Influence of Ways to Thaw Bull Sperm on Its Quality

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

Download Full-text

DeepSperm: A robust and real-time bull sperm-cell detection in densely populated semen videos

Computer Methods and Programs in Biomedicine ◽

10.1016/j.cmpb.2021.106302 ◽

2021 ◽

pp. 106302

Author(s):

Priyanto Hidayatullah ◽

Xueting Wang ◽

Toshihiko Yamasaki ◽

Tati L.E.R. Mengko ◽

Rinaldi Munir ◽

...

Keyword(s):

Real Time ◽

Sperm Cell ◽

Cell Detection ◽

Bull Sperm

Download Full-text

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

Reproduction in Domestic Animals ◽

10.1111/rda.13748 ◽

2020 ◽

Vol 55 (9) ◽

pp. 1103-1114

Author(s):

Pei‐Pei Zhang ◽

Jing‐Jing Wang ◽

Chong‐Yang Li ◽

Hai‐sheng Hao ◽

Hao‐Yu Wang ◽

...

Keyword(s):

Oxidative Stress ◽

In Vitro Fertilization ◽

Vitamin C ◽

Protective Effect ◽

Vitro Fertilization ◽

Bull Sperm ◽

Fertilization Capacity

Download Full-text

Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility

Theriogenology ◽

10.1016/s0093-691x(98)00036-3 ◽

1998 ◽

Vol 49 (4) ◽

pp. 871-879 ◽

Cited By ~ 151

Author(s):

P.B. Farrell ◽

G.A. Presicce ◽

C.C. Brockett ◽

R.H. Foote

Keyword(s):

Computer Assisted ◽

Sperm Analysis ◽

Sperm Characteristics ◽

Computer Assisted Sperm Analysis ◽

Bull Sperm ◽

The Relationship

Download Full-text

Effect of sex-sorting on the ability of fresh and cryopreserved bull sperm to undergo an acrosome reaction

Theriogenology ◽

10.1016/j.theriogenology.2006.01.063 ◽

2006 ◽

Vol 66 (4) ◽

pp. 929-936 ◽

Cited By ~ 41

Author(s):

Eva Mocé ◽

James K. Graham ◽

John L. Schenk

Keyword(s):

Acrosome Reaction ◽

Bull Sperm

Download Full-text

Wirkung von Forskolin auf die Spermienmotilität. in-vitro-Versuche unter Verwendung von Bullensperma/The Effect of Forskolin on Sperm Motility. In Vitro Experiments with Bull Sperm.

Andrologia ◽

10.1111/j.1439-0272.1989.tb02413.x ◽

2009 ◽

Vol 21 (3) ◽

pp. 293-296

Author(s):

U. Fuchs ◽

H.-H. Riedel

Keyword(s):

Sperm Motility ◽

In Vitro Experiments ◽

Bull Sperm

Download Full-text

The stirring of the medium by bull spermatozoa

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1963.0022 ◽

1963 ◽

Vol 157 (969) ◽

pp. 461-472 ◽

Cited By ~ 4

Keyword(s):

Diffusion Coefficient ◽

Short Range ◽

Short Range Order ◽

Effective Diffusion ◽

Sperm Head ◽

Special Shape ◽

Bull Semen ◽

Sperm Density ◽

Bull Sperm ◽

Diffusion Coefficient Of Oxygen

1. Bull semen diluted 1/5 or 1/10 respired at the same rate whether the manometers were stationary or shaken. 2. Respirometric experiments using a manometer flask of special shape showed that bull sperm suspensions achieve this result by increasing the effective diffusion coefficient of oxygen in the suspending medium by 900%. 3. The hypothesis is put forward ( a ) that these results are caused by the existence of short-range order, as opposed to disorder, in bull sperm suspensions, even at comparatively low sperm densities (dilution 1/8 to 1/20); ( b ) that this order produces group sperm velocities greater than those of isolated spermatozoa; and ( c ) that as a result, larger volumes of the suspending medium are convected with the ordered sperm groups, causing an augmented ‘diffusion’ of oxygen. 4. This hypothesis was examined by taking photomicrographs of sperm suspensions at different dilutions and temperatures and determining from them the distributions of (i) the distance between pairs of nearest spermatozoa; (ii) the angle of inclination of a sperm head relative to that of the spermatozoon nearest to it; and (iii) the relative position of the nearest spermatozoon. 5. Comparison of the observed distributions and the corresponding random ones showed that the spermatozoa attracted each other, so that transient sperm groups were formed, in which the spermatozoa tended to swim in the same direction. A reduction in temperature or sperm density decreased the sperm order.

Download Full-text