Aminopropyl Embedded Silica Films as Potent Substrates in DNA Microarray Applications

AbstractSol-gel derived silane hybrid films composing of (3-aminopropyl)trimethoxysilane–tetramethoxysilane (APTMS-TMOS) have been shown to have properties that make the films suitable for DNA microarray applications. The ability of the films to bind DNA was studied using aminated 25-mer oligonucleotide DNA and 1, 4-phenylenediisothiocyanate linker. The binding of DNA onto the films was shown to depend on films' composition i.e. the binding rate decreased with the decrease of APTMS content in the film. The arrayed primer extension (APEX) analysis showed reversed tendency – fluorescence signals of bound probes increased with the decrease of APTMS content in the film. The background noise was low and the fluorescent probes were clearly outlined. The density of functional amino groups on the films was estimated by fluorescence spectroscopy.

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Study on the Dielectric Properties of Hybrid and Porous Polyimide-Silica Films

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.47-50.973 ◽

2008 ◽

Vol 47-50 ◽

pp. 973-976 ◽

Cited By ~ 1

Author(s):

Yi He Zhang ◽

Qing Song Su ◽

Li Yu ◽

Hong Zheng ◽

Hai Tao Huang ◽

...

Keyword(s):

Dielectric Constant ◽

Sol Gel ◽

Silica Particles ◽

Low Dielectric Constant ◽

Hybrid Films ◽

Porous Films ◽

Silica Films ◽

Low Dielectric ◽

Dimethyl Acetamide ◽

Sol Gel Process

A sol-gel process was used to prepare polyimide-silica hybrid films from the polyimide precursors and TEOS in N,N- dimethyl acetamide, then the hybrid film was treated with hydrofluoric acid to remove the dispersed silica particles, leaving pores with diameters between 80nm to 1µm, depending on the size of silica particles. The structure and dielectric constant of the hybrid and porous films were characterized by FTIR,SEM. The porous films displayed relatively low dielectric constant compared to the hybrid polyimide-silica films.

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Single-Layer Silica Anti-Reflective Films Modified with Polytetramethylene Glycol

Materials Science Forum ◽

10.4028/www.scientific.net/msf.663-665.969 ◽

2010 ◽

Vol 663-665 ◽

pp. 969-973

Author(s):

Hong Tian ◽

Dong Jiang Yang ◽

Yao Xu

Keyword(s):

Colloidal Silica ◽

Sol Gel ◽

Single Layer ◽

Damage Threshold ◽

Silica Matrix ◽

Hybrid Films ◽

Sio2 Coating ◽

Silica Films ◽

Sol Gel Process ◽

Pure Sio2

Single-layer silica films modified with polytetramethylene glycol (PTMEG) were prepared via sol-gel process in the presence of NH4OH as catalyst. Compared with the pure SiO2 coating, the hybrid films possessed higher lased-induced damage threshold (LDT). The characterization results from AFM and TEM indicated that the introduction of PTMEG molecules changes the structure of the colloidal silica matrix, which directly resulted in the improvement of the transparency and LDT.

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DNA–DNA interaction on dendron-functionalized sol–gel silica films followed with surface plasmon fluorescence spectroscopy

Journal of Colloid and Interface Science ◽

10.1016/j.jcis.2007.01.041 ◽

2007 ◽

Vol 308 (2) ◽

pp. 325-331 ◽

Cited By ~ 11

Author(s):

Sung Hong Kwon ◽

Bong Jin Hong ◽

Hye Young Park ◽

Wolfgang Knoll ◽

Joon Won Park

Keyword(s):

Fluorescence Spectroscopy ◽

Surface Plasmon ◽

Sol Gel ◽

Dna Interaction ◽

Silica Films

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Preparation of Silica Hybrid Films on PMMA by Sol-Gel and Solvothermal Methods

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.785-786.877 ◽

2013 ◽

Vol 785-786 ◽

pp. 877-880

Author(s):

Xiao Gang Qin ◽

Sheng Sheng Yang ◽

Liang Shi ◽

An Xing ◽

Yuan Gao

Keyword(s):

Solvothermal Method ◽

Sol Gel ◽

Optical Transmittance ◽

Hybrid Films ◽

Silica Films ◽

Force Microscopy ◽

Uniform Particles ◽

Atomic Force ◽

Solvothermal Methods ◽

Silica Hybrid

Using solvothermal method to modify the surface of polymethyl methacrylate (PMMA), the silica films were prepared on it via sol solution and solvothermal treatment. Silica-based hybrid sol solutions were synthesized from tetraethoxysilane (TEOS) and methyltrimethoxysilane (MTMS). The obtained silica-based hybrid films consisted of the uniform particles around 40 nm in diameter observed by atomic force microscopy (AFM). The optical property was evaluated by the UV-Vis absorption spectra. The silica hybrid films prepared through this process showed good optical transmittance.

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Synthesis and Intermolecularly Mediated Assembly of Organic Pigment in Hybrid Coating Films

MRS Proceedings ◽

10.1557/proc-519-103 ◽

1998 ◽

Vol 519 ◽

Author(s):

Y. Yan ◽

Z. Duan ◽

D.-G. Chen ◽

S. Ray Chaudhuri

Keyword(s):

Sol Gel ◽

Hybrid Coating ◽

Hypsochromic Shift ◽

Hybrid Films ◽

Coating Films ◽

Organic Pigment ◽

Inorganic Hybrid ◽

Matrix Interactions ◽

Sol Gel Process

AbstractThe insoluble, strongly hydrogen bonded organic pigment of 3,6-bis-(4-chlorphenyl)-l,4- diketopyrrolo [3,4-c] pyrrole was transiently blocked by adding carbamate groups, and consequently incorporated into organic-inorganic hybrid matrices by a sol-gel process. The homo- (pigment-pigment) and hetero-intermolecular (pigment-matrix) interactions were found to control both the assembly and dispersion of pigment molecules in the hybrid coating films. A weaker interaction between matrices and pigment molecules results in aggregation of the carbamate pigment in the methyl-silicate films. A stronger interaction forms a homogenous dispersion and coloration of the phenyl-silicate films. The as-prepared methyl- and phenylsilicate films doped with the organic pigment were distinguished by a morphology change and a blue (hypsochromic) shift in absorption from 550 to 460 nm. Thermal treatment can remove the carbamate groups and in-situ form the organic pigment in the hybrid films.

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The preparation of poly(methylsilsesquioxane) network-polyimide hybrid materials by the sol-gel process and their properties

High Performance Polymers ◽

10.1088/0954-0083/6/1/005 ◽

1994 ◽

Vol 6 (1) ◽

pp. 43-52 ◽

Cited By ~ 24

Author(s):

Yoshitake Iyoku ◽

Masa-aki Kakimoto ◽

Yoshio Imai

Keyword(s):

Hybrid Materials ◽

Sol Gel ◽

Tensile Modulus ◽

Hybrid Films ◽

Elongation At Break ◽

Dimethyl Acetamide ◽

Flexible Films ◽

Sol Gel Process ◽

Heating Up ◽

Silicone Content

Poly(methylsilsesquixoane) network (silicone)-polyimide hybrid materials were successfully prepared by the sol-gel reaction of methyltriethoxysilane (MTES). The ethoxysilyl group in MTES was hydrolyzed and polycondensed in the solution of the polyamic acid, derived from pyromellitic dianhydride and bis(4-aminophenyl)ether, in N,N-dimethyl-acetamide (DMAc). The hybrid films were obtained by casting the reaction mixture, followed by heating up to 300°C. The hybrid materials containing 0-60wt% of silicone afforded flexible films. The films containing less than 7 wt% silicone were yellow and transparent, whereas the films with higher silicone content were yellow and opaque. Silicone particles with a diameter of around 1-10 μm were observed in the fracture surface of the hybrid films by scanning electron microscopy. Although the tensile strength and tensile modulus of the films obtained decreased with increasing silicone content. the value of the elongation at break remained at 60% up to 30% silicone content.

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Nanostructured Doped Silica Films Application as Sensitive Layers for Chemical Sensors

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.684.7 ◽

2013 ◽

Vol 684 ◽

pp. 7-11

Author(s):

Sergey Krutovertsev ◽

Alla Tarasova ◽

Olga Ivanova ◽

Larisa Krutovertseva

Keyword(s):

Initial Conditions ◽

Sol Gel ◽

Silica Films ◽

Adsorption Activity ◽

Developed Surface ◽

Sol Gel Process ◽

Sensitive Characteristics ◽

The Right ◽

Kinetics Of ◽

Matrix Factors

The sensor behavior of nanostructured doped silica films produced by sol-gel way were examined. Hygroscopic substances and polyoxometalates were used as additives to make more significant sensitive characteristics of initial matrix. Factors that have effect on sol preparation and films forming were investigated. Adsorption activity of the sensitive films was studied and it was shown that the films had a highly developed surface with nano-size pores. Change of initial conditions of sol-gel process gives opportunity to influence on kinetics of gel formation and consequently, on structure and properties of final materials. The study showed that the conditions of the environment affected the sensors characteristics markedly, which can be improved by choosing of the right procedure of forming and treatment. Influence of type and additive substances quantity into doped films was discussed in the paper

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