scholarly journals Nosemosis en colmenas de abejas africanizadas (Apis mellifera) en las condiciones tropicales de Costa Rica: Nosema apis o Nosema ceranae

2021 ◽  
Vol 39 (2) ◽  
pp. 1-8
Author(s):  
Rafael A. Calderón ◽  
Luis A. Sánchez
Keyword(s):  
Apis Mellifera ◽  
Costa Rica ◽  
Nosema Ceranae ◽  
Nosema Apis

Se estudió la presencia del microsporidio Nosema spp. en colmenas de abejas africanizadas en Costa Rica. Se seleccionaron un total de 75 muestras de abejas adultas de diferentes zonas apícolas del país, para el diagnóstico molecular de nosemosis. Previamente a la prueba de PCR, las esporas de Nosema spp. se identificaron morfológicamente con el microscopio de luz a un aumento de 40x. Con base en el análisis molecular, se determinó que la mayoría de abejas estaban infectadas con Nosema ceranae, aun cuando las colmenas no mostraban signos clínicos de la infección durante el periodo de muestreo. Por otra parte, ninguna de las muestras estaba infectada con Nosema apis. Un hallazgo para resaltar es que un 29.3% de las muestras de abejas resultaron negativas a nosemosis mediante el examen de PCR. El origen de las abejas, las cuales se colectaron de apiarios ubicados en cuatro de las siete provincias de Costa Rica, indica que el microsporidio N. ceranae está ampliamente distribuido en las principales zonas apícolas del país. Aún no se conoce con exactitud las consecuencias patológicas de la presencia de N. ceranae en colmenas de abejas africanizadas. Sin embargo, debido a la pérdida de abejas melíferas reportada en Europa, relacionada a infecciones de microsporidios, la virulencia de N. ceranae en abejas africanizadas debe ser estudiada.

scholarly journals Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies

2019 ◽  
Vol 63 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Asli Özkırım ◽  
Aygün Schiesser ◽  
Nevіn Keskin
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Winter Season ◽  
Nosema Ceranae ◽  
Molecular Techniques ◽  
Winter Period ◽  
Nosema Apis ◽  
Infection Level ◽  
Seasonal Infection ◽  
Asian Honeybee

AbstractNosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.

scholarly journals Population Genetics of Nosema apis and Nosema ceranae: One Host (Apis mellifera) and Two Different Histories

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145609 ◽  
Author(s):  
Xulio Maside ◽  
Tamara Gómez-Moracho ◽  
Laura Jara ◽  
Raquel Martín-Hernández ◽  
Pilar De la Rúa ◽  
...  
Keyword(s):  
Population Genetics ◽  
Apis Mellifera ◽  
Nosema Ceranae ◽  
Nosema Apis

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4252 ◽  
Author(s):  
Rositsa Shumkova ◽  
Ani Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Ssu Rdna ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

Comparison of the energetic stress associated with experimental Nosema ceranae and Nosema apis infection of honeybees (Apis mellifera)

2011 ◽  
Vol 109 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Raquel Martín-Hernández ◽  
Cristina Botías ◽  
Laura Barrios ◽  
Amparo Martínez-Salvador ◽  
Aránzazu Meana ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Energetic Stress

Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis?

2011 ◽  
Vol 14 (8) ◽  
pp. 2127-2138 ◽  
Author(s):  
Raquel Martín-Hernández ◽  
Cristina Botías ◽  
Encarna Garrido Bailón ◽  
Amparo Martínez-Salvador ◽  
Lourdes Prieto ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Nosema Ceranae ◽  
Nosema Apis

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

2017 ◽  
Author(s):  
Peter Hristov ◽  
Any Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts ◽  
Rate Of Invasion

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in honey bee Apis mellifera. The object of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the Bulgarian honey bee. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest rate of invasion (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but like in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection in Bulgarian honey bee that confirms the worldwide dissemination and prevalence of Nosema ceranae.

scholarly journals Evaluation of Antimicrosporidian Activity of Plant Extracts on Nosema ceranae

2016 ◽  
Vol 60 (2) ◽  
pp. 167-178 ◽  
Author(s):  
Jeong Hwa Kim ◽  
Jin Kyu Park ◽  
Jae Kwon Lee
Keyword(s):  
Apis Mellifera ◽  
Cell Types ◽  
Mechanisms Of Action ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Microsporidian Species ◽  
Infected Cells ◽  
Multiple Cell ◽  
Artemisia Dubia ◽  
Toxic Concentrations

Abstract Nosemosis is one of the most common protozoan diseases of adult bees (Apis mellifera). Nosemosis is caused by two species of microsporidia; Nosema apis and Nosema ceranae. Nosema ceranae is potentially more dangerous because it has the ability to infect multiple cell types, and it is now the predominant microsporidian species in A. mellifera. In this study, we identified two anti-nosemosis plants, Aster scaber and Artemisia dubia, which reduced the spore development of N. ceranae in spore-infected cells. The most important aspect of our results was that our treatment was effective at non-toxic concentrations. Anti-nosemosis activities of both plants were revealed in honey bee experiments. Specifically, a mixed extract of both A. scaber and A. dubia showed stronger activity than treatment with each single extract alone. Although the mechanisms of action of A. scaber and A. dubia against N. ceranae are still unclear, our results suggest new medicaments and therapeutic methods to control N. ceranae infection.

Comparative virulence and competition between Nosema apis and Nosema ceranae in honey bees (Apis mellifera)

2015 ◽  
Vol 125 ◽  
pp. 9-15 ◽  
Author(s):  
Meghan O. Milbrath ◽  
Toan van Tran ◽  
Wei-Fong Huang ◽  
Leellen F. Solter ◽  
David R. Tarpy ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Nosema Ceranae ◽  
Nosema Apis
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