scholarly journals Shoot multiplication via elongated stem node culture under darkness: a novel method in micropropagation of Paphiopedilum villosum

2018 ◽  
Vol 16 (3) ◽  
pp. 491-499
Author(s):  
Nguyen Phuc Huy ◽  
Dang Thi Tinh ◽  
Vu Quoc Luan ◽  
Hoang Thanh Tung ◽  
Vu Thi Hien ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Regeneration Rate ◽  
Orchid Species ◽  
Stem Node ◽  
Multiplication Coefficient ◽  
Novel Method ◽  
Node Culture

Paphiopedilum villosum is a beautiful orchid species and has high value in trade; however, this is one of the most difficult to propagate orchids. So far, there has been very little publication on micropropagation. In this study, the effects of plant growth regulators on shoot regeneration from stem node culture of elongated P. villosum shoots in the darkness were investigated. Shoots (1.5 cm) were elongated and produced individual stem nodes under darkness condition for 3 months. Stem nodes were cultured on SH medium and supplemented with individually BA, KIN or TDZ to investigate shoot regeneration. The shoot multiplication rate was also recorded in this study. The highest stem node was observed in the dark with 5.25 cm in the height and the number of stem nodes were 3 stem nodes/shoot. The isolated stem node was cultured on SH medium supplemented with 30 g/L sucrose, 8 g/L agar, 1 g/L charcoal and different concentrations of BA, KIN, TDZ. The results observed after 3 months showed that the best shoot regeneration rate (85%) and highest shoot multiplication coefficient (6.6 shoots/stem node) was obtained when shoots derived from stem node were cultured on SH medium supplemented with 0.5 mg/L TDZ, 30 g/L sucrose, 8 g/L agar and 1 g/L charcoal. Those shoots obtained in the above treatments were subcultured on MS medium supplemented with 0.3 mg/L NAA for rooting and gave the highest number of roots (6.6 roots/shoot) after 1 month; and these plantlets were acclimatized in Taiwan sphagnum moss and transferred into greenhouse with the best survival rate (100%) after 3 months.

scholarly journals Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai’

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1253-1255 ◽  
Author(s):  
Aish Muhammad ◽  
Hamid Rashid ◽  
Iqbal Hussain ◽  
S.M. Saqlan Naqvi
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Height ◽  
Significant Difference ◽  
Rate Effects ◽  
Single Shoot ◽  
Number Of Shoots

The effect of benzylaminopurine (BAP) and kinetin alone and in combination with indole 3-acetic acid (IAA) on shoot proliferation of ‘Basrai’ (Musa spp., AAA group) was investigated. Shoot tips (4–6 mm) were excised from field-grown suckers to initiate the cultures. Concentrations of BAP and kinetin ranged from 0.0 to 8.0 mg·L−1 each on solid or in liquid MS medium. When liquid medium was used, cultures were agitated continuously on an orbital shaker moving at 40 rpm. Three subculture regimes were employed; after each subculture, the number of shoots regenerated from each explant was counted. The results showed that the multiplication rate was significantly (P ≤ 0.05) dependent upon cytokinin type, its concentration, and type of medium used. The maximum number of shoots regenerated from a single shoot tip was achieved in liquid MS medium containing 4.0 mg·L−1 BAP. There was no significant difference between liquid and solid medium when kinetin was used; however, kinetin at 4.0 mg·L−1 or above yielded significant results as compared with the control and lower kinetin concentrations. The results demonstrated that 4.0 mg·L−1 BAP with 1.0 mg·L−1 IAA in liquid medium was best for shoot multiplication and shoot height during micropropagation of ‘Basrai’.

scholarly journals The effect of BA and GA3 on the shoot multiplication of in vitro cultures of Polish wild roses

2011 ◽  
Vol 23 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Bożena Pawłowska
Keyword(s):  
Relative Humidity ◽  
Ornamental Plants ◽  
Shoot Multiplication ◽  
In Vitro Cultures ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Naturally Occurring ◽  
Dog Rose ◽  
The University

The effect of BA and GA3on the shoot multiplication ofin vitrocultures of Polish wild rosesThe experiment was conducted using five species of roses naturally occurring in Poland:Rosa agrestis(fieldbriar rose),R. canina(dog rose),R. dumalis(glaucous dog rose),R. rubiginosa(sweetbriar rose), andR. tomentosa(whitewooly rose), from thein vitrocollection of the Department of Ornamental Plants of the University of Agriculture in Kraków. We examined the effect of cytokinin BA (1-10 μM) added to an MS medium (Murashige and Skoog 1962) on auxiliary shoot multiplication. The second group of test media contained BA (1-5 μM) and gibberellin GA3(0.3-1.5 μM). The cultures were maintained at a phytotron temperature of 23/25°C (night/day), 80% relative humidity, with a 16-hour photoperiod and PPFD of 30 μmol m-2s-1, and cultured in five-week cycles. The highest multiplication rate was obtained forR. caninaandR. rubiginosa(4.1 shoots per one explant) andR. dumalis(2.9 shoots per one explant), when shoots were multiplied on an MS medium supplemented with 1 μM BA and 1.5 μM GA3. Multiplication was the weakest inRosa tomentosaindependent of the medium used.

scholarly journals Micropropagation ofOriganum acutidens(HAND.-MAZZ.) IETSWAART Using Stem Node Explants

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Mehmet Ugur Yildirim
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Landscape Management ◽  
Shoot Proliferation ◽  
Ornamental Plant ◽  
Peat Moss ◽  
Ms Medium ◽  
Regeneration Rate ◽  
Stem Node

Origanum acutidens(HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0.6, 1.2, 1.8, and 2.4 mg/L BAP with 0.2 mg/L NAA. Visible effects of culture media on shoot proliferation were recorded. Shoot regeneration rate was maximum on MS medium containing 1.80 mg/L BAP-0.2 mg/L NAA. The micropropagated shoots were rooted on MS medium containing 0.2 mg/L NAA. All microrooted plantlets survived during acclimatisation on peat moss. It was concluded thatO. acutidenscan be successfully micropropagated underin vitroconditions.

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals Determination of Apposite Plant Regeneration Protocol for Several Cucurbits through Direct and Indirect Organogenesis

1970 ◽  
pp. 4-13
Author(s):  
Mohammad Firoz Alam ◽  
Palash C. Mondol ◽  
Sushanta Kumar Roy ◽  
Muhammad Anisuzzaman ◽  
Saroar Parvez ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Casein Hydrolysate ◽  
Shoot Multiplication ◽  
Leaf Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Nodal Cutting ◽  
Dichlorophenoxy Acetic Acid

A competent and reproducible practice for the invitro shoot regeneration of Cucurbita maxima,C.pepo and Cucumissativus was developed from various explants through direct and indirect organogenesis.InC. maxima, between cotyledon and leaf segment, cotyledon was found to be most responsive for callus induction in MS medium augmented with 0.5 mg·L-1 2,4 dichlorophenoxy acetic acid (2,4-D) plus 100 mg·L-1 casein hydrolysate and 0.5 mg·L-1 2,4-D plus 15% coconut water and for leaf segment it was on MS medium containing 2.5 mg·L-1 2,4-D. Comparing the 2 explants it was found that leaf segment was most suitable for callus induction in C. maxima. For massive multiplication of C. pepomericlones shoot tip and nodal cutting were used. MS medium containing 3.0 mg·L-1 6-benzyl aminopurine plus 0.5 mg·L-1gibberellic acid (GA3) was found most effective for shoot regeneration and 1.0 mg·L-1 IBA was found most effective for rooting. In this trait cv. Bulum was more responsive than cv. Rumbo. On the other hand, to generate virus free plantlets of C. sativus, different concentrations of kinetin were used, and 1.5 mg·L-1 KIN shown the best performance for primary culture establishment. For shoot multiplication, 1.0 mg·L-1 BAP and 2.0 mg·L-1 BAP plus 0.5 mg·L-1 KIN containing medium shown best result. Subsequently, 2.0 mg·L-1 BAP plus 0.5 mg·L-1 KIN was best composition for root induction. Our report demonstrated comprehensive protocols and variability in explants, growth regulator response in shoot regeneration potential of in different cucurbit plants.

scholarly journals A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.)

2019 ◽  
Vol 1 (4) ◽  
pp. 261-269
Author(s):  
Thuy Linh Thi Nguyen ◽  
Ngoc Thi Pham ◽  
Thao Thi Ninh ◽  
Phuong Thao Thi Nguyen
Keyword(s):  
Climate Adaptation ◽  
Shoot Multiplication ◽  
Good Growth ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Rice Husks ◽  
Shoot Initiation ◽  
Primary Explants ◽  
Planting Materials

This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA. The highest shoot multiplication rate (9.57 times) was obtained on MS medium containing 1 mg L-1 BA and 0.25 mg L-1 a-NAA after 8 weeks of culture. 100% of the shoots produced roots with a mean of 10.10 roots per plant within 4 weeks on ½ MSM medium with 4 mg L-1 IBA. The survival rate of in vitro derived plantlets after a 6 to 7-week-period of rooting during acclimatization using a soil: coco peat: smoked rice husks (2:2:1, v/v/v) substrate was 100% and acclimatized plantlets showed good growth and development. This is the first report on a practical and efficient in vitro multiplication protocol for Japanese cherry in Vietnam, starting from shoot initiation to establishment of plants under greenhouse conditions.

scholarly journals A Novel Method for Rapid Micropropagation of Pineapple

HortScience ◽  
1995 ◽  
Vol 30 (1) ◽  
pp. 127-129 ◽  
Author(s):  
E. Kiss ◽  
J. Kiss ◽  
G. Gyulai ◽  
L.E. Heszky
Keyword(s):  
Growth Regulator ◽  
Shoot Elongation ◽  
Ananas Comosus ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration Rate ◽  
Novel Method ◽  
N6 Medium ◽  
Regenerated Plantlets

A novel micropropagation method for pineapple (Ananas comosus L.), based on shoot elongation induced in vitro, was demonstrated for two cultivars. Decapitated in vitro plantlets were used as explants. Shoot etiolation was induced by placing explants in a Murashige and Skoog (MS) medium containing NAA (10 μm) and incubating in darkness at 28C for 30 to 40 days. The mean number of the regenerated etiolated shoots per explant was 2.6 ± 0.29. The etiolated shoots were placed into N6 medium supplemented with kinetin or BA (25 or 20 μm, respectively). After 4 to 6 weeks, shoots regenerated along the nodes. The highest regeneration rate was 15 and 13 plantlets per node with 25 μm kinetin and 20 mm BA, respectively. Regenerated plantlets were rooted on a growth-regulator-free MS medium. Residual shoots of the initial explants could be recycled by rooting on a growth-regulator-free MS medium. This procedure enables the regeneration of several thousand plantlets per year. Chemical names used: naphthaleneacetic acid (NAA); benzyladenine (BA).

Indirect shoot regeneration of Iranian purple coneflower (Echinacea purpurea L.) from cotyledon and hypocotyl explants

2011 ◽  
Vol 59 (1) ◽  
pp. 65-72 ◽  
Author(s):  
A. Zebarjadi ◽  
J. Motamedi ◽  
A. Ismaili
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Shoot Formation ◽  
Ms Medium ◽  
Medicinal Species ◽  
Regeneration Rate ◽  
Hypocotyl Explants ◽  
Purple Coneflower ◽  
Significant Difference

In vitro plant regeneration was optimized for Iranian purple coneflower via organogenesis from callus cultures derived from cotyledon and hypocotyl tissues by placing them on MS medium supplemented with different concentrations and combinations of BAP and NAA. The experiment was laid out as a completely randomized design in a factorial arrangement with three replications. The results indicated that the mean callus induction was influenced by explant type, with a significant difference between cotyledon (77.81%) and hypocotyl (65.33%) explants at the 0.01 probability level. In relation with the regeneration rate, no significant differences were observed between the two types of explants. For both cotyledons and hypocotyls the optimum shoot regeneration frequency (31.5% and 32.5%, respectively) and number of shoots per explant (5.2 and 5.3, respectively) were achieved using medium supplemented with 0.4 mg l−1 BAP. Proliferated shoots were elongated in hormone-free MS medium and well-developed shoots were rooted on MS medium, both with and without the addition of 2 mg l−1 IBA. All the plantlets survived acclimatization, producing normal plants under controlled conditions. This study revealed that cotyledon and hypocotyl explants of E. purpurea have relatively good potential for callus induction and shoot formation. Furthermore, a beneficial method has been established for the micropropagation of this valuable medicinal species.

scholarly journals In vitro propagation and growth of ex vitro Chrysanthemum indicum L. in Da Lat - Lam Dong

2021 ◽  
Vol 19 (1) ◽  
pp. 175-184
Author(s):  
Phan Xuan Huyen ◽  
Truong Ngoc Thao Vy ◽  
Nguyen Thi Phuong Hoang ◽  
Nguyen Thi Thanh Hang ◽  
Dinh Van Khiem
Keyword(s):  
Plant Height ◽  
Shoot Regeneration ◽  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Ms Medium ◽  
Coconut Fiber ◽  
Regeneration Rate ◽  
Shoot Height ◽  
Chrysanthemum Indicum

Chrysanthemum indicum plant is a valuable and beneficial herb for human health. In this paper, we present the results of in vitro propagation of this plant in Da Lat. The results showed that MS medium supplemented with 25 g/l sucrose, 9 g/l agar, pH 5.8 was the best for shoot regeneration and growth (shoot height was 2.41 - 2.47 cm, 1 shoot/explant). MS media supplemented with BA (0.1, 0.5, 1, 1.5, 2 mg/l), Kinetin (0.1, 0.5, 1, 1.5, 2 mg/l ) and TDZ (0.1, 0.5, 1 mg/l) were unsuitable for shoot regeneration and growth of C. indicum. The regeneration and growth of shoots on the medium supplemented with 1 g/l of activated charcoal was better than (plant height of 3.45 cm) medium without activated charcoal (plant height of 2.46 cm). MS medium supplemented with 0, 0.1, 0.5, 1 mg/l IBA, 25 g/l sucrose, 9 g/l agar, pH 5,8 were suitable for in vitro root regeneration, rate of root formation was 100%. Coconut fiber powder was the best substrate to transfer the plantlets to the greenhouse, with survial rate of 100%, plantlets grew well. Nitrophoska irrigation was the best for the growth and development of C. indicum cultivated on coconut fiber powder under greenhouse conditions (plant height of 61.70 cm, 100.80 flowers/tree, flower diameter of 1.68 cm, fresh weight of 0.376 g/flower). The results also show that seedlings derived from tissue culture can be used to cultivate C. indicum and C. indicum grew well in the climate of Da Lat - Lam Dong and flowered all year round.

Sign in / Sign up

Export Citation Format

Share Document