Image Forces on 3D Dislocation Structures in Crystals of Finite Volume

1998 ◽  
Vol 538 ◽  
Author(s):  
A. El-Azab

AbstractThe present work aims at studying the image stress and image Peach-Koehler force fields for three-dimensional dislocation configurations in a single crystal of finite volume. It is shown that the image stress field is significant within the entire crystal volume, and that the image Peach-Koehler force can be of the same order of magnitude as the direct interaction force calculated from the infinite domain solution. The results demonstrate that image stress gives rise to long-range interaction forces that are important in meso-scale dynamics of dislocation structures.

2021 ◽  
Vol 4 (3) ◽  
pp. 49
Author(s):  
Tomas Zelenka ◽  
Charalampos Spilianakis

The functional implications of the three-dimensional genome organization are becoming increasingly recognized. The Hi-C and HiChIP research approaches belong among the most popular choices for probing long-range chromatin interactions. A few methodical protocols have been published so far, yet their reproducibility and efficiency may vary. Most importantly, the high frequency of the dangling ends may dramatically affect the number of usable reads mapped to valid interaction pairs. Additionally, more obstacles arise from the chromatin compactness of certain investigated cell types, such as primary T cells, which due to their small and compact nuclei, impede limitations for their use in various genomic approaches. Here we systematically optimized all the major steps of the HiChIP protocol in T cells. As a result, we reduced the number of dangling ends to nearly zero and increased the proportion of long-range interaction pairs. Moreover, using three different mouse genotypes and multiple biological replicates, we demonstrated the high reproducibility of the optimized protocol. Although our primary goal was to optimize HiChIP, we also successfully applied the optimized steps to Hi-C, given their significant protocol overlap. Overall, we describe the rationale behind every optimization step, followed by a detailed protocol for both HiChIP and Hi-C experiments.


2000 ◽  
Vol 40 (supplement) ◽  
pp. S173
Author(s):  
Y. Sowa ◽  
T. Aoki ◽  
K. Kitamura ◽  
E. Muto ◽  
T. Yanagida

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Diyan Li ◽  
Chunyou Ning ◽  
Jiaman Zhang ◽  
Yujie Wang ◽  
Qianzi Tang ◽  
...  

AbstractFolliculogenesis is a complex biological process involving a central oocyte and its surrounding somatic cells. Three-dimensional chromatin architecture is an important transcription regulator; however, little is known about its dynamics and role in transcriptional regulation of granulosa cells during chicken folliculogenesis. We investigate the transcriptomic dynamics of chicken granulosa cells over ten follicular stages and assess the chromatin architecture dynamics and how it influences gene expression in granulosa cells at three key stages: the prehierarchical small white follicles, the first largest preovulatory follicles, and the postovulatory follicles. Our results demonstrate the consistency between the global reprogramming of chromatin architecture and the transcriptomic divergence during folliculogenesis, providing ample evidence for compartmentalization rearrangement, variable organization of topologically associating domains, and rewiring of the long-range interaction between promoter and enhancers. These results provide key insights into avian reproductive biology and provide a foundational dataset for the future in-depth functional characterization of granulosa cells.


Polymer ◽  
2016 ◽  
Vol 102 ◽  
pp. 363-371 ◽  
Author(s):  
Benedikt R. Neugirg ◽  
Nicolas Helfricht ◽  
Steffen Czich ◽  
Hans-Werner Schmidt ◽  
Georg Papastavrou ◽  
...  

1992 ◽  
Vol 114 (1) ◽  
pp. 48-54 ◽  
Author(s):  
S. Mukherjee ◽  
K. J. Waldron

Manipulation with three fingers in frictional contact is known to be an underspecified problem. The minimum norm solution, also referred to as the equilibrating solution field, can be modified by superimposing a linear combination of the resulting null solutions, called the interaction force field. The modification proposed here, applicable to the three-dimensional problem, minimizes the maximum value of the friction angle at the points of contract. A grasp of maximum possible stability under a set of contact and loading conditions is hence achieved.


1964 ◽  
Vol 13 (2) ◽  
pp. 127-128 ◽  
Author(s):  
J.A. Barker ◽  
P.J. Leonard

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