TESTOSTERONE METABOLISM IN CULTURED HYPERPLASIA OF THE HUMAN PROSTATE

1974 ◽  
Vol 77 (4) ◽  
pp. 784-793 ◽  
Author(s):  
M. J. McMahon ◽  
A. V. J. Butler ◽  
G. H. Thomas
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Organ Culture ◽  
Culture Medium ◽  
Calf Serum ◽  
Culture Conditions ◽  
Prostatic Hyperplasia ◽  
Human Prostate ◽  
Foetal Calf Serum ◽  
Testosterone Metabolism ◽  
Culture Time

ABSTRACT Slices of human benign prostatic hyperplasia were maintained in organ culture in the presence of [3H]- or [14C]testosterone. Explants concentrated radioactivity from the culture medium, although this effect was depressed by the inclusion of foetal calf serum in the medium. Testosterone was metabolised to products with chromatographic mobilities corresponding to androstanediols, androsterone, dehydroepiandrosterone, and androstanedione. The principal metabolite was identified as 5α-dihydrotestosterone, and small amounts of testosterone and androstenedione were also found. As culture time increased from 1 to 6 days there was a diminution in the proportion of 5α-, and an increase in 17-ketometabolites, indicating a swing to a less physiological pattern of testosterone metabolism by the aging culture. Testosterone metabolism was thought to provide a sensitive index by which to evaluate changes in culture conditions.

Localization of angiotensin-converting enzyme in the human prostate: pathological expression in benign prostatic hyperplasia

2001 ◽  
Vol 195 (5) ◽  
pp. 571-579 ◽  
Author(s):  
Labrini Nassis ◽  
Albert G. Frauman ◽  
Mitsuru Ohishi ◽  
Jialong Zhuo ◽  
David J. Casley ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Angiotensin Converting Enzyme ◽  
Prostatic Hyperplasia ◽  
Human Prostate ◽  
Converting Enzyme

scholarly journals Ghrelin Aggravates Prostate Enlargement in Rats with Testosterone-Induced Benign Prostatic Hyperplasia, Stromal Cell Proliferation, and Smooth Muscle Contraction in Human Prostate Tissues

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Xiaolong Wang ◽  
Yiming Wang ◽  
Christian Gratzke ◽  
Christian Sterr ◽  
Qingfeng Yu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Benign Prostatic Hyperplasia ◽  
Muscle Contraction ◽  
Stromal Cell ◽  
Molecular Mechanisms ◽  
Smooth Muscle Contraction ◽  
Prostatic Hyperplasia ◽  
Human Prostate ◽  
Mrna Levels ◽  
Prostate Enlargement

Epidemiologic studies revealed a context between lower urinary tract symptoms (LUTS) suggestive of benign prostatic hyperplasia (BPH) and metabolic syndrome. However, molecular mechanisms underlying this relationship are largely unknown. Prostate enlargement and increased prostate smooth muscle tone are important factors in the pathophysiology of LUTS suggestive of BPH. In the present study, we studied effects of the metabolic hormone ghrelin on prostate enlargement in rats with experimentally induced BPH, growth of cultured stromal cells from human prostate (WPMY-1), and smooth muscle contraction of human prostate tissues. Ghrelin (20 nmol/kg daily, p.o., 2 weeks) increased prostate size in rats with testosterone-induced BPH. Microarray identified 114 ghrelin-upregulated genes (2-fold or more) in these prostates, with possible roles in growth, smooth muscle contraction, or metabolism. 12 genes were selected for further analyses. In human prostate tissues, mRNA levels of 11 of them correlated positively with ghrelin receptor (GHSR) expression, but only two with the degree of BPH. Accordingly, no correlation was evident between GHSR expression level and BPH in human prostate tissues. In WPMY-1 cells, the GHRS agonist MK0677 upregulated 11 of the selected genes. MK0677 induced proliferation of WPMY-1 cells, shown by EdU assay, colony formation, proliferation markers, flow cytometry, and viability. In myographic measurements, GHSR agonists enhanced contractions of human prostate strips. Together, ghrelin may aggravate prostate enlargement, stromal cell growth, and prostate smooth muscle contraction in BPH. Ghrelin may deteriorate urethral obstruction independently from BPH, qualifying the ghrelin system as an attractive new target to be tested for LUTS treatment in BPH.

Ultrastructural aspects of human prostate in benign prostatic hyperplasia

The Prostate ◽  
1982 ◽  
Vol 3 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Graziella Biagini ◽  
Paola Preda ◽  
Michele Lo Cigno ◽  
Marcello Soli ◽  
Edward Bercovich
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Prostatic Hyperplasia ◽  
Human Prostate

scholarly journals Immunoreactive insulin from mouse brain cells in culture and whole rat brain

1984 ◽  
Vol 218 (1) ◽  
pp. 19-27 ◽  
Author(s):  
N P Birch ◽  
D L Christie ◽  
A G C Renwick
Keyword(s):  
Rat Brain ◽  
Mouse Brain ◽  
Culture Medium ◽  
Calf Serum ◽  
Brain Cells ◽  
Immunoreactive Insulin ◽  
Foetal Calf Serum ◽  
Cells In Culture ◽  
Pancreatic Insulin ◽  
Contrast Microscopy

Foetal mouse brain cells were cultured as described previously [Sotelo, Gibbs, Gajdusek, Toh & Wurth (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 653-657] without added insulin and without foetal calf serum after 12 days in culture. Examination by phase-contrast microscopy showed that these modifications did not appear to affect growth and development of the cells adversely. Silver impregnation of the cultures and indirect immunofluorescence following reaction with tetanus toxin showed that a high proportion of the cells resembled neurones. Analysis of concentrated culture medium by radioimmunoassay and high-pressure liquid chromatography (h.p.l.c.) revealed that the cells produced two main forms of immunoreactive insulin which differed from authentic pancreatic insulin in retention time. Immunoreactive somatostatin was also produced in culture and this was resolved into at least three forms by h.p.l.c. Immunoreactive insulin was also extracted from whole rat brain by using two published procedures. The method of Havrankova, Schmechel, Roth & Brownstein [Proc. Natl. Acad. Sci. U.S.A. (1978) 75, 5737-5741] consistently gave greater yields of insulin than did that of Eng & Yalow [Diabetes (1980) 29, 105-109] and the concentration was about three times that of plasma. The extracted insulin was further characterized by h.p.l.c. in each case and was found to behave like authentic pancreatic insulin. The production of insulin and somatostatin by foetal mouse brain cells in culture suggests that they may be a useful model system for studies of neuropeptide biosynthesis.

scholarly journals Isolation of a serum component that stimulates the spreading of cells in culture

1980 ◽  
Vol 185 (2) ◽  
pp. 349-354 ◽  
Author(s):  
J G Whateley ◽  
P Knox
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Calf Serum ◽  
Culture Conditions ◽  
Foetal Calf Serum ◽  
Spreading Factor ◽  
Cells In Culture ◽  
Serum Component ◽  
Cell Culture Conditions

A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This “spreading” factor is a glycoprotein with a mol. wt. of approx. 62000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 micrograms/ml.

Differential nuclear matrix-intermediate filament expression in human prostate cancer in respect to benign prostatic hyperplasia

1996 ◽  
Vol 109 (1-2) ◽  
pp. 193-198 ◽  
Author(s):  
Ingles Alberti ◽  
Silvio Parodi ◽  
Paola Barboro ◽  
Paola Sanna ◽  
Guido Nicolò ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Nuclear Matrix ◽  
Intermediate Filament ◽  
Prostatic Hyperplasia ◽  
Human Prostate ◽  
Human Prostate Cancer
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