Insulin Tolerance Test: human growth hormone response and insulin resistance in primary unipolar depressed, bipolar depressed and control subjects

1982 ◽  
Vol 12 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Stephen H. Koslow ◽  
Peter E. Stokes ◽  
Joseph Mendels ◽  
Alan Ramsey ◽  
Regina Casper
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Control Subjects ◽  
Insulin Tolerance ◽  
Significant Difference ◽  
Pre Treatment ◽  
And Control

SynopsisPreliminary data from the National Institute of Mental Health – Clinical Research Branch Collaborative Program on the Psychobiology of Depression dealing with the human growth hormone (hGH) response to the Insulin Tolerance Test (ITT) during the pre-treatment (drug-free) period of the study are presented in this paper. Data are reported for 54 unipolar depressed, 21 bipolar depressed, and 40 normal control subjects, who represent approximately 50% of the final subject sample to be studied. In this population the unipolar depressed subjects showed a significantly greater resistance to insulin-induced hypoglycaemia than bipolar and control subjects. After applying the inclusion/exclusion criteria necessary to interpret hGH responses accurately, the data from only 54 subjects were acceptable. Mean peak hGH concentrations were not significantly different among the three groups. There was, however, a significant difference in the distributions of the hGH peak response, with the bipolar depressed population demonstrating greater variability in response than unipolar and control populations. These findings are discussed as they relate to previous reports and theoretical considerations.

THE METABOLIC EFFECT OF A SMALL UNIFORM DOSE OF HUMAN GROWTH HORMONE IN HYPOPITUITARY DWARFS AND IN CONTROL CHILDREN

1968 ◽  
Vol 57 (1) ◽  
pp. 129-135 ◽  
Author(s):  
A. Prader ◽  
M. Zachmann ◽  
J. R. Poley ◽  
R. Illig
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Fasting Blood Glucose ◽  
Human Growth ◽  
Diagnostic Value ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Glucose Levels ◽  
Insulin Tolerance ◽  
Physiological Dose

ABSTRACT The effect of small doses of human growth hormone (2 mg/m2 of HGH Raben daily) on the insulin-tolerance test in 15 hypopituitary and 10 control patients is described. With the dose used, fasting blood glucose levels and the response to insulin-induced hypoglycaemia are almost normalized in the hypopituitary patients and have only a slight effect on the control patients. The physiological dose of HGH and the diagnostic value of the insulin-tolerance test in hypopituitarism are discussed.

Shortened Radioimmunoassay for Human Growth Hormone

1974 ◽  
Vol 20 (3) ◽  
pp. 389-391 ◽  
Author(s):  
S L Twomey ◽  
J M Beattie ◽  
G T Wu
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Rank Correlation ◽  
Human Growth ◽  
Statistical Test ◽  
Sign Test ◽  
Assay Mixture ◽  
Signed Rank ◽  
Significant Difference ◽  
Two Temperatures

Abstract We report a radioimmunoassay procedure for human growth hormone in serum, in which the assay mixture is incubated at 37 °C for a total of 5 h rather than at 4 °C for 48 h. There was no significant difference in results by the two methods, according to the Sign Test and the signed-rank statistical test of Wilcoxin. A comparison of results at the two temperatures demonstrated a Spearmann coefficient of rank correlation value of 0.94. With this facilitation, a laboratory can provide results on the same day that the sample arrives.

scholarly journals Clinical Evaluation of Growth Hormone Secretion in Cattle Using Insulin Tolerance Test

2003 ◽  
Vol 65 (7) ◽  
pp. 809-812
Author(s):  
Masafumi OGURO ◽  
Hirotaka ISHIKAWA ◽  
Hiromichi OHTSUKA ◽  
Fumio HOSHI ◽  
Seiichi KAWAMURA
Keyword(s):  
Growth Hormone ◽  
Clinical Evaluation ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Insulin Tolerance

Human growth hormone blunts Na2EDTA-induced hypocalcaemia in hyposomatotrophic children

1983 ◽  
Vol 102 (1) ◽  
pp. 11-15
Author(s):  
Allen W. Root ◽  
Gregory E. Duckett ◽  
Margaret Sweetland ◽  
Catherine Livingston ◽  
Edward O. Reiter
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Ionized Calcium ◽  
Total Calcium ◽  
The Mean ◽  
Pre Treatment ◽  
Basal Concentration ◽  
Immunoreactive Parathyroid Hormone ◽  
Ethylenediamine Tetraacetate

Abstract. An infusion of disodium ethylenediamine tetraacetate (Na2EDTA) (0.13 mmol/kg for 2 h) was administered to 10 hyposomatotrophic children prior to and after 6 and 12 months of treatment with human growth hormone (hGH). Total and ionized calcium and immunoreactive parathyroid hormone (iPTH) concentrations were determined. Mean basal total and ionized calcium concentrations did not change during the year of treatment with hGH. The nadir concentrations of total and ionized calcium increased progressively during hGH administration and after 12 months were significantly increased over pre-treatment values (total calcium: pre-treatment 1.85 ± 0.32 (sd) mmol/l, + 12 months 2.10 ± 0.15, P < 0.01; ionized calcium: pre-treatment 0.55 ± 0.31 mmol/l, + 12 months 0.78 ± 0.14, P < 0.05). The mean basal concentration of iPTH increased slightly after 12 months of hGH administration (pre-treatment 72 ± 18 pg/ml, + 12 months 106 ± 71, P < 0.05), but Na2EDTA-evoked secretion of iPTH was not significant altered by hGH.

Test of growth hormone secretion in adults: poor reproducibility of the insulin tolerance test

1995 ◽  
Vol 133 (3) ◽  
pp. 305-312 ◽  
Author(s):  
Hans C Hoeck ◽  
Peter Vestergaard ◽  
Poul E Jakobsen ◽  
Peter Laurberg
Keyword(s):  
Growth Hormone ◽  
Gh Deficiency ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Repeated Testing ◽  
Provocative Test ◽  
Insulin Tolerance ◽  
Gh Deficiency In Adults

Hoeck HC, Vestergaard P, Jakobsen PE, Laurberg P, Test of growth hormone secretion in adults: poor reproducibility of the insulin tolerance test. Eur J Endocrinol 1995;133:305–12. ISSN 0804–4643 The insulin tolerance test (ITT) is regarded as the most reliable provocative test in the diagnosis of growth hormone (GH) deficiency in adults. In the present study the test was evaluated by investigating the range of GH responses in normal adult males and females and the intra-individual reproducibility of the test. Sixteen healthy non-obese adults, eight males (median age 31.5 years) and eight females (median age 31.8 years) were tested twice with the ITT, with a minimum of 72 h between each test. The females were tested between day 3 and day 10 of their menstrual cycles. Adequate hypoglycemia was achieved in all cases with a median nadir blood glucose of 1.3 mmol/l (range 0.8–2.0). Growth hormone in serum was measured by immunoradiometric assay and low values were confirmed by a different assay. Median peak GH concentration responses to the ITT were: in males 27.9 μg/l, range 5.0–71.1 (test 1) and 30.5 μg/l, range 7.9–69.5 (test 2); and in females 9.0 μg/l, range 4.1–17.9 (test 1) and 8.4 μg/l, range 0.09–42.4 (test 2). The rise in GH concentration during the ITT was higher in males than in females. In the males, all stimulated GH values were ≥5.0 μg/l. In the females, four out of 16 tests gave values below 5.0 μg/l and in one test the GH value was around the detection limit of the assays. There was poor reproducibility during repeated testing, with no correlation between the results of the two tests. The results did not correlate to the magnitude of the hypoglycemia. The results of this study illustrate the complexity of the regulation of GH secretion and indicate that the ITT is less useful for diagnosing GH deficiency in adults than previously anticipated. The diagnosis of GH deficiency in adults and especially in adult females should not be based on the results of a single ITT alone. Hans C Hoeck, Department of Endocrinology, Aalborg Regional Hospital, Reberbansgade, DK-9000 Aalborg, Denmark

scholarly journals In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized inB. moriPupae

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Hanglian Lan ◽  
Zuoming Nie ◽  
Yue Liu ◽  
Zhengbing Lv ◽  
Yingshuo Liu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Recombinant Human Growth Hormone ◽  
Expression System ◽  
Human Growth ◽  
The Body ◽  
Control Group ◽  
Prokaryotic Expression System ◽  
Significant Difference ◽  
Baculovirus System

The human growth hormone (hGH) has been expressed in prokaryotic expression system with low bioactivity previously. Then the effectiveB. moribaculovirus system was employed to express hGH identical to mature hGH successfully in larvae, but the expression level was still limited. In this work, the hGH was expressed inB. moripupae by baculovirus system. Quantification of recombinant hGH protein (BmrhGH) showed that the expression of BmrhGH reached the level of approximately 890 μg/mL pupae supernatant solution, which was five times more than the level using larvae. Furthermore, Animals were gavaged with BmrhGH at the dose of 4.5 mg/rat.day, and the body weight gain (BWG) of treated group had a significant difference (P<.01) compared with the control group. The other two parameters of liver weight and epiphyseal width were also found to be different between the two groups (P<.05). The results suggested that BmrhGH might be used as a protein drug by oral administration.

Comparison of pituitary–adrenal responsiveness between insulin tolerance test and growth hormone-releasing peptide-2 test: A pilot study

Peptides ◽  
2010 ◽  
Vol 31 (4) ◽  
pp. 657-661 ◽  
Author(s):  
Toshiko Kano ◽  
Hitoshi Sugihara ◽  
Mariko Sudo ◽  
Mototsugu Nagao ◽  
Taro Harada ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Pilot Study ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Insulin Tolerance
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