Investigation of seed storage protein and bread-making quality of triticale

2000 ◽  
Vol 48 (1) ◽  
pp. 41-50 ◽  
Author(s):  
M. Tohver ◽  
R. Täht ◽  
I. Rahnu ◽  
A. Kann
Keyword(s):  
Seed Storage Protein ◽  
Seed Storage ◽  
Subunit Composition ◽  
Whole Grain ◽  
Bread Making ◽  
Winter Triticale ◽  
Sds Page ◽  
Hmw Glutenin ◽  
Whole Grain Flour

The subunit composition of reduced prolamin fractions of winter triticale cultivars of different pedigrees was investigated by means of SDS-PAGE. A comparative analysis of the electrophoregram patterns of triticale cultivars and those of wheat and rye revealed that each cultivar had a unique pattern. Both the glutenin subunits of wheat and the secalin units of rye could be found in triticale cultivars. Whole-grain flour of triticale and wheat was used in baking experiments. The HMW glutenin subunit composition and baking quality of triticale cultivars were in the predicted correlation. Flour from the triticale cultivars could be used in breadmaking by mixing it with up to 30% wheat flour.

scholarly journals Variation in HMW glutenin subunits of different species of wheat

2011 ◽  
Vol 48 (No. 1) ◽  
pp. 15-19
Author(s):  
Z. Gálová ◽  
MichalíkI ◽  
H. Knoblochová ◽  
E. Gregová
Keyword(s):  
Triticum Turgidum ◽  
Wheat Cultivars ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Varieties ◽  
Hmw Glutenin Subunits ◽  
Sds Page ◽  
Hmw Glutenin ◽  
Triticum Durum Desf

Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat.

HMW glutenin subunit composition and bread making quality of Austrian grown wheats

1997 ◽  
Vol 25 (4) ◽  
pp. 955-962 ◽  
Author(s):  
Sabine Gröger ◽  
M. Oberforster ◽  
M. Werteker ◽  
H. Grausgruber ◽  
T. Lelley
Keyword(s):  
Glutenin Subunit ◽  
Subunit Composition ◽  
Bread Making ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin

scholarly journals Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Mohammad Javad Zamani ◽  
Mohammad Reza Bihamta ◽  
Behnam Naserian Khiabani ◽  
Zahra Tahernezhad ◽  
Mohammad Taher Hallajian ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Allelic Diversity ◽  
Seed Storage ◽  
D Genome ◽  
Baking Quality ◽  
Dough Quality ◽  
Sds Page ◽  
Hmw Glutenin

Allelic diversity of HMW glutenin loci in several studies revealed that allelic combinations affect dough quality. Dx5 + Dy10 subunits are related to good baking quality and Dx2 + Dy12 are related to undesirable baking quality. One of the most regular methods to evaluate the baking quality is SDS-PAGE which is used to improve baking quality labs. Marker-assisted selection is the method which can recognize the alleles related to baking quality and this method is based on polymerase chain reaction. 10 pairs of specific primers related to Dx2, Dx2.1, Dx5, Dy10, and Dy12 subunits were used for recognizing baking quality of some wheat varieties and some mutant genotypes. Only 5 pairs of them could show the specific bands. All subunits were recognized by the primers except Dx2.1. Some of the primers were extracted from previous studies and the others were designed based on D genome subunits of wheat. SDS-PAGE method accomplished having confidence in these marker’s results. To realize the effect of mutation, seed storage proteins were measured. It showed that mutation had effect on the amount of seed storage protein on the mutant seeds (which showed polymorphism).

scholarly journals Characteristics of Seed Storage Protein Affecting the Eating Quality of Japonica and Tongil-type Rice

2016 ◽  
Vol 61 (4) ◽  
pp. 227-234
Author(s):  
Jieun Kwak ◽  
◽  
Jeom-Sig Lee ◽  
Mi-Ra Yoon ◽  
Mi-Jung Kim ◽  
...  
Keyword(s):  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Eating Quality

Comparative Bread Quality of White Flour and Whole Grain Flour for Hard Red Spring and Winter Wheat

Crop Science ◽  
2001 ◽  
Vol 41 (6) ◽  
pp. 1917-1920 ◽  
Author(s):  
P. L. Bruckner ◽  
D. Habernicht ◽  
G. R. Carlson ◽  
D. M. Wichman ◽  
L. E. Talbert
Keyword(s):  
Winter Wheat ◽  
Bread Quality ◽  
Whole Grain ◽  
White Flour ◽  
Whole Grain Flour

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

The Electrophoretic Contribution to the Solutions of Taxonomical Problems of Some Lathyrus L. Taxa Growing in Turkey

2020 ◽  
Author(s):  
H. Genc ◽  
İ. Emre ◽  
A. Sahin
Keyword(s):  
Seed Storage Protein ◽  
Extraction Procedure ◽  
Seed Proteins ◽  
Seed Storage ◽  
Morphological Data ◽  
Protein Patterns ◽  
Documentation System ◽  
Natural Habitats ◽  
Protein Assay ◽  
Sds Page

Background: The seed proteins are used as molecular markers to clarify the systematic problems. Also, electrophoretic techniques are safe tools to identify the seed proteins. In present study, it was used the SDS-PAGE technique to solve the taxonomical problems of eight taxa of genus Lathyrus belong to three sections Orobus, Lathyrostylis and Pratensis according to the globulin B and glutelin. Methods: The seed materials were collected from natural habitats and 0.5 g seed were homogenized and centrifuged based on extraction procedure. In addition, the amounts of seed storage protein subfractions were determined by using protein assay. The electrophoretic analysis were performed according to the 12% SDS-PAGE. The gel documentation system (Bio-Rad, USA) was used to analyse the electrophoretic data and UPGAMA was used to construct the dendogram to show the relationships among the species under focus. Results: Current study showed that the species of section Pratensis were different from species of sections Orobus and Lathyrostylis. The differences among the studied taxa shown clearly and all studied taxa were choosen from the protein patterns. Furthermore, present study demonstrated that L. nivalis has the highest globulin B and glutelin. Results of the present study generally supported the morphological data.

scholarly journals Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice

2013 ◽  
Vol 14 (8) ◽  
pp. 17073-17084 ◽  
Author(s):  
Hyun-Jung Kim ◽  
Jong-Yeol Lee ◽  
Ung-Han Yoon ◽  
Sun-Hyung Lim ◽  
Young-Mi Kim
Keyword(s):  
Storage Protein ◽  
Nutritional Quality ◽  
Seed Storage Protein ◽  
Protein Composition ◽  
Seed Storage
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