CHARACTERIZATION OF SOME ALGERIANS AND FOREIGN LENTIL ACCESSIONS BY QUALITATIVE TRAITS

In the present study an attempt has been made to characterize lentil accessions based on qualitative traits. There were variations among 44 lentil accessions. Erect growth habit was observed in 24 % of the accessions where 8% were from Algeria. Conversely, prostrate growth habit was observed in 34% of the accessions. The majority of the remaining accessions (41.32%) were intermediate. Stem with anthocyanin pigmentation was showed in 44% of the accessions, whereas, 56% had no pigmentation (green stem). About half of the accessions had grey green leaves (53%) and 23% of accessions were light green. Among the characters, flower color showed the highest variation. White flowers were observed in 64% accessions and violet flowers were found in 36% accessions. Flowers, with violet stripes in the standard petal (SVE) were observed in 44% accessions and the majority (56%) lacked violet stripes. Yellow cotyledons were observed in 61% accessions, while the rest (39%) had red cotyledons. The majority of accessions (81%) were observed with brown testa while 14% were green and 5% had yellow testa. Absence of seed coat pattern was observed in 69% accessions. However, 8% accessions with spots, 5% with dots, 16% were marbled and the remaining 2% were complex. Flattened seed shape was observed in 60% of accessions. Conversely, globose shape was observed in 40% of the accessions, among of them 27% were from Algeria.

Influence of medium and growth regulators on callogenesis of quinoa (Chenopodium quinoa Willd.) and effect of hydrous stress induced by P.E.G 6000 on the callus

The induction and growth of quinoa’s callus depend on several factors, including the culture medium and the nature of the growth hormone and its dose. In effect, the best callogenesis rates were obtained with the media MS and B5 with respect to the media WHITE and KNOP the callogenesis is too low or zero. The best combination used was 0.2 BA + 2.4 D give well-developed callus. To obtain water-stress resistant cell lines, the effect of water stress induced by polyethylene glycol (P.E.G 6000) on the growth, osmotic potential and metabolic parameter of Chenopodium quinoa callus was studied. Applied water stress showed a reduction in the growth of stressed callus compared to the control. The presence of PEG in the culture medium caused a decrease in the content of fresh matter as well as the dry matter content compared to the control. Water stress also significantly affected the water parameters of calluses. The chlorophyll a, b and carotenoids content decreased, but this decrease is not too pronounced.

A comparative method for protein extraction and proteome analysis by two-dimensional gel electrophoresis from banana fruit

<p>Bananas and plantains are a major staple food and export product in many countries.<strong> </strong>Banana fruit tissues contain large amounts of secondary compounds, polysaccharides and other plant polymers which will interfere with protein extraction for gel-based proteomic analysis. Due to presence of very small amount of proteins (approximately 1%) in a large matrix of fruit tissues, it classified as ‘recalcitrant’. In this connection, assessment of different protein extraction protocols and extraction of high quality proteins from banana fruit tissues is crucial for successful gel-based proteome analysis. In this study, two different protein extraction protocols were validated to isolate proteins from banana (cv.Grand Naine) fruit peel and pulp tissues, and proteins were resolved by using SDS-PAGE. A comparative study showed that phenol based method is effective in extracting proteins over TCA-acetone method. Isolated proteins were further subjected to two-dimensional gel electrophoresis separation and stained with colloidal coomassie blue to visualize protein spots. On average 380 protein spots could be detected on coomassie stained two-dimensional gel and our study clearly demonstrated the differential protein accumulation during pre-climacteric and climacteric stages of banana fruit. <strong></strong></p>

Genetic variability among Fusarium oxysporum isolates from melon (Cucumis melo) in Qazvin province, Iran

<p><em>Fusarium</em> wilt, caused by <em>Fusarium oxysporum</em> f. sp. <em>melonis</em> (Fom), the soil-borne fungus that can due to more losses in yield of melon plants. Fusarium is a variable fungi in terms of morphology, symptom production, pathogenicity and wide host range. From 2013 to 2015, 120 isolates of <em>F. oxysporum,</em> <em>F. acuminatum, F. graminearum, </em> <em>F. proliferatum</em> and <em>F. solani</em> from melon, were collected from disease suspected plants from the field and defined for pathogenicity in our melon plants. Then, a sort of those were elected for phylogenetic analysis. In the beginning, the <em>Fusarium</em> isolates were classified based on morphology and identifications were authenticated based on sequence data from, EF1α and DNA, and then were used to determine whether <em>Fusarium</em> variability is relevant to geographic origin and pathogenicity. Neighbor-joining analyses datasets indicated some clades based on geographic origin but there is not even a single clades including solely of pathogens. Because of the factors affecting in pathogenicity are variable, we should be considered them in future studies. Due to the presence of FOM and some nonpathogenic isolates in almost all clades, it is obvious that <em>Fusarium oxysporum</em> f. sp. <em>melonis</em> is not monophyletic.</p>

Eff ect of gamma rays on germination, morphological and yield characters of bhendi (Abelmoschus esculentus [L.] Moench)

<p>Bhendi (Abelmoschus esculentus [L.] Moench) is the most common vegetable crop of the world. The genus Abelmoschus belongs to family Malvaceae is represented by 12 species. This investigation was carried out to the study of different doses of gamma irradiation on bhendi seeds of varieties arka anamika. Gamma irradiation treatment at 10, 20, 30, 40, 50, and 60 KR levels. The main objective of the present study is to determine the effect gamma irradiation on different morpho-agronomic characteristics. Agronomic traits were analyzed such as days of first flower, height of plants, number of fruits per plant, fruit length, seed yield per plant, fresh weight per plant, dry weight per plant, 100 seed weight. The seed pre-soaked in distilled water for 6 h before the sown in the field. The results showed that the positive shift with respect to all parameters. High doses of gamma<br />irradiation (40 and 50 KR) observed in moderate to high values.</p>

Application of somatic hybridization for the improvement of horticultural crops

<p>Somatic hybridization (SH) using protoplast fusion is a promising tool to produce symmetrical and asymmetrical polyploidy somatic hybrids in many agricultural crops. The technique of SH could facilitate conventional breeding by providing of novel lines so as to use them as elite breeding materials in conventional crosses for both scion and rootstock improvement. Further, SH can overcome those problems associated with sexual hybridization viz., sexual incompatibility, nucellar embryogenesis, and male/female sterility. Successful exploitation of SH in horticultural crops mainly comes from transfer of resistance genes for biotic and abiotic stresses from related species in several horticultural crops, <em>viz</em>., citrus, potato, brinjal, tomato, mango, avocado, banana, strawberry, pear, cherry etc. Unlike transgenic technology, SH is not affected by legal formalities and able to transfer uncloned multiple genes. However, certain boundaries and limitations of SH restricts its use over sexual hybridization but, envisage of new genomic technologies providing better insight into the plant genomes will increase the potentiality of SH in betterment of agriculture.</p>

Heterotic expression in inbreds derived from four different base populations in maize (Zea mays L.).

The concepts of combining ability and heterosis are the fundamental tools for enhancing productivity of different crops. The main objective is to study the usefulness of inbreds derived from four different base populations namely advanced generations of single cross hybrids, three way cross hybrids, hybrid mixtures and composites by analyzing the combining ability of inbreds and crosses derived and the heterosis obtained for important characters. One hundred and forty four maize inbreds derived from four different base populations namely advanced generations of single cross hybrids, three way cross hybrids, hybrid mixtures and composites were studied for their heterotic expression. The results indicated that composite and hybrid mixture base populations shall be of great use in deriving genetically divergent inbreds and single cross hybrids with significant standard heterosis suitable for commercial exploitation.

ORGANIC FERTILIZATION AND NATURAL SUBSTANCES TREATMENTS AFFECTS CHEMICAL CONSTITUENTS OF GUAR PLANTS

This experiment was conducted at the Floriculture Nursery, Faculty of Agriculture, Minia University during the two successive seasons to study the effect of compost at rates (0, 25, 2.50 and 3.75 ton/fed.) and natural substances i.e. vitamins (ascorbic acid at 50 and 100 ppm, ?-tocopherol at 10, 20 ppm and thiamine, at 25 and 50 ppm) and active yeast at 2.5 and 5 g/l. and their interaction on some chemical constituents of guar plants. The results indicated that, Addition of compost caused an increase in guaran %, Photosynthetic pigments, Total carbohydrates (%) N, P and K (%) and Protein (%). The maximum level in all previous traits was for the plants growing in the soil fertilized with the highest level of compost (3.75 t/fed.). Using the treatments of vit. C at 50 ppm followed by 5 g/l. active yeast, then vit. E at 10 ppm gave the highest values of all previous traits. The interaction between the two main factors (A×B) was significant for guaran %, photosynthetic pigments, total carbohydrates %, N and P %., The highest values were obtained from the interaction treatments of 3.75 ton/fed., compost in combination with active yeast at 5 g/l. and vit. C at 50 ppm. On the other hand, the interaction between compost, active yeast and vitamins treatments was not significant for protein content (%). We conclude that supply guar plants with compost at 3.75 ton/fed., and spraying plants with either active yeast at 5 g/l. or ascorbic acid (vit. C) at 50 ppm to improve the values of some chemical constituents under investigation condition.

Regional Polymorphism Assessment of Myanmar ‘Sein Ta Lone’ Mango (Mangifera indica Linn) from Sintgaing Township Based on Microsatellite Markers

This study was conducted to explore the genetic diversity and relationship of Sein Ta Lone mango cultivars among 20 commercial orchards in Sintgaing Township, Mandalay region. Nine microsatellite (SSR) markers were used to detect genetic polymorphism in a range from (3 to 6) alleles with (4.33) alleles per marker in average. Six out of nine microsatellite markers gave the PIC values of greater than (0.5). Among them, SSR36 held the highest PIC values of (0.691) while MiSHRS39 and MN85 possessed the least PIC values of (0.368) and (0.387) respectively. The genetic diversity was expressed as unbiased expected heterozygosity (UHe) value with an average of (0.561). The genetic relationship was revealed by (UPGMA) dendrogram in a range of (0.69 to 1.00). Based on UPGMA cluster analysis, three main clusters were classified among three different locations. This study was intended to help cultivar characterization and conservation for proper germplasm management with the estimation of genetic variation and relationship in the existing population of Sein Ta Lone mangoes in Sintgaing Township by microsatellite markers.

UTILIZATION OF CONVENTIONAL AND NON-CONVENTIONAL ORGANIC SOURCES AND INDUSTRIAL BY-PRODUCTS FOR YIELD MAXIMIZATION OF BRINJAL IN COASTAL SOIL

This study was conducted to evaluate the response of brinjal (Solanum melongena L.) with conventional, non-conventional organic source, industrial by-products combined with inorganic fertilizers. The treatments imposed were T1 – Control (100% RDF), T2-100% RDF + Municipal Solid Waste Compost @ 5 t ha-1, T3 – 100% RDF + Municipal Solid Waste Compost @ 10 t ha-1, T­4 – 100% RDF + Vermicompost @ 2.5 t ha-1, T5 – 100% RDF + Vermicompost @ 5 t ha-1, T6 – 100% RDF + Rice Husk Ash @ 5 t ha-1, T7 – 100% RDF + Rice Husk Ash @ 10 t ha-1, T8 – 100% RDF + Lignite Fly Ash @ 5 t ha-1, T9 – 100% RDF + Lignite Fly Ash @ 10 t ha-1. There were nine treatments combinations replicated thrice in CRD. The soil was sandy in texture with available nitrogen, phosphorus and potassium of 257, 10.2 and 117 kg ha-1 respectively which fell in fertility status of low. The soil classified taxonomically as Typic Udisamments. The results showed that application of 100% RDF + Vermicompost @ 5 t ha-1 (T5) significantly increased yield attributes viz., fruit length (16.6 cm), fruit girth (15.2 cm), no. of fruits plant-1 (20.67), fruit weight (45.2 g) and fruit yield plant-1 (934.2 g plant-1). This treatment registered maximum quality attributes like ascorbic acid content (4.5 mg 100 g-1), crude protein (4.81%), drymatter of fruit (70.2 g plant-1) and dry matter of plant (81.3 g plant-1). The post harvest soil available N (148mgkg-1) and P (7.15mgkg-1)status was higher due to application of 100% RDF with Vermicompst @ 5 t ha-1 (T5). The post harvest available K (75.58mgkg-1) status recorded higher in the treatment T9 which received 100% RDF with Lignite Fly Ash @ 10 t ha-1 (T9).