Alimentary induced fatty liver and adjuvant therapy with effective natural bioactive molecules

Changes of redox-homeostasis generate cytokines, and free radicals influence many intracellular signaling pathways in different liver diseases. Liophylised table beet and carrot powder (GPS Powder Kft. 1361/004/2003BFÁÉÉÁ) containing bioactive components such as betaine, betanins, betaxanthins, flavonoids, polyphenols, glutamine, beta carotene, vitamins and folic acid may produce changes various cellular pathways. Aim: The aim of this study was to determine the protecting effects of bioactive agents of the liophylised table beet and carrot powder on fatty liver in a “short term” experiment. Method: Male Wistar rats were fed with chow with or without high fat (2% cholesterol, 0.5% cholic acid, 20% sunflower oil) and treated with 0.1 or 1 g/bwkg/day natural product for ten days parallel with the feedings. Cyclooxygenase-2, inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were determined using molecular biologic methods. Free radicals, H-donating activity, reducing power and free SH-group concentrations were determined by luminometry and spectrophotometry. Mobilized methyl groups were assayed by over pressure liquid chromatography method in liver homogenates. Results: It was found that the higher dose of the natural product better decreased the induced free radical reactions, cyclooxygenase-2, inducible nitric oxide synthase and tumor necrosis factor-α mRNA-levels both in normal and fatty liver tissues. Although treatments failed to exert significant changes in all global antioxidant parameters, mobilized methyl group concentrations were higher after treatments in fatty liver. Favorable tendencies were also noted in the redox-homeostasis of the fatty liver after treatment. Conclusions: As expected, lyophylised table beet and carrot proved to be a “functional food” in rats with alimentary fat induced fatty liver. It cannot be ruled out that this beneficial effect may have clinical relevance. Orv. Hetil., 2011, 152, 1035–1042.

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Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2021.110378 ◽

2022 ◽

pp. 110378

Author(s):

Shingo Ishikawa ◽

Masataka Miyazawa ◽

Chiho Tanaka ◽

Ryoma Uesawa ◽

Juri Nishizawa ◽

...

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis Factor ◽

Interferon Gamma ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Interferon Β ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide

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Serum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-α levels in patients with ischemic heart disease

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2008.213 ◽

2008 ◽

Vol 46 (8) ◽

Cited By ~ 10

Author(s):

Vidosava B. Djordjevic ◽

Ivana Stojanovic ◽

Vladan Cosic ◽

Lilika Zvezdanovic ◽

Marina Deljanin-Ilic ◽

...

Keyword(s):

Nitric Oxide ◽

Heart Disease ◽

Tumor Necrosis Factor ◽

Ischemic Heart Disease ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide

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β-Amyloid Stimulation of Inducible Nitric-oxide Synthase in Astrocytes Is Interleukin-1β- and Tumor Necrosis Factor-α (TNFα)-dependent, and Involves a TNFα Receptor-associated Factor- and NFκB-inducing Kinase-dependent Signaling Mechanism

Journal of Biological Chemistry ◽

10.1074/jbc.275.11.7918 ◽

2000 ◽

Vol 275 (11) ◽

pp. 7918-7924 ◽

Cited By ~ 210

Author(s):

Keith T. Akama ◽

Linda J. Van Eldik

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Signaling Mechanism ◽

Β Amyloid ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide ◽

Stimulation Of

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S-allylcysteine reduces the MPTP-induced striatal cell damage via inhibition of pro-inflammatory cytokine tumor necrosis factor-α and inducible nitric oxide synthase expressions in mice

Phytomedicine ◽

10.1016/j.phymed.2010.04.004 ◽

2010 ◽

Vol 18 (1) ◽

pp. 65-73 ◽

Cited By ~ 16

Author(s):

Esperanza García ◽

Juana Villeda-Hernández ◽

José Pedraza-Chaverrí ◽

Perla D. Maldonado ◽

Abel Santamaría

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis ◽

Inflammatory Cytokine ◽

Tumor Necrosis Factor Α ◽

Cell Damage ◽

Cytokine Tumor Necrosis Factor ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide

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EFFECTS OF KETAMINE/XYLAZINE ON EXPRESSION OF TUMOR NECROSIS FACTOR-α, INDUCIBLE NITRIC OXIDE SYNTHASE, AND CYCLO-OXYGENASE-2 IN RAT GASTRIC MUCOSA DURING ENDOTOXEMIA

Shock ◽

10.1097/01.shk.0000065766.72937.cf ◽

2003 ◽

Vol 20 (1) ◽

pp. 63-69 ◽

Cited By ~ 38

Author(s):

Kenneth S. Helmer ◽

Yan Cui ◽

Lily Chang ◽

Ashvin Dewan ◽

David W. Mercer

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis Factor ◽

Gastric Mucosa ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Rat Gastric Mucosa ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide

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Marked induction of inducible nitric oxide synthase and tumor necrosis factor-α in rat CD40+ microglia by comparison to CD40− microglia

Journal of Neuroimmunology ◽

10.1016/j.jneuroim.2009.01.007 ◽

2009 ◽

Vol 208 (1-2) ◽

pp. 70-79 ◽

Cited By ~ 11

Author(s):

Kohichi Kawahara ◽

Akira Yoshida ◽

Kiyohiro Koga ◽

Sumika Yokoo ◽

Akihiko Kuniyasu ◽

...

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis Factor ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Factor Α ◽

Oxide Synthase ◽

Necrosis Factor ◽

Inducible Nitric Oxide

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Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0098 ◽

2013 ◽

Vol 16 (4) ◽

pp. 693-699 ◽

Cited By ~ 8

Author(s):

T. Janowski ◽

W. Barański ◽

K. Łukasik ◽

D. Skarżyński ◽

M. Rudowska ◽

...

Keyword(s):

Nitric Oxide ◽

Tumor Necrosis Factor ◽

Mrna Expression ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Factor Α ◽

Oxide Synthase ◽

Subclinical Endometritis ◽

Necrosis Factor ◽

Inducible Nitric Oxide

Abstract Information on the prevalence of subclinical endometritis and its mechanism in repeat breeding cows is very limited. The aims of this study were: a) to evaluate the incidence of this disorder with cytobrush cytology b) to analyze mRNA expression of tumor necrosis factor α (TNFα) and inducible nitric oxide synthase (iNOS) in endometrial biopsy samples collected from repeat breeding cows with and without subclinical endometritis. Two experiments were carried out. In experiment 1, 112 (12.4%) repeat breeding cows (inseminated at least 3 times and not pregnant) were selected out of 902 cows from 8 dairy herds. Cytobrush cytology was performed on these cows, using the threshold of 10% PMNs in uterine smears. The results showed that 45 out of the 112 cows (40.2%) were diagnosed as having subclinical endometritis. In experiment 2, uterine biopsy samples were taken from repeat breeding cows with subclinical endometritis (n = 10) and without this disorder (n = 10). Using reverse transcription- PCR, the mRNA expression of TNFα and iNOS was determined. A statistically significant increase in expression of both substances was measured in the group of cows with subclinical endometritis (p < 0.05). These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFα and iNOS pathways in the regulation of this pathological condition.

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