Electrochemical-microscopy analysis of bio-functionalized diamond surfaces
AbstractDNA sensors fabricated on single-crystalline B-doped diamond (SBDD) electrodes were characterized by scanning electrochemical microscopy (SECM). These experiments show that circular arranged DNA sensors of typically 2 mm diameter show some spatial variations in electrochemical response. The variation of the diamond electrode with respect to DNA bonding is electrochemically characterized and discussed in the context of ion attraction/repulsion by the negatively charged backbones of DNA and the negatively charged diamond surface. Our results show that repulsive forces affect the mediator propagation of Fe(CN)64- in the close vicinity of the DNA layer which can be used to investigate DNA density variations on sensor areas.