scholarly journals Screening of hybridoma cell lines secreting monoclonal antibodies specific for staphylococcal enterotoxin B (SEB) derived from Staphylococcus aureus

2016 ◽  
Vol 14 (1) ◽  
pp. 23-28
Author(s):  
Nghiêm Ngọc Minh ◽  
Nguyễn Thị Hoài Thu ◽  
Phạm Thùy Linh ◽  
Thân Đức Dương ◽  
Vũ Thị Thu Hằng ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibodies ◽  
B Lymphocytes ◽  
Food Poisoning ◽  
Gastrointestinal Diseases ◽  
Recombinant Antigen ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B ◽  
High Level

Staphylococcus aureus (S. aureus) produces 11 types of toxins and more than 20 different Staphylococcal enterotoxins (SEs), including SEA to SEE, SEG to SER and SEU. Among them, enterotoxin type B (Staphylococcal enterotoxin B - SEB) is quite heat stable and causes gastrointestinal diseases in food poisoning. The symptoms of SEB intoxication begin with the onset of sudden fever, about 40oC to 41oC, chills, headache, muscle aches and dry cough. Some patients feel shortness of breath and chest pain. Although SEB is not considered lethal, high level of exposure can lead to shock and death. Therefore, a nontoxic SEB recombinant antigen was produced to immunize mice to create B lymphocytes. Myeloma cells were fused with the B lymphocytes to generate  hybridoma lines. The screening of monoclonal antibodies for the SEB antigen was determined by ELISA and Western blot tests. This study demonstrates that an SEB recombinant antigen can immunize a response against SEB in BALB/c mice. The production of monoclonal antibodies will be used to make a rapid detection strip for SEB based on immunochromatography.

scholarly journals Staphylococcal enterotoxin B (SEB) toxin of Staphylococcus aureus and the detection methods

2018 ◽  
Vol 15 (2) ◽  
pp. 211-221
Author(s):  
Nguyễn Thị Hoài Thu ◽  
Nghiêm Ngọc Minh
Keyword(s):  
Staphylococcus Aureus ◽  
Proteolytic Enzymes ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Water Soluble ◽  
Detection Methods ◽  
Staphylococcal Enterotoxin B ◽  
Heat Stable ◽  
Enterotoxin B ◽  
Basic Features

Staphylococcal enterotoxins (SEs) secreted by Staphylococcus aureus is one of the principal causes of food poisoning. The SEs are superantigens; they are highly stable, resisting most proteolytic enzymes and thus keeping activity in the gastrointestinal tract after being ingestion. In particular, heat-stable enterotoxin is one of the most important property related to food safety. They are not degraded at 100°C for 30 minutes, even at 121oC for 28 minutes, the SEs retain biological activity. Heat resistance of SEs in foods is higher than in the culture medium. Staphylococcus aureus (S. aureus) produces more than 20 different types of enterotoxins, including SEA to SEE, SEG to SER and SEU. Among these, Staphylococcal enterotoxin B (SEB) is a powerful toxin, heat-stable, water-soluble and is a common cause of food poisoning. Moreover, SEB is one of the harmful or hazardous agents used as biological weapons in bioterrorism or biological warfare. Therefore, determining presence of SEB toxin in food is extremely important. In this review, we introduce the most basic features about S. aureus; about SEB toxin and conventional methods for SEB diagnosis, detection. Especially, we focus on rapid detection strip based on an immunochromatography; this technique is an highly sensitive, rapid, easy for use and storage.

Influences of σBandagron expression of staphylococcal enterotoxin B (seb) inStaphylococcus aureus

2004 ◽  
Vol 50 (5) ◽  
pp. 351-360 ◽  
Author(s):  
Katherine A Schmidt ◽  
Niles P Donegan ◽  
William A Kwan, Jr. ◽  
Ambrose Cheung
Keyword(s):  
Fluorescent Protein ◽  
Food Poisoning ◽  
Inverse Correlation ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Gfp Expression ◽  
Enterotoxin B ◽  
Specific Regulation ◽  
Green Fluorescent ◽  
High Level

In Staphylococcus aureus, enterotoxin B (SEB) is a superantigen that activates host interleukins and induces adverse responses, ranging from food poisoning to toxic shock. The alternate sigma factor, σB(SigmaB), and agr are two known regulators of S. aureus. Northern blots of strain COL, a sigB-positive strain, showed an inverse correlation between σBexpression and seb message. seb expression was also measured as a function of a seb promoter linked to green fluorescent protein (GFP) expression in RN6390, COL, and Newman. In sigB mutants of RN6390, SH1000, COL, and Newman, seb promoter activities, as measured by GFP expression, increased relative to the respective parental types but at differing levels, suggesting alternate strain-specific regulation. In agr mutants of RN6390 and Newman, seb promoter activities were intermediate between the high level seen for the sigB mutant and the low level in the sigB active strains. A sigB agr double mutant of RN6390 displayed lower GFP expression than the agr mutant. These results suggest that while σBand agr regulate seb expression in a divergent manner, other activator(s) of seb that depend on sigB expression may be present in S. aureus.Key words: staphylococcal enterotoxin B, σBregulation, agr regulation.

scholarly journals Acute Systemic Immune Activation following Conjunctival Exposure to Staphylococcal Enterotoxin B

2006 ◽  
Vol 74 (10) ◽  
pp. 6016-6019 ◽  
Author(s):  
Govindarajan Rajagopalan ◽  
Michele K. Smart ◽  
Robin Patel ◽  
Chella S. David
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Activation ◽  
Human Leukocyte Antigen Class ◽  
Human Leukocyte ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Leukocyte Antigen ◽  
Systemic Immune Activation ◽  
Enterotoxin B ◽  
First Time

ABSTRACT Conjunctival exposure to the Staphylococcus aureus superantigen staphylococcal enterotoxin B (SEB) may occur accidentally, as a result of bioterrorism, or during colonization or infection of the external eye. Using human leukocyte antigen class II transgenic mice, we show for the first time that conjunctival exposure to SEB can cause robust systemic immune activation.

scholarly journals Mechanisms Mediating Enhanced Neutralization Efficacy of Staphylococcal Enterotoxin B by Combinations of Monoclonal Antibodies

2015 ◽  
Vol 290 (11) ◽  
pp. 6715-6730 ◽  
Author(s):  
Kaushik Dutta ◽  
Avanish K. Varshney ◽  
Matthew C. Franklin ◽  
Michael Goger ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

scholarly journals Staphylococcus aureus Isolates Encode Variant Staphylococcal Enterotoxin B Proteins That Are Diverse in Superantigenicity and Lethality

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41157 ◽  
Author(s):  
Petra L. Kohler ◽  
Seth D. Greenwood ◽  
Suba Nookala ◽  
Malak Kotb ◽  
David M. Kranz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

scholarly journals Effective Treatment of Staphylococcal Enterotoxin B Aerosol Intoxication in Rhesus Macaques by Using Two Parenterally Administered High-Affinity Monoclonal Antibodies

2019 ◽  
Vol 63 (5) ◽  
Author(s):  
Daniel Verreault ◽  
Jane Ennis ◽  
Kevin Whaley ◽  
Stephanie Z. Killeen ◽  
Hatice Karauzum ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nonhuman Primate ◽  
Rhesus Macaques ◽  
Clinical Signs ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Primate Model ◽  
Content Type ◽  
Enterotoxin B

ABSTRACTStaphylococcal enterotoxin B (SEB) is a protein exotoxin found on the cell surface ofStaphylococcus aureusthat is the source for multiple pathologies in humans. When purified and concentrated in aerosol form, SEB can cause an acute and often fatal intoxication and thus is considered a biological threat agent. There are currently no vaccines or treatments approved for human use. Studies with rodent models of SEB intoxication show that antibody therapy may be a promising treatment strategy; however, many have used antibodies only prophylactically or well before any clinical signs of intoxication are apparent. We assessed and compared the protective efficacies of two monoclonal antibodies, Ig121 and c19F1, when administered after aerosol exposure in a uniformly lethal nonhuman primate model of SEB intoxication. Rhesus macaques were challenged using small-particle aerosols of SEB and then were infused intravenously with a single dose of either Ig121 or c19F1 (10 mg/kg of body weight) at either 0.5, 2, or 4 h postexposure. Onset of clinical signs and hematological and cytokine response in untreated controls confirmed the acute onset and potency of the toxin used in the challenge. All animals administered either Ig121 or c19F1 survived SEB challenge, whereas the untreated controls succumbed to SEB intoxication 30 to 48 h postexposure. These results represent the successful therapeuticin vivoprotection by two investigational drugs against SEB in a severe nonhuman primate disease model and punctuate the therapeutic value of monoclonal antibodies when faced with treatment options for SEB-induced toxicity in a postexposure setting.

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