scholarly journals Forecasting system for infection risk of phoma stem canker in selected regions of the Czech Republic in 2009–2011 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 8-16 ◽  
Author(s):  
J. Poslušná ◽  
E. Plachká

In the period 2009&ndash;2011 monitoring of the incidence of phoma stem canker was carried out at selected sites of the Czech Republic (&Scaron;umperk and Opava regions in North Moravia). The risk of infection was evaluated by assessing the autumn release of&nbsp;Leptosphaeria&nbsp;spp. ascospores and with the proPlant prediction model. In recent years, the incidence of phoma stem canker has been relatively low and this corresponded with the total count of ascospores released in the autumn period but not with an increased level of infection risk announced by the proPlant model. During the monitored period the ascospore concentration reached maximally 2 ascospores/1 m<sup>3</sup>&nbsp;per day. Maximum ascospore release was recorded in 2010 as a result of favourable weather conditions. The first incidence of phoma leaf spot has been observed in mid-October. The higher values of ascospores concentration were recorded in the Opava region, but the total number of the trapped ascospores was more often higher in the &Scaron;umperk region.&nbsp;

2017 ◽  
Vol 68 (3) ◽  
pp. 254 ◽  
Author(s):  
Jana Mazáková ◽  
Jan Urban ◽  
Miloslav Zouhar ◽  
Pavel Ryšánek

In the Czech Republic, increases in the area sown with oilseed rape (Brassica napus L.) and shifts to intensive crop farming systems have contributed to increased incidence and harmfulness of phoma stem canker. This study comprised a large-scale survey of the occurrence of two closely related causal agents of the disease, Leptosphaeria maculans and L. biglobosa, in oilseed rape tissues and the country-wide distribution of each fungal species. In the 2007–11 growing seasons, 1132 leaves with phoma leaf spot symptoms were sampled; from those, 977 L. maculans-type and 477 L. biglobosa-type leaf spots were sampled and analysed by species-specific PCR without pathogen isolation. There were 1159 leaf spots confirmed as infected by Leptosphaeria spp., with 65% of 907 L. maculans-type leaf spots infected by L. maculans only and 35% co-infected by both species; and with 88% of 252 L. biglobosa-type leaf spots infected by L. biglobosa only and 12% co-infected by both species. Furthermore, 217 monopycnidial isolates were collected from selected leaf spots and identified based on pigment production during solid- and liquid-media culture and PCR assay. Most (82%) isolates originating from L. maculans-type leaf lesions were L. maculans, and most (69%) isolates collected from L. biglobosa-type leaf lesions were L. biglobosa. Co-infection by both species was found in both L. maculans-type and L. biglobosa-type leaf lesions. In 2007–12, 708 stems with phoma stem canker symptoms and 2635 plant tissues from upper stem, stem base, root collar and taproot of each stem were sampled for PCR; symptoms on the four parts of each stem were assessed before taking tissue samples. There were 1495 plant tissues confirmed as infected by Leptosphaeria spp., with the proportion of plant tissue in which only L. biglobosa DNA (62%) was amplified greater than that with only L. maculans DNA (11%) or with both L. maculans and L. biglobosa DNA (27%). Although both species were detected in leaf samples in autumn, L. biglobosa was the more frequently detected species in stem samples in summer, suggesting that L. biglobosa is the more successful in colonising oilseed rape tissues in later growth stages of the plant in the Czech Republic.


2014 ◽  
Vol 50 (No. 1) ◽  
pp. 17-18
Author(s):  
I. Šafránková

This is the first report of&nbsp;Puccinia oxalidis&nbsp;causing leaf spot diseases on ornamental&nbsp;Oxalis triangularis&nbsp;subsp.&nbsp;papilionaceae&nbsp;cv. Atropurpurea in Moravia, Czech Republic. The macroscopic symptoms and microscopic features are described.


2021 ◽  
Author(s):  
Rudolf Brázdil ◽  
Kateřina Chromá ◽  
Lukáš Dolák ◽  
Jan Řehoř ◽  
Ladislava Řezníčková ◽  
...  

Abstract. This paper presents an analysis of fatalities attributable to weather conditions in the Czech Republic during the 2000–2019 period. The database of fatalities deployed contains information extracted from Právo, a leading daily newspaper, and Novinky.cz, its internet equivalent, supplemented by a number of other documentary sources. The analysis is performed for floods, windstorms, convective storms, rain, snow, glaze ice, frost, heat, and fog. For each of them, the associated fatalities are investigated in terms of annual frequencies, trends, annual variation, spatial distribution, cause, type, place, and time, as well as the sex, age, and behaviour of casualties. There were 1164 weather-related fatalities during the 2000–2019 study period, exhibiting a statistically significant falling trend. Those attributable to frost (31 %) predominated, followed by glaze ice, rain and snow. Fatalities were at their maximum in January and December and at their minimum in April and September. Fatalities arising out of vehicle accidents (48 %) predominated in terms of structure, followed by freezing or hypothermia (30 %). Most deaths occurred during the night. Adults (65 %) and males (72 %) accounted for the majority of fatalities, while indirect fatalities were more frequent than direct ones (55 % to 45 %). Hazardous behaviour accounted for 76 %. According to the database of the Czech Statistical Office, deaths caused by exposure to excessive natural cold are markedly predominant among five selected groups of weather-related fatalities and their numbers exhibit a statistically significant rise during 2000–2019. Police yearbooks of the fatalities arising out of vehicle accidents indicate significantly decreasing trends in the frequency of inclement weather patterns associated with fatal accidents, as well as a decrease in their percentage in annual numbers of fatalities. The discussion of results includes the problems of data uncertainty, comparison of different data sources, and the broader context.


2021 ◽  
Vol 21 (5) ◽  
pp. 1355-1382
Author(s):  
Rudolf Brázdil ◽  
Kateřina Chromá ◽  
Lukáš Dolák ◽  
Jan Řehoř ◽  
Ladislava Řezníčková ◽  
...  

Abstract. This paper presents an analysis of fatalities attributable to weather conditions in the Czech Republic during the 2000–2019 period. The database of fatalities deployed contains information extracted from Právo, a leading daily newspaper, and Novinky.cz, its internet equivalent, supplemented by a number of other documentary sources. The analysis is performed for floods, windstorms, convective storms, rain, snow, glaze ice, frost, heat, and fog. For each of them, the associated fatalities are investigated in terms of annual frequencies, trends, annual variation, spatial distribution, cause, type, place, and time as well as the sex, age, and behaviour of casualties. There were 1164 weather-related fatalities during the 2000–2019 study period, exhibiting a statistically significant falling trend. Those attributable to frost (31 %) predominated, followed by glaze ice, rain, and snow. Fatalities were at their maximum in January and December and at their minimum in April and September. Fatalities arising out of vehicle accidents (48 %) predominated in terms of structure, followed by freezing or hypothermia (30 %). Most deaths occurred during the night. Adults (65 %) and males (72 %) accounted for the majority of fatalities, while indirect fatalities were more frequent than direct ones (55 % to 45 %). Hazardous behaviour accounted for 76 %. According to the database of the Czech Statistical Office, deaths caused by exposure to excessive natural cold are markedly predominant among five selected groups of weather-related fatalities, and their numbers exhibit a statistically significant rise during 2000–2019. Police yearbooks of the fatalities arising out of vehicle accidents indicate significantly decreasing trends in the frequency of inclement weather patterns associated with fatal accidents as well as a decrease in their percentage in annual numbers of fatalities. The discussion of results includes the problems of data uncertainty, comparison of different data sources, and the broader context.


2008 ◽  
Vol 43 (No. 1) ◽  
pp. 10-12 ◽  
Author(s):  
I. Šafránková

Woody ornamental cover plants of Japanese pachysandra (<i>P. terminalis</i> S. et Z.) are planted in parks and gardens in the Czech Republic. A serious disease of these plants is Volutella leaf blight and stem canker caused by the fungus <i>Pseudonectria pachysandricola</i> (anamorph <i>Volutella pachysandricola</i>). It was described by DODGE (1944) in the United States and appeared in Europe in the 1980s. Volutella pachysandricola was isolated from Japanese pachysandra (<i>P. terminalis</i> cvs. Green Carpet and Variegata) from leaf spots and stem and stolon cankers in Brno in 2000&minus;2003. The tan or brown spots with brown margins, often with concentric zones, develop on infected leaves. Stem and stolon cankers appear as water-soaked diseased areas, the stem often turns brown, shrivels and girdles. The infection often begins in damaged or senescent plant parts and spreads into the healthy tissues. Pink-orange sporodochia with spores form on newly killed stems and leaves during humid spring and summer periods. Ascospores develop in red-orange perithecia on the same tissues.


2009 ◽  
Vol 45 (No. 1) ◽  
pp. 12-15
Author(s):  
I. Šafránková ◽  
J. Müller

A downy mildew was observed on <I>Buddleja × weyeriana</I> Weyer ex Rehd. (<I>B. davidii × globosa</I>) cv. Sungold in a nursery in the northern part of the Czech Republic in July 2008. This downy mildew disease of <I>Buddleja</I> (Butterfly Bush) had been unknown in the country so far. Characteristics of visual symptoms and microscopic features are described. The morphology of the fungus was typical for <I>Peronospora hariotii</I>, conforming to the published description by GÄUMANN (1919). The sexual stage was not found. This is the first report of <I>Peronospora hariotii </I>causing leaf spot disease on <I>Buddleja</I> in the Czech Republic.


2003 ◽  
Vol 31 (1-2) ◽  
pp. 145-151 ◽  
Author(s):  
Jana Šárová ◽  
Alena Hanzalová ◽  
Pavel Bartoš

Plant Disease ◽  
2012 ◽  
Vol 96 (3) ◽  
pp. 461-461 ◽  
Author(s):  
D. Šafářová ◽  
M. Navrátil ◽  
C. Faure ◽  
T. Candresse ◽  
A. Marais

Apricot pseudo-chlorotic leaf spot virus (APCLSV) is a novel, still poorly known Trichovirus in the family Betaflexiviridae. It is most closely related to Apple chlorotic leaf spot virus (ACLSV) (2,4) and infects stone fruit trees of the Prunus genus. Its presence has so far been detected in apricot, plum, Japanese plum, and peach trees in Italy, Spain, France, Hungary, Turkey, Jordan, and Australia (1,2,4). During the summers of 2008 and 2010, leaf samples of old Czech local plum cultivars were obtained from the Holovousy collection and assessed for the presence of viruses belonging to the Capillovirus, Trichovirus, and Foveavirus genera using the polyvalent degenerate oligonucleotides (PDO) nested reverse transcription (RT)-PCR test (3). Following amplification from total RNAs extracts, the amplicons were cloned and several clones were sequenced for each plant sample. In plum (Prunus domestica) cv. Babce, a mixture of amplicons was observed and BlastN and BlastX analyses of the obtained sequences revealed the presence of ACLSV and APCLSV. The 310-bp APCLSV amplicon (GenBank Accession No. JN790294) showed highest identity (82.9% in nucleotide sequence and 97.1% in amino acid sequence) with the Sus2 isolate of APCLSV (4) and clustered with APCLSV isolates in a phylogenetic analysis. APCLSV infection was further confirmed with an APCLSV-specific RT-PCR assay (4), which yielded a product of the expected 205-bp size (GenBank Accession No. JN653070) with closest homology again to the Sus2 APCLSV isolate (83.4 and 94.3% nucleotide and amino acid identity, respectively). To our knowledge, this finding represents the first detection of APCLSV in domestic plums in the Czech Republic, extending our vision of APCLSV diversity and its geographic distribution. For unknown reasons, APCLSV has almost always been reported in mixed infection with ACLSV (1,2,4) and the situation in cv. Babce does not deviate from this trend. This has greatly hindered the analysis of the pathogenicity of APCLSV, a situation further complicated in the current case because the Babce cultivar was also infected by Plum pox virus. References: (1) M. Barone et al. Acta Hortic. 781:53, 2008. (2) T. Candresse et al. Virus and Virus-Like Diseases of Pome and Stone Fruit Trees. A. Hadidi et al., eds. The American Phytopathological Society, St. Paul, MN, 2011. (3) X. Foissac et al. Phytopathology 95:617, 2005. (4) D. Liberti et al. Phytopathology 95:420, 2005.


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