Human growth hormone blunts Na2EDTA-induced hypocalcaemia in hyposomatotrophic children

Abstract. An infusion of disodium ethylenediamine tetraacetate (Na2EDTA) (0.13 mmol/kg for 2 h) was administered to 10 hyposomatotrophic children prior to and after 6 and 12 months of treatment with human growth hormone (hGH). Total and ionized calcium and immunoreactive parathyroid hormone (iPTH) concentrations were determined. Mean basal total and ionized calcium concentrations did not change during the year of treatment with hGH. The nadir concentrations of total and ionized calcium increased progressively during hGH administration and after 12 months were significantly increased over pre-treatment values (total calcium: pre-treatment 1.85 ± 0.32 (sd) mmol/l, + 12 months 2.10 ± 0.15, P < 0.01; ionized calcium: pre-treatment 0.55 ± 0.31 mmol/l, + 12 months 0.78 ± 0.14, P < 0.05). The mean basal concentration of iPTH increased slightly after 12 months of hGH administration (pre-treatment 72 ± 18 pg/ml, + 12 months 106 ± 71, P < 0.05), but Na2EDTA-evoked secretion of iPTH was not significant altered by hGH.

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EFFECT OF INTRAVENOUS ADMINISTRATION OF HUMAN GROWTH HORMONE ON SULPHATION FACTOR ACTIVITY IN SERUM OF HYPOPITUITARY SUBJECTS

Acta Endocrinologica ◽

10.1530/acta.0.0660491 ◽

1971 ◽

Vol 66 (3) ◽

pp. 491-497 ◽

Cited By ~ 27

Author(s):

Kerstin Hall

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Intravenous Administration ◽

Human Growth ◽

Significant Rise ◽

Mean Difference ◽

Factor Activity ◽

The Mean

ABSTRACT Human growth hormone (HGH) administered as an iv injection of 2–4 mg to hypopituitary patients induced a rise in the levels of sulphation factor (SF) in serum. The low basal levels of SF were not changed during the first hour after HGH injection. Not until three hours after injection, when HGH values approached basal values, there was a significant rise in SF. The mean difference of SF at one and at three hours after HGH injection was 0.52 ± 0.11.

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STIMULATION OF IMMUNOREACTIVE INSULIN AND HUMAN GROWTH HORMONE RELEASE BY ADMINISTRATION OF ARGININE IN PATIENTS WITH DIABETIC RETINOPATHY

Acta Endocrinologica ◽

10.1530/acta.0.0700719 ◽

1972 ◽

Vol 70 (4) ◽

pp. 719-730 ◽

Cited By ~ 6

Author(s):

W. Waldhäusl

Keyword(s):

Growth Hormone ◽

Diabetic Retinopathy ◽

Human Growth Hormone ◽

Human Growth ◽

Immunoreactive Insulin ◽

Insulinogenic Index ◽

Hormone Release ◽

Arginine Hydrochloride ◽

The Mean ◽

Secretory Capacity

ABSTRACT The effect of arginine hydrochloride (30 g), given twice at 90 min intervals, on the release of immunoreactive insulin and of human growth hormone was studied in healthy subjects (HS), in diabetics without and with various degrees of retinopathy and in acromegalics. The insulinogenic index estimated by the ratio of μU IRI per ml/mg per 100 ml of blood glucose was maximal in HS after the first administration of arginine. It was highest in acromegalics and diminished in all the diabetic subjects. The release of human growth hormone as estimated by the mean sum of increments above the basal levels (x̄ + sem) during period I and II was 80.2±15 ng/ml and 59.0±18 ng/ml in HS (n = 7), 38±8.6 ng/ml and 36±16.3 ng/ml in diabetics without retinopathy (n = 9), 4.2±0.4 ng/ml and 19±7 ng/ml in insulin treated diabetics with retinopathy (n = 9), and 22.7±10.7 ng/ml and 46±10.5 ng/ml in orally treated diabetics with retinopathy (n = 7). Patients suffering from proliferative retinopathy (n = 9) exhibited values of 32.8±10.6 ng/ml during period I and 43.5±10.6 ng/ml during period II. The secretory response of HGH to arginine in acromegalics (n = 6) was not significant. The data reported suggest an impaired secretory capacity for the release of human growth hormone to the administration of arginine in patients with diabetic retinopathy. The observations do not support the hypothesis that an exaggerated release of HGH plays a role in the development of diabetic retinopathy.

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EFFECTS OF HUMAN GROWTH HORMONE ON THE SECRETION OF RAT GROWTH HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0860165 ◽

1980 ◽

Vol 86 (1) ◽

pp. 165-169 ◽

Cited By ~ 18

Author(s):

J. O. WILLOUGHBY ◽

MARGARET MENADUE ◽

P. ZEEGERS ◽

P. H. WISE ◽

J. R. OLIVER

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Human Growth ◽

Male Rats ◽

Mean Frequency ◽

Rat Growth ◽

The Mean

Human growth hormone (hGH) was administered to chronically cannulated male rats and its effect upon the physiological secretory patterns of rat growth hormone (rGH) and prolactin were observed. In comparison with injected control animals, a reduction in the size of spontaneous secretory bursts of rGH was apparent when hormone concentrations were compared 3–6 h after administration of hGH (136·27 ± 27·31 (s.e.m.) v. 76·22 ± 20·98 ng/ml respectively). However, the mean frequency of secretory episodes of rGH was unaltered. It is therefore postulated that endogenous rGH may modulate the amplitude but not the rhythmicity of secretory episodes of rGH.

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OBSERVATIONS ON A HAEMAGGLUTINATION—INHIBITION TECHNIQUE FOR ESTIMATING HUMAN GROWTH HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0260219 ◽

1963 ◽

Vol 26 (2) ◽

pp. 219-231 ◽

Cited By ~ 1

Author(s):

P. J. O'CONNOR ◽

L. G. SKINNER

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Haemagglutination Inhibition ◽

Human Growth ◽

Mean Value ◽

Normal Adult ◽

Adult Human ◽

Human Sera ◽

The Mean ◽

Inhibition Technique

SUMMARY The haemagglutination—inhibition technique has been examined as a method of estimating human growth hormone (HGH) and the need for rigid standardization of the procedures involved is stressed. Examination of antisera to a Raben type preparation by immunodiffusion and haemagglutination—inhibition procedures showed the presence of antibodies to albumin and γ-globulin as well as to HGH. The presence of these contaminating antibodies did not appear to interfere with the endpoints obtained in the haemagglutination—inhibition reactions. Within its limitations the technique has been found suitable for the assay of solutions of purified HGH. The mean level of HGH in six normal adult human sera was estimated as 261 ± 23·6 μg./l. (± s.e.) which is similar to the values obtained by other workers, but the validity of this mean value is questioned.

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Human Growth Hormone Secretion During Major Surgical Stress and the Influence of Pre-Treatment with Dexamethasone

Hormone and Metabolic Research ◽

10.1055/s-0028-1097081 ◽

1972 ◽

Vol 4 (01) ◽

pp. 57-57 ◽

Cited By ~ 1

Author(s):

K. Hanssen ◽

V. Asfeldt

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Surgical Stress ◽

Hormone Secretion ◽

Growth Hormone Secretion ◽

Human Growth ◽

Pre Treatment

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Insulin Tolerance Test: human growth hormone response and insulin resistance in primary unipolar depressed, bipolar depressed and control subjects

Psychological Medicine ◽

10.1017/s0033291700043270 ◽

1982 ◽

Vol 12 (1) ◽

pp. 45-55 ◽

Cited By ~ 42

Author(s):

Stephen H. Koslow ◽

Peter E. Stokes ◽

Joseph Mendels ◽

Alan Ramsey ◽

Regina Casper

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Human Growth ◽

Tolerance Test ◽

Insulin Tolerance Test ◽

Control Subjects ◽

Insulin Tolerance ◽

Significant Difference ◽

Pre Treatment ◽

And Control

SynopsisPreliminary data from the National Institute of Mental Health – Clinical Research Branch Collaborative Program on the Psychobiology of Depression dealing with the human growth hormone (hGH) response to the Insulin Tolerance Test (ITT) during the pre-treatment (drug-free) period of the study are presented in this paper. Data are reported for 54 unipolar depressed, 21 bipolar depressed, and 40 normal control subjects, who represent approximately 50% of the final subject sample to be studied. In this population the unipolar depressed subjects showed a significantly greater resistance to insulin-induced hypoglycaemia than bipolar and control subjects. After applying the inclusion/exclusion criteria necessary to interpret hGH responses accurately, the data from only 54 subjects were acceptable. Mean peak hGH concentrations were not significantly different among the three groups. There was, however, a significant difference in the distributions of the hGH peak response, with the bipolar depressed population demonstrating greater variability in response than unipolar and control populations. These findings are discussed as they relate to previous reports and theoretical considerations.

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