Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells: Short communication
2002 ◽
Vol 50
(2)
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pp. 211-215
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Keyword(s):
Rate of amiloride-sensitive Na+ uptake into cultured rumen epithelial cells was studied in order to clarify the influence of culture conditions on Na+/H+ exchange (NHE). Cell cultures were exposed to Na-n-butyrate or not for seven days or subcultured. On the 14th day of culturing, primary cell cultures without butyrate exposure showed both non-stratified and stratified growth. Na-n-butyrate treated 14-day-old cultures and 3-day-old subcultures contained mostly non-stratified, i.e. non-keratinised cells. Both n-butyrate treatment and subculturing increased total and amiloride-sensitive Na+ uptake. Our results indicate that Na+ uptake via NHE is determined by the amount and the ratio of non-stratified (non-keratinised) cells.
2006 ◽
Vol 70
(1-2)
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pp. 1-19
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Keyword(s):
1989 ◽
Vol 224
(4)
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pp. 465-470
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Keyword(s):
1988 ◽
Vol 405
(1)
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pp. 77-103
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Keyword(s):
Keyword(s):
2013 ◽
Vol 50
(2)
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pp. 139-145
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1999 ◽
Vol 35
(10)
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pp. 593-598
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Keyword(s):
1988 ◽
Vol 263
(23)
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pp. 11504-11510
Keyword(s):
Keyword(s):