Evaluation of In Vitro Angiotensin-Converting Enzyme Inhibitory Activity of Psidium Guajava Linn. Leaf Extract and A Study on Enzyme Kinetics

2012 ◽  
Vol 1 (6) ◽  
pp. 1-2
Author(s):  
Jomon Sebastian ◽  
◽  
Jency Blesson
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Enzyme Kinetics ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Converting Enzyme

scholarly journals Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 844
Author(s):  
Razia Sultana ◽  
Adeola M. Alashi ◽  
Khaleda Islam ◽  
Md Saifullah ◽  
C. Emdad Haque ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Inhibitory Activity ◽  
The Other ◽  
Converting Enzyme ◽  
Inhibitory Activities ◽  
Ash Gourd ◽  
Better Than

The aim of the study was to determine the in vitro enzyme inhibition activities of aqueous polyphenolic extracts of nine popular Bangladeshi vegetables, namely ash gourd, bitter gourd, brinjal, Indian spinach, kangkong, okra, ridge gourd, snake gourd, and stem amaranth. Polyphenolic glycosides were the major compounds present in the extracts. Inhibition of α-amylase (up to 100% at 1 mg/mL) was stronger than α-glucosidase inhibition (up to 70.78% at 10 mg/mL). The Indian spinach extract was the strongest inhibitor of pancreatic lipase activity (IC50 = 276.77 µg/mL), which was significantly better than that of orlistat (381.16 µg/mL), a drug. Ash gourd (76.51%), brinjal (72.48%), and snake gourd (66.82%) extracts were the most effective inhibitors of angiotensin-converting enzyme (ACE), an enzyme whose excessive activities have been associated with hypertension. Brinjal also had a significantly higher renin-inhibitory activity than the other vegetable extracts. We conclude that the vegetable extracts may have the ability to reduce enzyme activities that have been associated with hyperglycemia, hyperlipidemia, and hypertension.

scholarly journals African locust bean (Parkia biglobosa, Jacq Benth) leaf extract affects mitochondrial redox chemistry and inhibits angiotensin-converting enzyme in vitro

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Kayode Komolafe ◽  
Afolabi C. Akinmoladun ◽  
Titilope R. Komolafe ◽  
Mary T. Olaleye ◽  
Akintunde A. Akindahunsi ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Leaf Extract ◽  
Redox Chemistry ◽  
Converting Enzyme ◽  
Parkia Biglobosa ◽  
Locust Bean ◽  
African Locust Bean

Chocolate desserts with ricotta hydrolysates: In vitro study of inhibitory activity against angiotensin‐converting enzyme and cholinesterase

2020 ◽  
Vol 85 (10) ◽  
pp. 3003-3011
Author(s):  
Joanna Kobus‐Cisowska ◽  
Oskar Szczepaniak ◽  
Daria Szymanowska ◽  
Monika Przeor ◽  
Maciej Jarzębski ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Converting Enzyme

scholarly journals Flavonoid-Rich Extract of Actinidia macrosperma (A Wild Kiwifruit) Inhibits Angiotensin-Converting Enzyme In Vitro

Foods ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 146 ◽  
Author(s):  
Sujeewa Hettihewa ◽  
Yacine Hemar ◽  
H. Rupasinghe
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
Epigallocatechin Gallate ◽  
Kinetic Studies ◽  
Converting Enzyme ◽  
Ace Inhibitory Activity ◽  
Fruit Samples ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Ace Inhibitory

Increasing interest in flavonoids in kiwifruit is due to the health-promoting properties of these bioactives. Inhibition of the angiotensin-converting enzyme (ACE) is one of the main therapeutic targets in controlling hypertension. The present study investigated the ACE inhibitory activity of flavonoid-rich extracts obtained from different kiwifruit genotypes. The flavonoid-rich extracts were prepared from fruits of Actinidia macrosperma, Actinidia deliciosa cv Hayward (Green kiwifruit), and Actinidia chinensis cv Hort 16A (Gold kiwifruit) by steeping the lyophilized fruit samples in 70% aqueous acetone, followed by partitioning the crude extracts with hexane. The composition of each extract was analyzed using ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). The ACE inhibitory activity of the fruit extracts was performed using a fluorescence-based biochemical assay. The subclass flavonol was the most abundant group of flavonoids detected in all the extracts tested from three different kiwifruit cultivars. Quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, quercetin-3-O-rutinoside, quercetin-3-O-arabinoglucoside, catechin, epigallocatechin gallate, epigallocatechin, chlorogenic, ferulic, isoferulic, and caffeic acid were prominent phenolics found in A. macrosperma kiwifruit. Overall, the flavonoid-rich extract from A. macrosperma showed a significantly (p < 0.05) high percentage of inhibition (IC50 = 0.49 mg/mL), and enzyme kinetic studies suggested that it inhibits ACE activity in vitro. The kiwifruit extracts tested were found to be moderately effective as ACE inhibitors in vitro when compared to the other plant extracts reported in the literature. Further studies should be carried out to identify the active compounds from A. macrosperma and to validate the findings using experimental animal models of hypertension.

Sign in / Sign up

Export Citation Format

Share Document