Response of spring barley cultivars to Ramularia leaf spot in conditions of the Czech Republic

2013 ◽  
Vol 41 (1) ◽  
pp. 126-132 ◽  
Author(s):  
P. Matušinsky ◽  
M. Hanusová ◽  
L. Stemberková ◽  
P. Mařík ◽  
V. Minaříková ◽  
...  
2011 ◽  
Vol 47 (No. 1) ◽  
pp. 37-40 ◽  
Author(s):  
P. Mařík ◽  
Z. Šnejdar ◽  
P. Matušinsky

The fungus Ramularia collo-cygni is increasingly important as the causal agent of Ramularia leaf spot (RLS), a novel leaf spot disease of barley. The work aimed to identify gene resources suitable for developing new breeding lines of winter barley with improved resistance. During the first experimental period (2001–2005), RLS incidence was monitored in 711 cultivars and advanced breeding lines. Differences were detected in the intensity of symptomatic expression, but no material showed high resistance. During the second experimental period (2006–2009), response to natural RLS infection was evaluated in 19 winter barley cultivars (12 six-row and 7 two-row) registered in the Czech Republic. Highest susceptibility was detected in 6-row cvs. Luran, Laverda and Wendy while cvs Breunskylie (2-row), Merlot and Highlight (both 6-row) showed relatively lower disease incidence. High resistance was not detected. On average, 2-row cultivars showed lower intensity than 6-row cultivars and significant variation was observed among years.


2010 ◽  
Vol 40 (No. 4) ◽  
pp. 135-140 ◽  
Author(s):  
A. Dreiseitl

The virulence frequencies to powdery mildew resistance genes possessed by winter barley cultivars registered and newly tested in the Czech Republic were studied in 2000, 2002 and 2004. Random samples of the populations originating from winter and spring barley fields were obtained from the air by a mobile version of a jet spore sampler mounted on a car roof. Conidia were sampled by driving across the Czech Republic. Fourteen differentials, carrying 18 out of 20 currently identified resistance genes present in winter barley cultivars, were used. High virulence frequencies (85–100%) to most resistance genes were found. Lower virulence frequencies (14.1–40.1%) were found to only three resistance genes that have not been described yet; their preliminary designations are Ml(Va), Ml(Dt) and Ml(Ca). The importance of resistance of winter barley cultivars is discussed with respect to limiting the speed with which the pathogen adapts to genetic resistances possessed by commercial cultivars of both winter and spring barley, and to the necessity of lowering the costs for powdery mildew control in barley.


2012 ◽  
Vol 47 (No. 2) ◽  
pp. 43-51 ◽  
Author(s):  
A. Dreiseitl

Virulences to powdery mildew resistances in barley cultivars mostly carrying unknown resistances were determined in 2009 and 2010. Random spore samples of the airborne pathogen populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler by travelling across theCzech Republic. In total 301 isolates were studied, 55 differentials carrying mostly unknown resistances were used and 80 pathotypes were found, of which 26 representing 73.1% of isolates were detected in both years. Virulence frequencies showed a wide range from 0% to 100%. Complexity of the 2010 population slightly increased, mostly due to increasing frequencies of virulence to new resistances, whereas the complexity of virulences to resistances in most other differentials decreased. Pathotype 00027 was the most abundant (10.0%). Diversity of the 2010 population  considerably increased due to changes in virulence frequencies.


2011 ◽  
Vol 50 (No. 10) ◽  
pp. 456-462 ◽  
Author(s):  
V. Šerhantová ◽  
J. Ehrenbergerová ◽  
L. Ohnoutková

Immature zygotic embryos are frequently used for induction of cell cultures in vitro and for genetic transformation. The effect of three synthetic auxins: 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropicolinic acid (picloram) on induction and subsequent regeneration capacity of barley. In vitro culture was studied in 12 spring barley cultivars registered in the Czech Republic and a variety of Golden Promise, with known high regeneration efficiency. The callus formation frequency and number of green regenerants were influenced significantly both by genotype and auxin. Most cultivars formed statistically significantly a higher mean number of regenerants (1.95) after the callus induction on the medium with 2.4-D as compared to the media with picloram and dicamba. Only two cultivars (Nordus and SG-S-261) did not respond to the used auxins differently. The highest average number of regenerants (from all three auxins) was obtained with Golden Promise (2.7 plants per one cultivated scutellum). From the set of genotypes used in our study, the cultivars Atribut, Forum, and Scarlett with the mean number of regenerants (2.09–1.57) would be the most suitable cultivars for further transformation use. They differ statistically significantly from the cultivars Nordus, Amulet, Akcent, SG-S-252, Orbit, and Granát (0.42–0.92) which had the lowest mean number of regenerants.


2009 ◽  
Vol 44 (No. 4) ◽  
pp. 160-166 ◽  
Author(s):  
A. Dreiseitl

Virulence frequencies to powdery mildew resistances in winter barley cultivars mostly registered in the Czech Republic were studied in 2007 and 2008. Random samples of the air populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler. Conidia were sampled by driving across the Czech Republic. In total 349 isolates were studied and 17 differentials were used. The virulence frequencies to specific resistances of given cultivars showed wide range from 0% to 100%. Nine differentials were used to distinguish 134 pathotypes, of which 32 representing 63.9% of isolates were detected in both years. Pathotype 773, which broke down the resistance of eight differentials, was the most abundant. In 2008, lower virulence frequencies to all differentials, and thus lower population complexity, were determined, which may be caused by different regional origins of the isolates examined. Importance of the study of the given pathogen population is discussed in terms of successful breeding of resistant barley cultivars.


2021 ◽  
Vol 256 ◽  
pp. 107064
Author(s):  
František Jurečka ◽  
Milan Fischer ◽  
Petr Hlavinka ◽  
Jan Balek ◽  
Daniela Semerádová ◽  
...  

2011 ◽  
Vol 47 (No. 2) ◽  
pp. 58-63 ◽  
Author(s):  
J. Chrpová ◽  
V. Šíp ◽  
L. Štočková ◽  
L. Stemberková ◽  
L. Tvarůžek

Fusarium head blight (FHB) is a fungal disease causing substantial yield and quality losses in barley. Genetic variation in deoxynivalenol (DON) content and and important yield traits in response to FHB were studied in 44 spring barley cultivars for two years following artificial inoculation with Fusarium culmorum under field conditions. The analysis of variance revealed that the largest effect on DON content and simultaneously on the reduction of thousand grain weight and grain weight per spike were due to the environmental conditions of the year, while the visual disease symptoms depended on the cultivars to a larger extent. All these traits were significantly interrelated. The most resistant cultivars Murasski mochi, Nordic, Krasnodarskij 35, Krasnodarskij 95, Nordus, and Usurijskij 8, together with the resistant check Chevron, showed the lowest DON content, the lowest expression of disease symptoms and the lowest reduction of TGW and GWS. However, most spring barley cultivars registered in the Czech Republic in recent years expressed susceptibility or medium resistance and were considerably affected by the disease. This increases the importance of breeding barley for resistance to FHB.


2014 ◽  
Vol 50 (No. 1) ◽  
pp. 8-16 ◽  
Author(s):  
J. Poslušná ◽  
E. Plachká

In the period 2009&ndash;2011 monitoring of the incidence of phoma stem canker was carried out at selected sites of the Czech Republic (&Scaron;umperk and Opava regions in North Moravia). The risk of infection was evaluated by assessing the autumn release of&nbsp;Leptosphaeria&nbsp;spp. ascospores and with the proPlant prediction model. In recent years, the incidence of phoma stem canker has been relatively low and this corresponded with the total count of ascospores released in the autumn period but not with an increased level of infection risk announced by the proPlant model. During the monitored period the ascospore concentration reached maximally 2 ascospores/1 m<sup>3</sup>&nbsp;per day. Maximum ascospore release was recorded in 2010 as a result of favourable weather conditions. The first incidence of phoma leaf spot has been observed in mid-October. The higher values of ascospores concentration were recorded in the Opava region, but the total number of the trapped ascospores was more often higher in the &Scaron;umperk region.&nbsp;


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