scholarly journals Expression of an Androgenic Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Tomer Ventura ◽  
Rivka Manor ◽  
Eliahu D. Aflalo ◽  
Simy Weil ◽  
Isam Khalaila ◽  
...  
Keyword(s):  
Sexual Differentiation ◽  
Reproductive Strategies ◽  
Sex Differentiation ◽  
Macrobrachium Rosenbergii ◽  
Androgenic Gland ◽  
Temporal Expression ◽  
Androgenic Hormone ◽  
Temporal Expression Pattern ◽  
Male Specific ◽  
Specific Insulin

The crustacean male-specific androgenic gland (AG) regulates sexual differentiation. In the prawn Macrobrachium rosenbergii, silencing an AG-specific insulin-like encoding transcript (Mr-IAG) inhibited the development of male sexual characters, suggesting that Mr-IAG is a key androgenic hormone. We used recombinant pro-Mr-IAG peptide to generate antibodies that recognized the peptide in AG cells and extracts, as verified by mass spectrometry. We revealed the temporal expression pattern of Mr-IAG and studied its relevance to the timetable of sex differentiation processes in juveniles and after puberty. Mr-IAG was expressed from as early as 20 days after metamorphosis, prior to the appearance of external male sexual characters. Mr-IAG expression was lower in the less reproductively active orange-clawed males than in both the dominant blue-clawed males and the actively sneak mating small males. These results suggest a role for Mr-IAG both in the timing of male sexual differentiation and in regulating reproductive strategies.

scholarly journals Sox9-dependent expression of Gstm6 in Sertoli cells during testis development in mice

Reproduction ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 481-486 ◽  
Author(s):  
Annemiek Beverdam ◽  
Terje Svingen ◽  
Stefan Bagheri-Fam ◽  
Pascal Bernard ◽  
Peter McClive ◽  
...  
Keyword(s):  
Gonad Development ◽  
Cell Lineage ◽  
Somatic Tissue ◽  
Temporal Expression ◽  
Mouse Development ◽  
Adult Mice ◽  
Multifunctional Enzymes ◽  
Male Sex ◽  
Temporal Expression Pattern ◽  
Male Specific

Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes that play a role in the protection of tissues by the detoxification of hazardous and carcinogenic compounds. We found previously that Gstm6 is upregulated in the somatic cells of male mouse fetal gonads relative to female gonads. In this study, we describe the spatial and temporal expression pattern of Gstm6 during mouse development. We show that Gstm6 is predominantly expressed in the reproductive system, at significantly higher levels in XY gonads compared with XX gonads from 11.5 dpc onwards, and remains expressed in the testes in adult mice. Its expression is associated with the Sertoli cell lineage, and is dependent on the expression of the male sex-determining gene Sox9. Our data suggest that Gstm6 plays a male-specific role in gonad development or function, possibly by modulating the exposure of somatic tissue and/or germ cells to endogenous or exogenous toxicants.

scholarly journals Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Linn S. Mamsen ◽  
Emil H. Ernst ◽  
Rehannah Borup ◽  
Agnete Larsen ◽  
Rasmus H. Olesen ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Sex Differentiation ◽  
Temporal Expression ◽  
Temporal Expression Pattern

scholarly journals Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Endocrinology ◽  
2008 ◽  
Vol 150 (3) ◽  
pp. 1278-1286 ◽  
Author(s):  
Tomer Ventura ◽  
Rivka Manor ◽  
Eliahu D. Aflalo ◽  
Simy Weil ◽  
Shaul Raviv ◽  
...  
Keyword(s):  
Gender Differences ◽  
Growth Parameters ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Sex Characteristics ◽  
Specific Gene ◽  
Androgenic Gland ◽  
Sperm Duct ◽  
Specific Insulin

Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of an AG cDNA subtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergii insulin-like AG (Mr-IAG) gene. The cDNA of this gene was then cloned and fully sequenced. The cysteine backbone of the predicted mature Mr-IAG peptide (B and A chains) showed high similarity to that of other crustacean AG-specific insulin-like peptides. In vivo silencing of the gene, by injecting the prawns with Mr-IAG double-stranded RNA, temporarily prevented the regeneration of male secondary sexual characteristics, accompanied by a lag in molt and a reduction in growth parameters, which are typically higher in males of the species. In terms of reproductive parameters, silencing of Mr-IAG led to the arrest of testicular spermatogenesis and of spermatophore development in the terminal ampullae of the sperm duct, accompanied by hypertrophy and hyperplasia of the AGs. This study constitutes the first report of the silencing of a gene expressed specifically in the AG, which caused a transient adverse effect on male phenotypical gender differences and spermatogenesis. Temporal silencing of a newly identified insulin-like gene from prawn androgenic gland inhibits primary spermatogenesis, male secondary sex characteristics, and growth.

scholarly journals Lentiviral-Transduced Ectopic Expression of Androgenic Hormone in a Crustacean Hematopoietic Primary Cell Culture

2021 ◽  
Vol 8 ◽  
Author(s):  
Noa Rotem-Dai ◽  
Simy Weil ◽  
Yariv Greenshpan ◽  
Shai Abehsera ◽  
Rivka Manor ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Lentiviral Vector ◽  
Ectopic Expression ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Androgenic Gland ◽  
Giant Freshwater Prawn ◽  
Androgenic Hormone ◽  
Basic And Applied Research ◽  
Early Developmental

Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture, since the females can be stocked at higher densities and exhibit more uniform growth than males. Recently, a novel biotechnology for all-female aquaculture was developed based on injection into post-larvae of suspended primary AG cells from mature M. rosenbergii males. However, this biotechnology depends on the availability of appropriate male donors and it requires delicate surgical procedures on the small endocrine AG to produce appropriate quantities of cells for the sex manipulation. We therefore established a new platform for the production of M. rosenbergii IAG (Mr-IAG) in hematopoietic cells. The method rests on the induction of ectopic Mr-IAG expression under the white spot syndrome virus (WSSV) immediate-early 1 (IE1) promoter via a novel lentiviral vector. Our results demonstrate that M. rosenbergii primary cells infected with the Mr-IAG lentiviruses are capable of transcription, translation, and secretion of Mr-IAG in culture. Our new platform, which produces easy-to-harvest cells in abundance, could replace the AG cells used in the first step of the above-mentioned biotechnology for all-female aquaculture and, importantly, pave the way for producing monosex populations in other edible crustacean aquaculture species. In addition, a lentiviral system for crustacean cells provides a useful tool for basic and applied research in crustacean species.

scholarly journals The spatial and temporal expression pattern of Nesp and its antisense Nespas, in mid-gestation mouse embryos

2001 ◽  
Vol 100 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Simon T. Ball ◽  
Christine M. Williamson ◽  
Christopher Hayes ◽  
Terry Hacker ◽  
Jo Peters
Keyword(s):  
Expression Pattern ◽  
Mouse Embryos ◽  
Temporal Expression ◽  
Temporal Expression Pattern

scholarly journals Sex Differentiation in Amphibians: Effect of Temperature and Its Influence on Sex Reversal

2021 ◽  
pp. 1-11
Author(s):  
Adrián Ruiz-García ◽  
Álvaro S. Roco ◽  
Mónica Bullejos
Keyword(s):  
Sexual Differentiation ◽  
Sex Differentiation ◽  
Environmental Contaminants ◽  
Natural Populations ◽  
Sex Reversal ◽  
The Other ◽  
Effect Of Temperature ◽  
Amphibian Species ◽  
Expected Increase

The role of environmental factors in sexual differentiation in amphibians is not new. The effect of hormones or hormone-like compounds is widely demonstrated. However, the effect of temperature has traditionally been regarded as something anecdotal that occurs in extreme situations and not as a factor to be considered. The data currently available reveal a different situation. Sexual differentiation in some amphibian species can be altered even by small changes in temperature. On the other hand, although not proven, it is possible that temperature is related to the appearance of sex-reversed individuals in natural populations under conditions unrelated to environmental contaminants. According to this, temperature, through sex reversal (phenotypic sex opposed to genetic sex), could play an important role in the turnover of sex-determining genes and in the maintenance of homomorphic sex chromosomes in this group. Accordingly, and given the expected increase in global temperatures, growth and sexual differentiation in amphibians could easily be affected, altering the sex ratio in natural populations and posing major conservation challenges for a group in worldwide decline. It is therefore particularly urgent to understand the mechanism by which temperature affects sexual differentiation in amphibians.

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