Isolation and screening producing growth regulator cyanobacteria strains

Cyanobacteria are photosynthetic microorganisms that have their biosynthesis capacities for secondary compounds with the high application value. They can produce a variety of bioactive compounds such as lipopeptides, fatty acids, toxins, carotenoids, vitamins and plant growth regulators which could be released into the culture medium. The present study aimed to isolate and screen cyanobacteria strains that could synthesize phytohormone, indole-3-acetic acid (IAA) from paddy soild and fresh water ecosystems (canals, river). Soil and water samples were collected from diferent provinces (Bac Giang, Thanh Hoa and Hue). Indole-3-acetic acid was extracted from the culture of isolated cyanobacteria strains and identified using the Salkowski method. As a result, total 10 strains belonging to 4 genera including Nostoc, Anabena, Geitlerinema and Planktothricoides were susscessful isolated from river, canal and rice field. The morphology of isolated taxa was characterized and monoalgal cultures were grown in BG 11 medium. In L-tryptophan-enriched growth media, all cyanobacteria strains in this research were able to biosynthesize growth regulators with IAA concentrations ranging from 9.1 to 95 µg/mL. Among the isolated cyanobacteria strains, the Planktothricoides raciborskii showed potential for the production of IAA even in the absence of tryptophan in the culture medium. Research results of the L-tryptophan concentration effect on the ability of IAA biosynthesis of this cyanobacteria strain showed that IAA concentration increased gradually and reached the highest value (118,28 ± 2,00 µg/mL) when supplementing L- tryptophan in culture medium at 900 µg/mL. The capacity of producing IAA makes these isolated cyanobacteria an appopriate cadidate for agricultural biotechnology.

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Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.44.38 ◽

2015 ◽

Vol 44 ◽

pp. 38-44 ◽

Cited By ~ 1

Author(s):

H. Sandhya ◽

Rao Srinath

Keyword(s):

Acetic Acid ◽

Growth Regulators ◽

Callus Induction ◽

Basal Medium ◽

Direct Regeneration ◽

Naphthalene Acetic Acid ◽

Stem Explants ◽

Dichlorophenoxy Acetic Acid ◽

Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

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Response of pine hypocotyl sections to growth regulators and related substances

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1975.012 ◽

2015 ◽

Vol 44 (1) ◽

pp. 123-132

Author(s):

J. Zakrzewski

Keyword(s):

Acetic Acid ◽

Growth Regulators ◽

Growth Response ◽

Extended Period ◽

Dichlorophenoxyacetic Acid ◽

Pinus Silvestris ◽

Related Substances ◽

2,4 Dichlorophenoxyacetic Acid ◽

Indole 3 Acetic Acid ◽

Synthetic Growth

Growth response of <i>Pinus silvestris</i> hypocotyl sections to some synthetic growth regulators and related substances was studied. Elongation of hypocotyl sections was stimulated by naphtaleneacetic acid, indole-3-acetic acid, in-dole-3-propionic acid, indole-3-butyric acid, 2,4-dichlorophenoxyacetic acid, indoleaoetic amide, indoleacetic nitrile and coumarin. Indole-3-acetic acid and naphtaleneacetic acid extended period of growth up to 16 and 24 hours, respectively. Growth was inhibited by kinetin, trans-cinnamic acid and 2,3,5-tri-iodobenzoic acid. No effect of gibberellic acid, tryptophan and biotin was observed.

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In vitro callus induction of gerbera from leaf explant

International Journal of Advanced Geosciences ◽

10.14419/ijag.v8i1.30000 ◽

2020 ◽

Vol 8 (1) ◽

pp. 1

Author(s):

Sadia Afrin Jui ◽

Md. Mijanur Rahman Rajib ◽

M. Mofazzal Hossain ◽

Sharmila Rani Mallik ◽

Iffat Jahan Nur ◽

...

Keyword(s):

Acetic Acid ◽

Growth Regulators ◽

Callus Induction ◽

Leaf Explants ◽

Leaf Explant ◽

Naphthalene Acetic Acid ◽

Dichlorophenoxyacetic Acid ◽

Ms Medium ◽

Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.

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Studies on Plant Growth Regulators. II. Isolation of Indole-3-acetic Acid, Phenylacetic Acid, and Several Plant Growth Inhibitors from Etiolated Seedlings of Phaseolus

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.15.159 ◽

1967 ◽

Vol 15 (2) ◽

pp. 159-163 ◽

Cited By ~ 19

Author(s):

Toshihiko Okamoto ◽

Toru Koizumi ◽

Yo Isogai

Keyword(s):

Acetic Acid ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Phenylacetic Acid ◽

Growth Inhibitors ◽

Etiolated Seedlings ◽

Indole 3 Acetic Acid ◽

Plant Growth Inhibitors

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Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues

Applied and Environmental Microbiology ◽

10.1128/aem.71.3.1169-1177.2005 ◽

2005 ◽

Vol 71 (3) ◽

pp. 1169-1177 ◽

Cited By ~ 82

Author(s):

Olivier Vandeputte ◽

Sevgi Öden ◽

Adeline Mol ◽

Danny Vereecke ◽

Koen Goethals ◽

...

Keyword(s):

Acetic Acid ◽

Acid Production ◽

Infected Plant ◽

Growth Media ◽

Acid Biosynthesis ◽

Gram Positive ◽

Acetic Acid Production ◽

Rhodococcus Fascians ◽

Wide Range ◽

Indole 3 Acetic Acid

ABSTRACT The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed.

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Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532011001200017 ◽

2011 ◽

Vol 22 (12) ◽

pp. 2355-2361 ◽

Cited By ~ 6

Author(s):

Rosa E. Prieto C. ◽

Nancy M. Cordoba C. ◽

Andres M. Montenegro J. ◽

Gloria E. González-Mariño

Keyword(s):

Acetic Acid ◽

Culture Medium ◽

Scenedesmus Obliquus ◽

Indole 3 Acetic Acid

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Silicon induces adventitious root formation in rice (Oryza sativa L.) under arsenate stress with the involvement of nitric oxide and indole-3-acetic acid

Journal of Experimental Botany ◽

10.1093/jxb/eraa488 ◽

2020 ◽

Author(s):

Durgesh Kumar Tripathi ◽

Padmaja Rai ◽

Gea Guerriero ◽

Shivesh Sharma ◽

Francisco J Corpas ◽

...

Keyword(s):

Nitric Oxide ◽

Acetic Acid ◽

Adventitious Root ◽

Root Formation ◽

Oryza Sativa L ◽

Adventitious Root Formation ◽

Growth Media ◽

Simultaneous Application ◽

Arsenate Stress ◽

Indole 3 Acetic Acid

Abstract Arsenic (As) negatively affects plant development. Using rice as a model, this study evaluates how the application of silicon (10 µM Si) can favour the formation of adventitious roots under arsenate stress (50 µM As V) as a mechanism to mitigate its negative effects. Indeed, the simultaneous application of As V and Si up-regulated the expression of genes involved in nitric oxide (NO) metabolism (OsNOA1), cell cycle progression (G2-M, OsCDKA1), auxin (IAA, indole-3-acetic acid) biosynthesis (OsYUCCA1 and OsTAA1) and transport (OsPIN1, OsPIN5 and OsPIN10) and Si uptake (OsLsi1 and OsLsi2), which accompanied adventitious root formation. Furthermore, Si triggered the expression and activity of MDHAR and DHAR involved in ascorbate recycling. The treatment with L-NAME, an inhibitor of NO generation, significantly suppressed adventitious root formation, even in the presence of Si; however, supplying NO in the growth media rescued its effects. The data obtained suggest that both NO and IAA are essential for Si-mediated adventitious root formation under As V stress. Interestingly, TIBA (a polar auxin transport inhibitor) suppressed adventitious root formation, even in the presence of Si and SNP (an NO donor), suggesting that Si is involved in a mechanism whereby a cellular signal is triggered and requires NO formation first and, then, IAA.

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