scholarly journals Taxonomy of alkalophilic Bacillus strains by analysing the nucleotide sequence of the 16S-RNA gene

2012 ◽  
Vol 28 (1) ◽  
Author(s):  
Tang Thi Chinh
Keyword(s):  
Nucleotide Sequence ◽  
16S Rna ◽  
Alkalophilic Bacillus ◽  
Bacillus Strains ◽  
16S Rna Gene

The number of ribosomes on simian virus 40 late 16S mRNA is determined in part by the nucleotide sequence of its leader

1984 ◽  
Vol 4 (4) ◽  
pp. 813-816
Author(s):  
A Barkan ◽  
J E Mertz
Keyword(s):  
Nucleotide Sequence ◽  
Direct Evidence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
The Body ◽  
Size Distributions ◽  
Wild Type ◽  
Leader Protein ◽  
16S Rna

The size distributions of polyribosomes containing each of three simian virus 40 late 16S mRNA species that differ in nucleotide sequence only within their leaders were determined. The two 16S RNA species with shorter leaders were incorporated into polysomes that were both larger (on average) and narrower in size distribution than was the predominant wild-type 16S RNA. Therefore, the nucleotide sequence of the leader can influence the number of ribosomes present on the body of an mRNA molecule. We propose a model in which the excision from leaders of sizeable translatable regions permits more frequent utilization of internally located translation initiation signals, thereby enabling genes encoded within the bodies of polygenic mRNAs to be translated at higher rates. In addition, the data provide the first direct evidence that VP1 can, indeed, be synthesized in vivo from the species of 16S mRNA that also encodes the 61-amino acid leader protein.

scholarly journals Nucleotide Sequence of the Xylanase A Gene of Alkalophilic Bacillus sp. Strain C-125

1987 ◽  
Vol 51 (3) ◽  
pp. 953-955
Author(s):  
Tetsuo Hamamoto ◽  
Hiroshi Honda ◽  
Toshiaki Kudo ◽  
Koki Horikoshi
Keyword(s):  
Nucleotide Sequence ◽  
Bacillus Sp ◽  
Alkalophilic Bacillus

Diversité des rhizobia associés au genre Hippocrepis en Algérie

Botany ◽  
2021 ◽  
Author(s):  
Samir Djouadi ◽  
Amina Bouherama ◽  
Fatiha AID ◽  
Saïd Amrani
Keyword(s):  
Atmospheric Nitrogen ◽  
Nitrogen Fixing ◽  
The North ◽  
16S Rna ◽  
Rhizobial Symbiosis ◽  
North And South ◽  
16S Rna Gene

La présente étude porte sur la nature des rhizobia associés aux légumineuses du genre Hippocrepis L. qui compte une dizaine d’espèces en Algérie et pour laquelle très peu de travaux ont été jusqu’ici consacrés. Les résultats de nos investigations qui ont portés sur 26 plants représentant 8 espèces de ce genre et provenant de 17 localités du nord et du sud du pays révèlent que tous les plants étaient nodulés et fixateurs d’azote, ce qui indique que les 8 espèces identifiées sont capables d’établir une symbiose efficiente avec les rhizobia présents dans les sols qui les supportent. Le séquençage du gène de l’ARN 16S des 26 souches de rhizobia isolées et authentifiées par des tests de nodulation au laboratoire, permet de les assigner à 16 espèces de rhizobia réparties sur 5 genres : Ensifer, Mesorhizobium, Bradyrhizobium, Rhizobium et Neorhizobium avec une prédominance des représentants des genres Ensifer et Mesorhizobium. Les résultats de cette étude qui est la première dédiée spécifiquement au genre Hippocrepis indiquent que la symbiose à rhizobia et la capacité d’utiliser, par ce biais, l’azote atmosphérique sont communes chez les représentants de ce genre en Algérie, ils révèlent aussi la grande diversité des rhizobia qui sont associés à ce genre. Abstract: The present study focuses on the nature of the rhizobia associated with legumes of the genus Hippocrepis L. which counts about ten species in Algeria and for which very little work has been performed so far. The results of our investigations, which focused on 26 plants representing 8 species of this genus and coming from 17 localities in the north and south of the country, show that all plants were nodulated and nitrogen-fixing, which indicates that the species identified are capable of establishing an efficient symbiosis with the rhizobia present in the soils that support them. The sequencing of the 16S RNA gene of the 26 strains of rhizobia isolated and authenticated by nodulation tests in the laboratory, allows their assignation to16 species of rhizobia spread over 5 genera: Ensifer, Mesorhizobium, Bradyrhizobium, Rhizobium and Neorhizobium with a predominance of representatives of the genera Ensifer and Mesorhizobium. The results of this study, which is the first dedicated to the genus Hippocrepis, show that the rhizobial symbiosis and its ability to use atmospheric nitrogen are common among representatives of the genus Hippocrepis in Algeria and reveal a great diversity of associated rhizobia

Changes in 16s RNA Gene Microbial Community Profiling by Concentration of Prokaryotic DNA

2015 ◽  
Vol 119 ◽  
pp. 239-242 ◽  
Author(s):  
Angela Glassing ◽  
Scot E. Dowd ◽  
Susan Galandiuk ◽  
Brian Davis ◽  
Jeffrey R. Jorden ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Community Profiling ◽  
Community Profiling ◽  
16S Rna ◽  
16S Rna Gene

scholarly journals Ultraviolet Irradiation of Skin Alters the Faecal Microbiome Independently of Vitamin D in Mice

Nutrients ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1069 ◽  
Author(s):  
Simon Ghaly ◽  
Nadeem Kaakoush ◽  
Frances Lloyd ◽  
Lavinia Gordon ◽  
Cynthia Forest ◽  
...  
Keyword(s):  
Vitamin D ◽  
High Throughput Sequencing ◽  
Dorsal Skin ◽  
Faecal Dna ◽  
Skin Exposure ◽  
16S Rna ◽  
Health And Disease ◽  
Faecal Microbiome ◽  
16S Rna Gene ◽  
Inflammatory Effect

Reduced sunlight exposure has been associated with an increased incidence of Crohn’s disease and ulcerative colitis. The effect of ultraviolet radiation (UVR) on the faecal microbiome and susceptibility to colitis has not been explored. C57Bl/6 female mice were fed three different vitamin D-containing diets for 24 days before half of the mice in each group were UV-irradiated (1 kJ/m2) for each of four days, followed by twice-weekly irradiation of shaved dorsal skin for 35 days. Faecal DNA was extracted and high-throughput sequencing of the 16S RNA gene performed. UV irradiation of skin was associated with a significant change in the beta-diversity of faeces compared to nonirradiated mice, independently of vitamin D. Specifically, members of phylum Firmicutes, including Coprococcus, were enriched, whereas members of phylum Bacteroidetes, such as Bacteroidales, were depleted. Expression of colonic CYP27B1 increased by four-fold and IL1β decreased by five-fold, suggesting a UVR-induced anti-inflammatory effect. UV-irradiated mice, however, were not protected against colitis induced by dextran sodium sulfate (DSS), although distinct faecal microbiome differences were documented post-DSS between UV-irradiated and nonirradiated mice. Thus, skin exposure to UVR alters the faecal microbiome, and further investigations to explore the implications of this in health and disease are warranted.

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