Hyaluronic Acid-gelatin Fibrous Scaffold Produced by Electrospinning of Their Aqueous Solution for Tissue Engineering Applications

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

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Synthesis and characterization of polypyrrole-hyaluronic acid composite biomaterials for tissue engineering applications

Journal of Biomedical Materials Research ◽

10.1002/(sici)1097-4636(20000615)50:4<574::aid-jbm13>3.0.co;2-i ◽

2000 ◽

Vol 50 (4) ◽

pp. 574-584 ◽

Cited By ~ 266

Author(s):

Joel H. Collier ◽

James P. Camp ◽

Terry W. Hudson ◽

Christine E. Schmidt

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Synthesis And Characterization ◽

Engineering Applications ◽

Composite Biomaterials

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Hierarchical starch-based fibrous scaffold for bone tissue engineering applications

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.132 ◽

2009 ◽

Vol 3 (1) ◽

pp. 37-42 ◽

Cited By ~ 141

Author(s):

Albino Martins ◽

Sangwon Chung ◽

Adriano J. Pedro ◽

Rui A. Sousa ◽

Alexandra P. Marques ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Engineering Applications ◽

Fibrous Scaffold

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Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering

Polymers ◽

10.3390/polym11111772 ◽

2019 ◽

Vol 11 (11) ◽

pp. 1772 ◽

Cited By ~ 4

Author(s):

Beata Niemczyk-Soczynska ◽

Arkadiusz Gradys ◽

Dorota Kolbuk ◽

Anna Krzton-Maziopa ◽

Pawel Sajkiewicz

Keyword(s):

Aqueous Solution ◽

Tissue Engineering ◽

Hydrophobic Interactions ◽

Research Work ◽

Mechanical Analysis ◽

Biological Properties ◽

Ambient Conditions ◽

Scanning Calorimetry ◽

Engineering Applications ◽

Methoxy Groups

Thermosensitive, physically crosslinked injectable hydrogels are in the area of interests of various scientific fields. One of the representatives of this materials group is an aqueous solution of methylcellulose. At ambient conditions, methylcellulose (MC) is a sol while on heating up to 37 °C, MC undergoes physical crosslinking and transforms into a gel. Injectability at room temperature, and crosslinkability during subsequent heating to physiological temperature raises hopes, especially for tissue engineering applications. This research work aimed at studying crosslinking kinetics, thermal, viscoelastic, and biological properties of MC aqueous solution in a broad range of MC concentrations. It was evidenced by Differential Scanning Calorimetry (DSC) that crosslinking of MC is a reversible two-stage process, manifested by the appearance of two endothermic effects, related to the destruction of water cages around methoxy groups, followed by crosslinking via the formation of hydrophobic interactions between methoxy groups in the polymeric chains. The DSC results also allowed the determination of MC crosslinking kinetics. Complementary measurements of MC crosslinking kinetics performed by dynamic mechanical analysis (DMA) provided information on the final storage modulus, which was important from the perspective of tissue engineering applications. Cytotoxicity tests were performed using mouse fibroblasts and showed that MC at low concentration did not cause cytotoxicity. All these efforts allowed to assess MC hydrogel relevance for tissue engineering applications.

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