Sox9-dependent expression of Gstm6 in Sertoli cells during testis development in mice

Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes that play a role in the protection of tissues by the detoxification of hazardous and carcinogenic compounds. We found previously that Gstm6 is upregulated in the somatic cells of male mouse fetal gonads relative to female gonads. In this study, we describe the spatial and temporal expression pattern of Gstm6 during mouse development. We show that Gstm6 is predominantly expressed in the reproductive system, at significantly higher levels in XY gonads compared with XX gonads from 11.5 dpc onwards, and remains expressed in the testes in adult mice. Its expression is associated with the Sertoli cell lineage, and is dependent on the expression of the male sex-determining gene Sox9. Our data suggest that Gstm6 plays a male-specific role in gonad development or function, possibly by modulating the exposure of somatic tissue and/or germ cells to endogenous or exogenous toxicants.

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Loss of the histone chaperone ASF1B reduces female reproductive capacity in mice

Reproduction ◽

10.1530/rep-15-0327 ◽

2016 ◽

Vol 151 (5) ◽

pp. 477-489 ◽

Cited By ~ 9

Author(s):

S Messiaen ◽

J Guiard ◽

C Aigueperse ◽

I Fliniaux ◽

S Tourpin ◽

...

Keyword(s):

Gonad Development ◽

Cell Lineage ◽

Reproductive Capacity ◽

Chromatin Modifications ◽

Mouse Development ◽

Differentiation Potential ◽

Female Mice ◽

Evolutionarily Conserved ◽

Meiotic Entry

Abstract Anti-silencing function 1 (ASF1) is an evolutionarily conserved histone H3–H4 chaperone involved in the assembly/disassembly of nucleosome and histone modification. Two paralogous genes, Asf1a and Asf1b, exist in the mouse genome. Asf1a is ubiquitously expressed and its loss causes embryonic lethality. Conversely, Asf1b expression is more restricted and has been less studied. To determine the in vivo function of Asf1b, we generated a Asf1b-deficient mouse line (Asf1bGT(ROSA-βgeo)437) in which expression of the lacZ reporter gene is driven by the Asf1b promoter. Analysis of β-galactosidase activity at early embryonic stages indicated a correlation between Asf1b expression and cell differentiation potential. In the gonads of both male and female, Asf1b expression was specifically detected in the germ cell lineage with a peak expression correlated with meiosis. The viability of Asf1b-null mice suggests that Asf1b is dispensable for mouse development. However, these mice showed reduced reproductive capacity compared with wild-type controls. We present evidence that the timing of meiotic entry and the subsequent gonad development are affected more severely in Asf1b-null female mice than in male mice. In female mice, in addition to subfertility related to altered gamete formation, variable defects compromising the development and/or survival of their offspring were also observed. Altogether, our data indicate the importance of Asf1b expression at the time of meiotic entry, suggesting that chromatin modifications may play a central role in this process.

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SPATIAL AND TEMPORAL EXPRESSION PATTERN OF KIS DURING MOUSE DEVELOPMENT

Cardiovascular Pathology ◽

10.1016/j.carpath.2004.03.212 ◽

2004 ◽

Vol 13 (3) ◽

pp. 71

Author(s):

Michelle Olive ◽

Manfred Boehm ◽

Martin Crook ◽

Xuan Qu ◽

Elizabeth Nabel

Keyword(s):

Expression Pattern ◽

Temporal Expression ◽

Mouse Development ◽

Temporal Expression Pattern

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A male-specific role for SOX9 in vertebrate sex determination

Development ◽

10.1242/dev.122.9.2813 ◽

1996 ◽

Vol 122 (9) ◽

pp. 2813-2822 ◽

Cited By ~ 12

Author(s):

J. Kent ◽

S.C. Wheatley ◽

J.E. Andrews ◽

A.H. Sinclair ◽

P. Koopman

Keyword(s):

Sex Determination ◽

In Situ Hybridisation ◽

Gonad Development ◽

Adult Life ◽

Cell Lineage ◽

Polyclonal Antiserum ◽

Cell Type Specificity ◽

Sox9 Expression ◽

Specific Expression ◽

Male Specific

Mutation analyses of patients with campomelic dysplasia, a bone dysmorphology and XY sex reversal syndrome, indicate that the SRY-related gene SOX9 is involved in both skeletal development and sex determination. To clarify the role SOX9 plays in vertebrate sex determination, we have investigated its expression during gonad development in mouse and chicken embryos. In the mouse, high levels of Sox9 mRNA were found in male (XY) but not female (XX) genital ridges, and were localised to the sex cords of the developing testis. Purified fetal germ cells lacked Sox9 expression, indicating that Sox9 expression is specific to the Sertoli cell lineage. Sex specificity of SOX9 protein expression was confirmed using a polyclonal antiserum. The timing and cell-type specificity of Sox9 expression suggests that Sox9 may be directly regulated by SRY. Male-specific expression of cSOX9 mRNA during the sex determination period was also observed in chicken genital ridges. The conservation of sexually dimorphic expression in two vertebrate classes which have significant differences in their sex determination mechanisms, points to a fundamental role for SOX9 in testis determination in vertebrates. Sox9 expression was maintained in the mouse testis during fetal and adult life, but no expression was seen at any stage by in situ hybridisation in the developing ovary. Male-specific expression was also observed in the cells surrounding the Mullerian ducts and in the epididymis, and expression in both sexes was detected in the developing collecting ducts of the metanephric kidney. These results suggest that SOX9 may have a wider role in the development of the genitourinary system.

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Expression of an Androgenic Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation

ISRN Endocrinology ◽

10.5402/2011/476283 ◽

2011 ◽

Vol 2011 ◽

pp. 1-11 ◽

Cited By ~ 35

Author(s):

Tomer Ventura ◽

Rivka Manor ◽

Eliahu D. Aflalo ◽

Simy Weil ◽

Isam Khalaila ◽

...

Keyword(s):

Sexual Differentiation ◽

Reproductive Strategies ◽

Sex Differentiation ◽

Macrobrachium Rosenbergii ◽

Androgenic Gland ◽

Temporal Expression ◽

Androgenic Hormone ◽

Temporal Expression Pattern ◽

Male Specific ◽

Specific Insulin

The crustacean male-specific androgenic gland (AG) regulates sexual differentiation. In the prawn Macrobrachium rosenbergii, silencing an AG-specific insulin-like encoding transcript (Mr-IAG) inhibited the development of male sexual characters, suggesting that Mr-IAG is a key androgenic hormone. We used recombinant pro-Mr-IAG peptide to generate antibodies that recognized the peptide in AG cells and extracts, as verified by mass spectrometry. We revealed the temporal expression pattern of Mr-IAG and studied its relevance to the timetable of sex differentiation processes in juveniles and after puberty. Mr-IAG was expressed from as early as 20 days after metamorphosis, prior to the appearance of external male sexual characters. Mr-IAG expression was lower in the less reproductively active orange-clawed males than in both the dominant blue-clawed males and the actively sneak mating small males. These results suggest a role for Mr-IAG both in the timing of male sexual differentiation and in regulating reproductive strategies.

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Faculty Opinions recommendation of Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725255586.793504217 ◽

2015 ◽

Author(s):

Keith Jones ◽

Guillaume Halet

Keyword(s):

Stem Cells ◽

Cell Lineage ◽

Lineage Tracing ◽

Germline Stem Cells ◽

Adult Mice

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Temporal expression pattern of progesterone receptor in the uterine luminal epithelium suggests its requirement during early events of implantation

Fertility and Sterility ◽

10.1016/j.fertnstert.2011.01.160 ◽

2011 ◽

Vol 95 (6) ◽

pp. 2087-2093 ◽

Cited By ~ 28

Author(s):

Honglu Diao ◽

Bibhash C. Paria ◽

Shuo Xiao ◽

Xiaoqin Ye

Keyword(s):

Progesterone Receptor ◽

Expression Pattern ◽

Luminal Epithelium ◽

Temporal Expression ◽

Temporal Expression Pattern

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The spatial and temporal expression pattern of sevenless is exclusively controlled by gene-internal elements.

The EMBO Journal ◽

10.1002/j.1460-2075.1989.tb08367.x ◽

1989 ◽

Vol 8 (8) ◽

pp. 2381-2386 ◽

Cited By ~ 49

Author(s):

K. Basler ◽

P. Siegrist ◽

E. Hafen

Keyword(s):

Expression Pattern ◽

Temporal Expression ◽

Temporal Expression Pattern

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The SH2-Containing Inositol Polyphosphate 5-Phosphatase, Ship, Is Expressed During Hematopoiesis and Spermatogenesis

Blood ◽

10.1182/blood.v91.8.2753.2753_2753_2759 ◽

1998 ◽

Vol 91 (8) ◽

pp. 2753-2759 ◽

Cited By ~ 66

Author(s):

Qiurong Liu ◽

Fouad Shalaby ◽

Jamie Jones ◽

Denis Bouchard ◽

Daniel J. Dumont

Keyword(s):

Primitive Streak ◽

Inositol Polyphosphate ◽

Mouse Development ◽

Developmentally Regulated ◽

Adult Mice ◽

Expression Studies ◽

Cell Culture Systems ◽

Physiologic Function ◽

T Cell Maturation

Ship is a recently identified SH2-containing inositol polyphosphate 5-phosphatase that has been implicated as an important signaling molecule in cell-culture systems. To understand the physiologic function of Ship in vivo, we performed expression studies of Ship during mouse development. Results of this study demonstrate the expression of ship to be in late primitive-streak stage embryos (7.5 days postcoitus [dpc]), when hematopoiesis is thought to begin, and the expression is restricted to the hematopoietic lineage in mouse embryo. In adult mice, Ship expression continues to be in the majority of cells from hematopoietic origin, including granulocytes, monocytes, and lymphocytes, and is also found in the spermatids of the testis. Furthermore, the level of Ship expression is developmentally regulated during T-cell maturation. These results suggest a possible role for Ship in the differentiation and maintenance of the hematopoietic lineages and in spermatogenesis.

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Normal Immune System Development in Mice Lacking the Deltex-1 RING Finger Domain

Molecular and Cellular Biology ◽

10.1128/mcb.25.4.1437-1445.2005 ◽

2005 ◽

Vol 25 (4) ◽

pp. 1437-1445 ◽

Cited By ~ 15

Author(s):

Sébastien Storck ◽

Frédéric Delbos ◽

Nicolas Stadler ◽

Catherine Thirion-Delalande ◽

Florence Bernex ◽

...

Keyword(s):

B Cell ◽

Notch Signaling ◽

Ubiquitin Ligase ◽

Cell Lineage ◽

Ring Finger ◽

Mouse Development ◽

Ring Finger Domain ◽

Ubiquitin Ligase Activity ◽

Immune System Development ◽

Cell Commitment

ABSTRACT The Notch signaling pathway controls several cell fate decisions during lymphocyte development, from T-cell lineage commitment to the peripheral differentiation of B and T lymphocytes. Deltex-1 is a RING finger ubiquitin ligase which is conserved from Drosophila to humans and has been proposed to be a regulator of Notch signaling. Its pattern of lymphoid expression as well as gain-of-function experiments suggest that Deltex-1 regulates both B-cell lineage and splenic marginal-zone B-cell commitment. Deltex-1 was also found to be highly expressed in germinal-center B cells. To investigate the physiological function of Deltex-1, we generated a mouse strain lacking the Deltex-1 RING finger domain, which is essential for its ubiquitin ligase activity. Deltex-1Δ/Δ mice were viable and fertile. A detailed histological analysis did not reveal any defects in major organs. T- and B-cell development was normal, as were humoral responses against T-dependent and T-independent antigens. These data indicate that the Deltex-1 ubiquitin ligase activity is dispensable for mouse development and immune function. Possible compensatory mechanisms, in particular those from a fourth Deltex gene identified during the course of this study, are also discussed.

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