Synthesis of Cross-Linking Chitosan-Hyaluronic Acid Based Hydrogels for Tissue Engineering Applications

2017 ◽  
pp. 671-675 ◽  
Author(s):  
Nghi Thi-Phuong Nguyen ◽  
Long Vuong-Hoang Nguyen ◽  
Nam Minh-Phuong Tran ◽  
Thi-Hiep Nguyen ◽  
Chan-Khon Huynh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cross Linking ◽  
Engineering Applications

scholarly journals Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 794 ◽  
Author(s):  
Su Jeong Lee ◽  
Ji Min Seok ◽  
Jun Hee Lee ◽  
Jaejong Lee ◽  
Wan Doo Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Sodium Alginate ◽  
Three Dimensional ◽  
Fibroblast Cell ◽  
Chemical Crosslinking ◽  
Fibroblast Cell Line ◽  
Engineering Applications ◽  
Encapsulated Cells ◽  
Crosslinking Agents

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

Cellular Cross-linking of Peptide Modified Hydrogels

2004 ◽  
Vol 127 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Jeanie L. Drury ◽  
Tanyarut Boontheekul ◽  
David J. Mooney
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Density ◽  
Tensile Tests ◽  
Cross Linking ◽  
Engineering Applications ◽  
C2c12 Myoblasts ◽  
Polymer Systems ◽  
Peptide Modification ◽  
Adhesive Interactions

Peptide modification of hydrogel-forming materials is being widely explored as a means to regulate the phenotype of cells immobilized within the gels. Alternatively, we hypothesized that the adhesive interactions between cells and peptides coupled to the gel-forming materials would also enhance the overall mechanical properties of the gels. To test this hypothesis, alginate polymers were modified with RGDSP-containing peptides and the resultant polymer was used to encapsulate C2C12 myoblasts. The mechanical properties of these gels were then assessed as a function of both peptide and cell density using compression and tensile tests. Overall, it was found that above a critical peptide and cell density, encapsulated myoblasts were able to provide additional mechanical integrity to hydrogels composed of peptide-modified alginate. This occurred presumably by means of cell-peptide cross-linking of the alginate polymers, in addition to the usual Ca++ cross-linking. These results are potentially applicable to other polymer systems and important for a range of tissue engineering applications.

Cross-linking methods of electrospun fibrinogen scaffolds for tissue engineering applications

2008 ◽  
Vol 3 (4) ◽  
pp. 045001 ◽  
Author(s):  
Scott A Sell ◽  
Michael P Francis ◽  
Koyal Garg ◽  
Michael J McClure ◽  
David G Simpson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

Chemoselective cross-linking of alginate with thiol-terminated peptides for tissue engineering applications

2012 ◽  
Vol 88 (4) ◽  
pp. 1239-1250 ◽  
Author(s):  
Silvia Bubenikova ◽  
Izabela-Cristina Stancu ◽  
Lucia Kalinovska ◽  
Etienne Schacht ◽  
Evi Lippens ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

Role of temperature on bio-printability of gelatin methacryloyl bioink in two-step cross-linking strategy for tissue engineering applications

2020 ◽  
Vol 16 (1) ◽  
pp. 015021
Author(s):  
Mohsen Janmaleki ◽  
Jun Liu ◽  
Milad Kamkar ◽  
Milad Azarmanesh ◽  
Uttandaraman Sundararaj ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

Scaffolds and Tissue Engineering Applications by 3D Bio-Printing Process

2021 ◽  
pp. 718-733
Author(s):  
Ranjit Barua ◽  
Sudipto Datta ◽  
Pallab Datta ◽  
Amit Roy Chowdhury
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Manufacturing Process ◽  
Cross Linking ◽  
Engineering Applications ◽  
Printing Process ◽  
Important Thing ◽  
Bone Scaffolds ◽  
Complex Design ◽  
Different Types

3D bio-printing is a revolutionary manufacturing process that is widely used in medical fields especially in preparing bone scaffolds and tissue engineering. With the help of new biocompatible material like polymers, bio-gels, ceramics, this technology has created a new site in advanced tissue engineering and scaffolds manufacturing area. Another important thing is that, with the use of CAD file software, any complex design can be prepared (i.e., this technology does not have any limited sites). But here it is very much essential to study and analyze machine printability characteristics, cross-linking time and biocompatibility of printing objects as well as bio-ink. However, mechanical properties like shear thinning, mechanical elasticity are also required. In this chapter, different types of scaffold-preparing methods and the bio-printing process are discussed, which are used in scaffold and tissue engineering.

scholarly journals 3D Printing of Cytocompatible Water-Based Light-Cured Polyurethane with Hyaluronic Acid for Cartilage Tissue Engineering Applications

Materials ◽  
2017 ◽  
Vol 10 (2) ◽  
pp. 136 ◽  
Author(s):  
Ming-You Shie ◽  
Wen-Ching Chang ◽  
Li-Ju Wei ◽  
Yu-Hsin Huang ◽  
Chien-Han Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
3D Printing ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Engineering Applications ◽  
Water Based

Dual-Syringe Reactive Electrospinning of Cross-Linked Hyaluronic Acid Hydrogel Nanofibers for Tissue Engineering Applications

2006 ◽  
Vol 6 (10) ◽  
pp. 811-817 ◽  
Author(s):  
Yuan Ji ◽  
Kaustabh Ghosh ◽  
Bingquan Li ◽  
Jonathan C. Sokolov ◽  
Richard A. F. Clark ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Engineering Applications ◽  
Hyaluronic Acid Hydrogel
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