scholarly journals 5-HT2A, 5-HT1B/D, 5HT3 and 5-HT7 receptors as mediators of serotonin-induced direct contractile response of bovine airway smooth muscle

2021 ◽  
Vol 57 (0) ◽  
pp. 79-93
Author(s):  
Darwin Da Costa Guevara ◽  
Ernesto Trejo
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Contractile Response

Effects of mucosal removal on guinea-pig airway smooth muscle responsiveness

1986 ◽  
Vol 70 (6) ◽  
pp. 571-575 ◽  
Author(s):  
Christopher Murlas
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Electrical Field Stimulation ◽  
Physiological Salt Solution ◽  
Induced Responses ◽  
Field Stimulation ◽  
Electrical Field ◽  
Airway Mucosa

1. The contractile response to histamine, acetylcholine (ACh), KCl or electrical field stimulation (EFS) was examined in paired tracheal rings (one of each being denuded by mucosal rubbing), which were mounted in muscle chambers filled with a continuously aerated physiological salt solution at 37°C. 2. Removal of the respiratory mucosa increased the sensitivity of airway muscle to ACh, histamine and EFS, but not to KCl. The hypersensitivity of denuded rings to histamine and EFS was greater than to ACh. Atropine reduced the histamine hypersensitivity observed. 3. Pretreating intact preparations with indomethacin augmented their responsiveness to EFS, histamine and ACh. 4. Indomethacin augmentation of histamine- and EFS-induced responses was greater in preparations without epithelium. 5. We conclude that the airway mucosa may be associated with a factor that reduces airway smooth muscle responsiveness to stimulation.

Combined Effect of Isoproterenol and Mechanical Oscillation on the Contractile Response of Mice Airway Smooth Muscle From Healthy and Asthmatic Subjects

2012 ◽  
Author(s):  
M. J. Jo-Avila ◽  
A. M. Al-Jumaily ◽  
J. Lu ◽  
L. Sobrevia
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Healthy Subjects ◽  
Contractile Response ◽  
Relaxation Response ◽  
Driving Mechanism ◽  
Airway Lumen ◽  
Mechanical Oscillations ◽  
Normal Breathing ◽  
Asthma Attack

The main driving mechanism during an asthma attack is the hyperconstriction of airway smooth muscle (ASM), which reduces the airway lumen and makes normal breathing difficult. The contraction can be relieved by using bronchodilator drugs such as Isoproterenol (ISO). This paper hypothesizes that mechanical oscillations may improve drug therapy when combined with ISO or used alone in asthmatic subjects. Preliminary results indicate that combining ISO with breathing equivalent mechanical oscillations tends to increase the relaxation response, as compared to ISO alone in airways from healthy subjects, but not in the same manner in asthmatic airways. The effect of superposed oscillations of 1% and 1.5% amplitude in the range 5–20 Hz applied over breathing equivalent mechanical oscillations was also assessed in the study.

Specific reaginic antibody IgG1-induced changes of airway smooth muscle cells

1988 ◽  
Vol 65 (2) ◽  
pp. 767-775 ◽  
Author(s):  
M. Souhrada ◽  
J. F. Souhrada
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Isometric Force ◽  
Airway Smooth Muscle Cells ◽  
Base Line ◽  
Dependent Relationship ◽  
Reaginic Antibody ◽  
Induced Changes ◽  
Igg1 Concentration

It was found that 1) an administration of both immunoglobulin G1 (IgG1) or immunized serum caused an immediate depolarization and an increase in the isometric force of airway smooth muscle (ASM) cells, followed by a sustained hyperpolarization and a return of the tone to the base-line values; 2) an IgG1 concentration-dependent relationship was found between a peak depolarization, a peak hyperpolarization, and a peak isometric force; for these events 50% effective dose (ED50) was found to be 0.17, 0.14, and 0.25 microgram/ml of IgG1, respectively; 3) both electrical and contractile responses to ovalbumin of ASM cells sensitized with IgG1 were also dependent on the concentration of IgG1; the ED50 values of this relationship were 0.27 and 0.25 micrograms/ml of IgG1, respectively; 4) amiloride (10(-8) to 10(-5) M) pretreatment and a sodium-deficient environment attenuated sensitized-induced electrical and contractile changes as well as the response of ASM to ovalbumin (0.1%); and 5) pretreatment of ASM with diphenhydramine (10(-5) M) or FPL 55712 (10(-6) M) had no effect on sensitization-induced changes in membrane potential but attenuated electrical and contractile response of ASM to ovalbumin (0.1%).

Study of the Combined Effect of Isoproterenol and Mechanical Oscillation on the Contractile Response of Mice Airways From Healthy and Asthmatic Subjects (In Vivo)

2013 ◽  
Author(s):  
M. Jo-Avila ◽  
A. Al-Jumaily ◽  
J. Lu ◽  
L. Sobrevia
Keyword(s):  
Smooth Muscle ◽  
Drug Therapy ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Combined Effect ◽  
Driving Mechanism ◽  
Airway Lumen ◽  
Normal Breathing ◽  
Asthma Attack

The main driving mechanism during an asthma attack is the hyperconstriction of airway smooth muscle (ASM), which reduces the airway lumen and makes normal breathing difficult. The contraction can be relieved by using bronchodilator drugs such as Isoproterenol (ISO). This paper hypothesizes that superimposed length oscillations (SILO) may improve drug therapy when combined with ISO or used alone in asthmatic subjects. The aim of this study is to assess SILO patterns directly onto the airways of healthy and asthmatic subjects (mice), while they are under anaesthesia breathing spontaneously and pre-constricted (mimicking and asthmatic attack), and compared the response with the relaxation observed just with ISO.

SP-induced contraction of airway smooth muscle in normal and allergen-sensitized rabbits: mechanism of action

1995 ◽  
Vol 78 (2) ◽  
pp. 428-432 ◽  
Author(s):  
G. N. Colasurdo ◽  
J. E. Loader ◽  
J. P. Graves ◽  
G. L. Larsen
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Indirect Effects ◽  
Contractile Response ◽  
Complex Interaction ◽  
Direct Effects ◽  
Response Curves ◽  
Cholinergic Neurotransmission ◽  
The Right

We studied the mechanisms involved in the airway smooth muscle (ASM) contraction to substance P (SP) in normal (control) and allergen-sensitized (immune) rabbits as well as immune rabbits exposed to allergen via the airways (immune challenged). Cumulative concentration-response curves to SP (1 x 10(-9) to 1 x 10(-4) M) were performed in ASM segments in the absence and presence of atropine (10(-5) M) in vitro. The maximal contractile response (g tension/g tissue) at 10(-4) M SP and ASM contractions at various concentrations of SP were expressed as means +/- SE. We found no difference in the contractile response to SP between control and immune animals. ASM segments obtained from immune-challenged rabbits were more responsive to SP. Atropine shifted to the right the concentration-response curves and decreased the maximal ASM contraction at 10(-4) M SP in all three groups; this effect, however, was greater in immune-challenged tissues. These findings demonstrate an increased contractile response to SP in immune-challenged animals mediated by a more pronounced facilitation of cholinergic neurotransmission. We conclude that the final ASM response to SP is the result of a complex interaction between direct effects on ASM and indirect effects through modulation of cholinergic neurotransmission.

Influence of hydroxyl radical on rabbit airway smooth muscle chronically exposed to H2O2 in vivo

1993 ◽  
Vol 264 (6) ◽  
pp. L566-L574 ◽  
Author(s):  
K. Prasad ◽  
J. B. Gupta
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Smooth Muscles ◽  
Relaxant Effect ◽  
Arachidonic Acid Metabolites ◽  
Acid Metabolites ◽  
Small Contraction ◽  
Epithelial Dysfunction

The effects of .OH on the isolated tracheal smooth muscles (TSM), from control, polyethylene glycol (PEG)-glucose oxidase (GO)-, and GO+PEG catalase-treated rabbits were investigated. GO or GO+catalase were given intravenously each week for 4 mo. .OH produced relaxation of basal and ACh-precontracted tension in TSM of control rabbits. The relaxant effect was attenuated by removal of epithelium, whereas it was converted to contraction in the indomethacin-pretreated muscle. .OH produced contraction of TSM and ACh-precontracted muscle in GO-treated rabbits. The contractile response was abolished in preparations denuded of epithelium or pretreated with indomethacin. .OH produced relaxation in basal tension, but a small contraction in ACh-precontracted muscle of GO+catalase-treated rabbits. The contractile response to .OH was unaffected by indomethacin pretreatment; however, it was converted to relaxation in the preparations denuded of epithelium. Contractile response of TSM to ACh was augmented in deepithelialized, indomethacin-, or GO-treated preparations. H2O2 damaged the tracheal epithelium. These results suggest that 1) .OH-induced relaxation/contraction of TSM is partly epithelium dependent and is mediated by bronchodilator/bronchoconstrictor arachidonic acid metabolites, 2) the airway smooth muscle with healthy epithelium responds to .OH differently from those with dysfunctional or damaged epithelium, and 3) hyperresponsiveness of the airways to ACh may be related to the epithelial dysfunction.

Heme oxygenase modulates oxidant-signaled airway smooth muscle contractility: role of bilirubin

2002 ◽  
Vol 283 (3) ◽  
pp. L596-L603 ◽  
Author(s):  
Abdoulaye Samb ◽  
Camille Taillé ◽  
Abdelhamid Almolki ◽  
Jérôme Mégret ◽  
James M. Staddon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Heme Oxygenase ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Contractile Response ◽  
Redox Status ◽  
Ros Production ◽  
Smooth Muscle Contractility

Reactive oxygen species (ROS) increase the contractile response of airway smooth muscle (ASM). Heme oxygenase (HO) catabolizes heme to the powerful antioxidant bilirubin. Because HO is expressed in the airways, we investigated its effects on ASM contractility and ROS production in guinea pig trachea. HO expression was higher in the epithelium than in tracheal smooth muscle. Incubation of tracheal rings (TR) with the HO inhibitor tin protoporphyrin (SnPP IX) or the HO substrate hemin increased and decreased, respectively, ASM contractile response to carbamylcholine. The effect of hemin was reversed by SnPP and mimicked by the antioxidants superoxide dismutase (SOD) and catalase. Hemin significantly reduced the effect of carbamylcholine in rings treated with the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ), compared with ODQ-treated rings without hemin incubation, suggesting that the CO-guanosine 3′,5′-cyclic monophosphate pathway was not involved in the control of tracheal reactivity. SnPP and hemin increased and decreased ROS production by TR by 18 and 38%, respectively. Bilirubin (100 pM) significantly decreased TR contractility and ROS production. Hemin, bilirubin, and SOD/catalase decreased phosphorylation of the contractile protein myosin light chain, whereas SnPP significantly augmented it. These data suggest that modulation of the redox status by HO and, moreover, by bilirubin modulates ASM contractility by modulating levels of phosphorylated myosin light chain.

Combined Effect of Isoproterenol and Mechanical Oscillation on the Contractile Response of Mice Airway Smooth Muscle

2011 ◽  
Author(s):  
M. J. Jo-Avila ◽  
A. M. Al-Jumaily ◽  
P. Mbikou ◽  
L. Sobrevia
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Combined Effect ◽  
Relaxation Response ◽  
Preliminary Results ◽  
Mechanical Oscillation ◽  
Asthmatic Attack ◽  
Mechanical Oscillations

An asthmatic attack is instigated by hyperconstriction of airway smooth muscle (ASM). This can be relieved with bronchodilator medication such as Isoproterenol (ISO). This paper hypothesizes that mechanical oscillations may improve drugs therapy when combined with ISO. Preliminary results indicate that combining ISO with breathing equivalent mechanical oscillations trends to increase the relaxation response as compared with the use of ISO alone. The effect of superposed oscillations in the range of 10–30 Hz of frequency applied over breathing equivalent mechanical oscillations was also assessed in the study.

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