in 60 days, compared to 30.24% removal of TPH carried out by indigenous microflora. Gas chromatograms of original spilled oil and bioremediated oil by the addition of developed consortium shows that Pr/Ph ratio decreased progressively from 2.358 to 1.626. The results showed that the ratio of di/tri aromatics decreased from initial 0.63 to 0.25 with progressive treatment of nutrient addition, as nutrient + tilling, nutrient + tilling + microbial seeding. Similar effect was observed in di/di + tri aromatics ratios which also decreased 0.31 to 0.20 by bioaugmentation only.  Keywords: Hydrocarbons; Pseudomonas; Biodegradation; Gas Chromatogram. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2601                 J. Sci. Res. 2 (1), 127-137 (2010) 

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