Damage determination of western corn rootworm(Diabrotica virgifera virgiferaleconte) in soil disinfected, continuous corn

2007 ◽  
Vol 35 (2) ◽  
pp. 593-596 ◽  
Author(s):  
Sándor Keszthelyi ◽  
Tamás Szabó ◽  
Pál Kurucsai ◽  
Miklós Nádasy ◽  
Zsolt Marczali
Keyword(s):  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Continuous Corn

Incidence of Western Corn Rootworm Beetles (Coleoptera: Chrysomelidae) on Corn in Virginia from 1987 to 1992

1993 ◽  
Vol 28 (1) ◽  
pp. 136-141 ◽  
Author(s):  
Roger R. Youngman ◽  
Eric R. Day
Keyword(s):  
Crop Rotation ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
The State ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Continuous Corn ◽  
Corn Field ◽  
Survey Results ◽  
Diabrotica Virgifera Virgifera Leconte

The discovery of western corn rootworm beetles, Diabrotica virgifera virgifera LeConte, in a southwest Virginia corn field in 1985 prompted annual surveys of corn fields in an average of 28 counties across the state from 1987 to 1992. All counties included in the annual surveys were representative of the major corn-growing regions of Virginia. Survey results indicated that western corn rootworm beetles spread rapidly throughout most of the western and central continuous corn-growing regions of the state. In the eastern and southeastern corn-growing regions of the state, where crop rotation is widely practiced, detections of western corn rootworm beetles were less common and typically involved only one to two counties per year from 1987 to 1992.

scholarly journals Recent data to the distribution of western corn rootworm (Diabrotica virgifera virgifera LeConte) in Hungary

2000 ◽  
Vol 34 (4) ◽  
pp. 387-392 ◽  
Author(s):  
G. Ripka ◽  
G. Princzinger ◽  
I. Zsellér Hatala ◽  
László Vasas ◽  
B. Tóth ◽  
...  
Keyword(s):  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Diabrotica Virgifera Virgifera Leconte

scholarly journals Detection of alternative splicing in Diabrotica virgifera virgifera LeConte, in association with Bt resistance using RNA-seq and PacBio Iso-Seq

2020 ◽  
Author(s):  
Zixiao Zhao ◽  
Christine G. Elsik ◽  
Bruce E. Hibbard ◽  
Kent S. Shelby
Keyword(s):  
Alternative Splicing ◽  
Matrix Protein ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Rna Seq ◽  
Bt Resistance ◽  
Bt Toxins ◽  
Alternatively Spliced

AbstractBackgroundAlternative splicing is one of the major mechanisms that increases transcriptome diversity in eukaryotes, including insect species that have gained resistance to pesticides and Bt toxins. In western corn rootworm (Diabrotica virgifera virgifera LeConte), neither alternative splicing nor its role in resistance to Bt toxins has been studied.ResultsTo investigate the mechanisms of Bt resistance we carried out single-molecule real-time (SMRT) transcript sequencing and Iso-seq analysis on resistant, eCry3.1Ab-selected and susceptible, unselected, western corn rootworm neonate midguts which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. We present transcriptome-wide alternative splicing patterns of western corn rootworm midgut in response to feeding on eCry3.1Ab-expressing corn using a comprehensive approach that combines both RNA-seq and SMRT transcript sequencing techniques. We found that 67.73% of multi-exon genes are alternatively spliced, which is consistent with the high transposable element content of the genome. One of the alternative splicing events we identified was a novel peritrophic matrix protein with two alternative splicing isoforms. Analysis of differential exon usage between resistant and susceptible colonies showed that in eCry3.1Ab-resistant western corn rootworm, expression of one isoform was significantly higher than in the susceptible colony, while no significant differences between colonies were observed with the other isoform.ConclusionOur results provide the first survey of alternative splicing in western corn rootworm and suggest that the observed alternatively spliced isoforms of peritrophic matrix protein may be associated with eCry3.1Ab resistance in western corn rootworm.

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