Exogenous androgens reduce the expression of INSL3, a hormone involved in normal testicular descent, in fetal Leydig cells

2014 ◽  
Author(s):  
W Colin Duncan ◽  
Fiona Connolly ◽  
Lyndsey Boswell ◽  
Graeme Burt ◽  
Alan S McNeilly ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Testicular Descent ◽  
Fetal Leydig Cells

Distinctive functioning of STARD1 in the fetal Leydig cells compared to adult Leydig and adrenal cells. Impact of Hedgehog signaling via the primary cilium.

2021 ◽  
pp. 111265
Author(s):  
Anbarasi Kothandapani ◽  
Michele Campaigne Larsen ◽  
Jinwoo Lee ◽  
Joan S. Jorgensen ◽  
Colin R. Jefcoate
Keyword(s):  
Primary Cilium ◽  
Leydig Cells ◽  
Hedgehog Signaling ◽  
Adrenal Cells ◽  
Fetal Leydig Cells

CYTOPLASMIC FILAMENTS IN FETAL LEYDIG CELLS

1982 ◽  
Vol 383 (1 The Cell Biol) ◽  
pp. 486-487 ◽  
Author(s):  
Lauri J. Pelliemi ◽  
Martin Dym ◽  
Jorma Paranko
Keyword(s):  
Leydig Cells ◽  
Cytoplasmic Filaments ◽  
Fetal Leydig Cells

scholarly journals Mamld1 Deficiency Significantly Reduces mRNA Expression Levels of Multiple Genes Expressed in Mouse Fetal Leydig Cells but Permits Normal Genital and Reproductive Development

Endocrinology ◽  
2012 ◽  
Vol 153 (12) ◽  
pp. 6033-6040 ◽  
Author(s):  
Mami Miyado ◽  
Michiko Nakamura ◽  
Kenji Miyado ◽  
Ken-ichirou Morohashi ◽  
Shinichiro Sano ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Leydig Cells ◽  
Reproductive Development ◽  
Expression Levels ◽  
Multiple Genes ◽  
Mrna Expression Levels ◽  
Fetal Leydig Cells

Immunolocalisation of androgen receptor to interstitial cells in fetal rat testes and to mesenchymal and epithelial cells of associated ducts

1995 ◽  
Vol 147 (2) ◽  
pp. 285-293 ◽  
Author(s):  
G Majdic ◽  
M R Millar ◽  
P T K Saunders
Keyword(s):  
Androgen Receptor ◽  
Epithelial Cells ◽  
Leydig Cells ◽  
Interstitial Cells ◽  
Mesenchymal Cells ◽  
Wolffian Duct ◽  
Nuclear Staining ◽  
Mullerian Duct ◽  
Fetal Rat ◽  
Fetal Leydig Cells

Abstract Androgens are required for the development of male internal and external genitalia. Androgen action is mediated by an intracellular receptor which acts as a transcription factor following activation by ligand binding. The aim of the present study was to define the time of appearance of androgen receptor (AR) in the male fetal rat gonad using immunohistochemistry. Intact fetuses (days 13·5–16·5) or testicular tissue (days 16·5–20·5 and days 3–7 postnatal) were fixed in Bouins' solution and processed into paraffin wax. On day 16·5 nuclear AR were present in mesenchymal cells surrounding the Wolffian duct but those around the Mullerian duct were receptor negative. During the following day (17–18) the abundance of nuclear staining increased, becoming detectable in the epithelial cells of the Wolffian ducts. Within the testis some nuclear staining was apparent at day 17 but was confined to interstitial cells surrounding the seminiferous cords. As development of the testis proceeded the abundance of nuclear AR in peritubular and elongated mesenchymal cells increased. AR were not detected in fetal Leydig cells expressing 3β-hydroxysteroid dehydrogenase nor in the ovaries or associated ducts of female fetuses at the same ages. In conclusion, in the rat we have found AR expression detectable by immunohistochemistry in mesonephric mesenchyme to be confined to that underlying the Wolffian ducts and to be absent from the area around the degenerating Mullerian duct. On and after day 17 of gestation AR is present in Wolffian duct epithelial cell nuclei and within the testis it is confined to peritubular and interstitial cells which may have migrated from the mesonephros. Fetal Leydig cells were receptor negative. Within the seminiferous cords AR in Sertoli cells remained low until after birth and some perinuclear staining was detected in cells thought to be gonocytes. We believe this to be the first report of immunolocalisation of AR to fetal testicular interstitial cells. Journal of Endocrinology (1995) 147, 285–293

An insight into testis and gubernaculum dynamics of INSL3 - RXFP2 signalling during testicular descent in the dog

2010 ◽  
Vol 22 (5) ◽  
pp. 751 ◽  
Author(s):  
S. Arrighi ◽  
G. Bosi ◽  
D. Groppetti ◽  
M. Aralla ◽  
F. Cremonesi
Keyword(s):  
Sertoli Cells ◽  
Leydig Cells ◽  
Feedback Loop ◽  
Testicular Descent ◽  
Fetal Period ◽  
Male Development ◽  
Dog Testis ◽  
Gubernaculum Testis ◽  
Insight Into ◽  
Different Tissues

Insulin-like 3 (INSL3) plays a prominent role in male development and is supposed to induce the growth of the gubernaculum testis (g.t.), thus being directly involved in testicular descent in humans and rodents. This happens through activation of the RXFP2 receptor (GREAT or LGR8). The INSL3–RXFP2 complex is reputed to play an additional paracrine role in the testis, possibly acting as part of an autocrine feedback loop. The present work provides evidence of the immunolocalisation of INSL3 in the Leydig cells of canine fetuses and of the expression of RXFP2 receptor in different tissues of the g.t. of the same specimens. RXFP2 was localised at the cell membrane of g.t. muscle and connective cells, as well as in the epithelial cells of the developing excurrent ducts. Notably, RXFP2 immunoreactivity of the g.t. was limited to fetuses at ~35–45 days of gestation, which is also the fetal period when the endocrine compartment of the dog testis is active endocrinologically, as confirmed by the anti-P450c17 and anti-INSL3 immunoreactivities of the fetal Leydig cells, and by anti-Müllerian hormone immunoreactivity of the Sertoli cells. The same immunoreactivities were also evaluated in the testes of cryptorchid dogs of different ages. RXFP2 immunoreactivity was absent from genital tracts of cryptorchid testes and g.t. remnants.

Luteinizing hormone-dependent activity and luteinizing hormone-independent differentiation of rat fetal Leydig cells

2001 ◽  
Vol 172 (1-2) ◽  
pp. 193-202 ◽  
Author(s):  
Stéphanie Migrenne ◽  
Catherine Pairault ◽  
Chrystèle Racine ◽  
Gabriel Livera ◽  
Annette Géloso ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Leydig Cells ◽  
Dependent Activity ◽  
Fetal Leydig Cells

scholarly journals A Transcriptome-Wide Screen for mRNAs Enriched in Fetal Leydig Cells: CRHR1 Agonism Stimulates Rat and Mouse Fetal Testis Steroidogenesis

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47359 ◽  
Author(s):  
Erin N. McDowell ◽  
Anne E. Kisielewski ◽  
Jack W. Pike ◽  
Heather L. Franco ◽  
Humphrey H-C. Yao ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Fetal Testis ◽  
Fetal Leydig Cells

scholarly journals Cellular Microenvironment Dictates Androgen Production by Murine Fetal Leydig Cells in Primary Culture1

2014 ◽  
Vol 91 (4) ◽  
Author(s):  
Colleen M. Carney ◽  
Jessica L. Muszynski ◽  
Lindsay N. Strotman ◽  
Samantha R. Lewis ◽  
Rachel L. O'Connell ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Cellular Microenvironment ◽  
Fetal Leydig Cells

280. INSL3 is a measure of human Leydig cell functionality both during fetal and adult life

2008 ◽  
Vol 20 (9) ◽  
pp. 80
Author(s):  
R. Anand-Ivell ◽  
J. Manson ◽  
G. Wittert ◽  
J. Wohlgemuth ◽  
B. Hafen ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Cell Function ◽  
Adult Life ◽  
Physiological Role ◽  
South Australia ◽  
Testicular Descent ◽  
Adult Type ◽  
Hpg Axis ◽  
Age Related

Insulin like factor 3 (INSL3) and testosterone are the two major secretory products of the testis, both produced by the interstitial Leydig cells. The Leydig cells of the testis have two distinct generations, one developing before birth (fetal Leydig cells, FLC) and an adult type (adult Leydig cells, ALC) that become differentiated and functional at puberty. Although these two types of Leydig cells represent distinct populations, rodent studies show that both types produce testosterone and INSL3. Both are presumed to have evolved from a common stem cell pool. We measured INSL3 levels in human amniotic fluids collected at various times of gestation and show for the first time that the human male fetus indeed generates INSL3 at a time appropriate for the first transabdominal phase of testicular descent, which appears to be the primary physiological role for the fetal hormone. INSL3 appears to be independent of androgen production. The adult type Leydig cells (in adult men) secrete INSL3 that can be measured in the peripheral circulation at levels ranging from 0.5 to 2.5 ng/mL. We studied a large randomly recruited cohort of 1183 men from South Australia, comparing serum INSL3 concentrations with age, and a variety of endocrine, cognitive and morphological parameters. INSL3 concentration was observed to decline significantly with age. This however, had no correlation with testosterone or components of the HPG axis. INSL3 is an independent measure of Leydig cell function (quality and number), which appears to be independent of acute control via the HPG axis. Its decline with age reflects a decline in the properties of the Leydig cell population only, and emphasises a gonadal component in the age-related decrease in androgen production. Research supported by ARC Discovery grant DP0773315.

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