INVESTIGATION OF BACTERIOTROPIC PROPERTIES OF JUICE FROM GREEN WHEAT SPROUTS

Juice «vitgrass» made from green wheat sprouts is a valuable biologically active product that has a stimulating effect on the physiological state of a person. Hydroponically grown raw materials for juice production contain proteins, fats, carbohydrates, a large group of vitamins, macro- and microelements, antioxidants, enzymes and other substances. As a part of health programs, the use of this juice is recommended for overweight people to optimize the metabolic processes of the macroorganism. A healthy lifestyle involves reducing the negative impact of environmental factors, including diet correction, on the microbiota of the gastrointestinal tract. The cumulative biological assessment of wheatgrass juice should take into account the results of its effect on the indigenous microflora. Lacto- and bifidobacteria, including industrial strains for the manufacture of probiotic preparations, represent an adequate model for studying the bacteriotropic effect of any plant-based product. The experimental results indicate a pronounced stimulating effect of juice from green wheat sprouts on the growth parameters of probiotic bacterial cultures. Strains of lacto- and bifidobacteria when cultivated under conditions of maximum limitation of nutrients in the composition of the "poor" nutrient medium with the addition of "Vitgrass" juice reacted with an increase in the level of accumulation of cell biomass and a higher activity of acid formation in comparison with the control. The analysis of the results obtained makes it possible to estimate the magnitude of the effects of stimulation in relation to both bacterial cultures as quite comparable, taking into account the known intersstrain differences in growth parameters and their biochemical aspects. This is confirmed by an almost similar increased level of carbohydrate utilization in both variants of culture liquids in the presence of juice. The potential of the positive bacteriotropic activity of "Vitgrass" juice in relation to the representatives of the indigenous microflora can be realized in different ways. It seems expedient to use the product from green wheat sprouts more widely as part of programs for the complex health improvement of the macroorganism, including the prevention and correction of dysbiotic conditions.

Physico-chemical Parameters with Direct Influence on the Dynamism of the Indigenous Microflora of the Traditional Cheese “J’ben Elgafs”

Background: The microbial evolution of the J’ben Elgafs prepared with raw milk from local cows, was studied during the manufacturing and maturating process in order to characterize this variety of cheese from the Algerian terroir.Methods: The microbial activity and physical-chemical parameters were tested during the three dairy seasons of the year. Total, lactics and alteration floras were counted on their selective environments.Result: Lactic germs multiply considerably during the first days and only stabilize towards the end of maturation. The low presence of alteration floras is the result of the continuous modification of the physico-chemical parameters of Aw and pH from one stage to the other of the j’ben production and the respect of good processing practices. These different proportions of variation are induced by the biochemical reactions and microbial interactions that take place responsible for the sequential growth of one microbial group compared to another.

Advantages of the application method for taking a biopsy of the gastric mucosa to assess its functional state and microflora composition in obese children

Relevance. Obesity is one of the most pressing problems of modern Pediatrics.The purpose of the study: to study the morphofunctional state and features of the composition of the stomach microflora in obese children using and comparing various endoscopic methods of biomaterial sampling.Materials and methods. 164 children aged 7 to 17 years were examined. The main group — 85 obese people, the comparison group‑79 children with normal body weight and digestive diseases. Esophagogastroduodenoscopy was performed, followed by pathomorphological, bacterioscopic, and microbiological examination of the biological material. In addition to sampling the biopsy of the antral part of the stomach with a sterile farcept, 40 patients were additionally taken from the wall biological material by obtaining a smear from the mucous membrane with the author’s endoscopic probe.Research result. The frequency of inflammatory changes IN the gastrointestinal tract was high in both groups and did not depend on body weight. In the main group, duodeno-gastric reflux (DGR) (32–38% и 9–11%, p<0.05) and cardia insufficiency (46–54% и 36–46%, p>0.05) were more often diagnosed. With an increase in the degree of obesity, the total number of refluxes increased — from 45–53% at grade I to 64–75% at grade IV, and DGR — from 12–14% to 49–58%, respectively. The most frequent localization of inflammatory changes in children of the main group was the antral (43–50%), less often — the stomach body (26–30%), while in the comparison group the body was more often affected (42–52%) and less often the antral (24–30%). In most children, regardless of the group (56–66% и 51–65%), chronic gastritis of moderate activity was registered. A high degree was significantly more often detected in the comparison group (14–16% and 24–30%, p<0.05), while a weak degree was detected in children of the main group (16–19% and 5–6%, p<0.05). In children of the main group, H. pylori (HP) was more often detected (45–53% и 25–32%, p <0.05), while 1 degree of contamination was more often determined (43–51% и 39–48%), while in the comparison group — 3 degree (15–18% и 24–30%). With increasing degree of obesity increased frequency of contamination from the antrum, HP — from 36–42% when I level up to 60–71% in IV degree of obesity. When using the application in HP+ children of the main group were more often sown opportunistic pathogens in a more diverse species composition and a more pronounced decrease in planting indigenous microflora, and regardless of HP significantly more prevalent growth of E. coli and Candida. In the presence of DGR, independently of the group, a significantly higher growth of fecal flora and a more pronounced decrease in the seeding of indigenous microflora was obtained.Conclusions.1. A high frequency of inflammatory and functional disorders in children, regardless of body weight, was established.2. Motor-evacuation disorders in children with obesity recorded often (63% and 43%). As the degree of obesity increases, their frequency increases. DGR in obese children is recorded significantly more often (38% and 11%, p<0.05).3. Children with obesity have a higher frequency of registering a low degree of activity of chronic gastritis, and children with normal body weight have a high frequency. The degree of activity of the inflammatory process does not depend on the degree of obesity and the duration of the disease.4. HP in obese children is detected 1.5 times more often than in children with normal body weight; the predominance of low (1 degree) contamination is characteristic. With an increase in the degree of obesity, the frequency of HP contamination increases (from 42% at grade I to 70% at grade IV obesity).5. HP-positive children show significantly higher growth of E. Faecium, S. viridans and Streptococcus faecalis and lower seeding of Lactobacterium and Bifidobacterium.6. DGR contributes to a more pronounced decrease in the growth of indigenous microflora, only Streptoco

The state of the intestinal microbiota and antimicrobial protection: obtaining and analyzing information

Despite significant progress in the study of the qualitative and quantitative composition of microflora, there are many controversial issues related not only to diagnosis, but also to the treatment of dysbiotic disorders of the intestine. Dysbiotic disorders, characterized by a decrease in the qualitative and quantitative indicators of obligate and facultative microflora, can have a negative impact on human health. The doctor’s task is to restore the microbiocenosis of the gastrointestinal tract and the density of colonization of indigenous microflora, taking into account modern knowledge on this problem, presented in on-line training seminars.

Specific composition of indigenous microflora (Lactobacillus spp., Bifidobacterium spp., Lactococcus spp.) in farm animals

To maintain a stable composition of the gastrointestinal tract microflora in farm animals it is necessary to use probiotic agents to ensure the full functioning of the digestive, hormonal, and immune systems of the body. Most modern probiotics include lactic acid bacteria and bifidobacteria, which are the most physiologically valuable components of a healthy organism’s an indigenous microflora. The aim of this study was to provide indication and identification from the milk of healthy cows and gastric tract of healthy pigs and calves of the genus bacteria Lactobacillus, Bifidobacterium, and Lactococcus. The objects of research were cultures of microorganisms isolated from cows milk (82), the gastrointestinal tract of cattle (317), and piglets of different age groups (114). Bacteriological studies were carried out on the basis of the veterinary sanitation and parasitology laboratory of the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (Kharkiv) in accordance with current regulatory documents. According to the research of the gastrointestinal tract of clinically healthy calves and piglets isolated and typified to 317 and 114 cultures of microorganisms, the species composition of the microflora (82 bacterial cultures) of the cisternous and parenchymatous milk of clinically healthy cows was determined. A total of 513 isolates of microorganisms were isolated, including: Enterobacter spp. –2 (0,39%), Staphylococcus spp. – 7 (1,37%), Bacillus spp. – 11 (2,14%), Enterococcus spp. – 33 (6.43%), Lactococcus spp. – 75(14,62%), Bifidоbacterium spp. – 170 (33,14%), and Lactobacillus spp. – 215 (41,91%). In the study of the biological properties of isolated microorganisms Lactobacillus spp. (215) established their species identity: L. brevis – 7 (3.26%), L. delbrueckii – 9 (4,19%), L. acidophilus – 21 (9,77%), L. fermentum – 23 (10,69%), L. casei – 57 (26,51%), and L. plantarum – 98 (45.58%). Cultures of Bifidobacterium spp. (170) belong to B. suis – 2 (1,18%), B. breve – 7 (4,12%), B. lactentis – 15 (8,82%), B. bifidum – 21 (12,35%), B. longum – 22 (12,94%), B. infantis – 25 (14,71%), and B. adolescentis – 78 (45,88%). From samples of biological material of farm animals, 75 cultures of the genus Lactococcus spp. were isolated (75) of which Lactococcus lactis is representative. Isolated bacteria Lactobacillus spp., Bifidobacterium spp. and Lactococcus spp. promising when creating innovative probiotic products for farm animals.