Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits

2013 ◽  
Vol 39 (6) ◽  
pp. 635-642 ◽  
Author(s):  
Flaviana Soares Rocha ◽  
Jonas Dantas Batista ◽  
Darceny Zanetta-Barbosa ◽  
Paula Dechichi
Keyword(s):  
Bone Graft ◽  
Bone Grafts ◽  
Saline Group ◽  
Histological Evaluation ◽  
Autogenous Bone ◽  
Air Exposure ◽  
Recipient Site ◽  
Storage Media ◽  
Graft Area ◽  
Autogenous Bone Grafts

The success of autogenous bone graft is related to the graft cell viability. In bone-grafting procedures, harvested grafts are often maintained in extraoral media while the recipient site is prepared. The aim of this study was to evaluate in vivo the effect of storage media over autogenous bone grafts during the transsurgical time. Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was immediately fixed in the mandibular angle (control group), and the other was maintained in air exposure (dry group), 0.9% NaCl solution (saline group), or platelet-poor plasma (PPP group) during 30 minutes and stabilized in the symmetrical location of control grafts. After 28 days, the animals were euthanized and the bone fragments were removed, demineralized, and embedded in paraffin. Histological evaluation was performed under light microscope. Empty lacunae and bone graft area quantification were carried out for the sections. The histomorphometrical analysis revealed reduction of the graft area and increase of empty lacunae in the dry group when compared with control. No significant differences were found in the number of empty lacunae or bone graft area between the saline group and its control and also between the PPP group and its control. The dry group showed more empty lacunae and less graft area than the saline and PPP groups. In accordance with the results, PPP and physiologic solution demonstrated osteocyte preservation and bone graft area maintenance, being satisfactory storage media for autogenous bone grafts during the transsurgical period.

Computerized Tomographic Evaluation of Symphyseal Donor Sites Used in the Reconstruction of the Posterior Maxilla: A Case Report of 2 Patients

2011 ◽  
Vol 37 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Jun-Beom Park
Keyword(s):  
Bone Graft ◽  
Dimensional Change ◽  
Bone Grafts ◽  
Long Term Results ◽  
Autogenous Bone ◽  
Posterior Maxilla ◽  
Donor Area ◽  
Ct Analysis ◽  
Autogenous Bone Grafts

Abstract Autogenous bone grafts are commonly used for reconstruction of the deficient maxillary alveolar ridge. Mandibular ramus, symphysis, extraction sites, and retromolar sites are the most common sources for harvesting autogenous bone grafts from intraoral regions. Several authors have used computerized tomography (CT) to evaluate the dimensional change of bone graft, but there are limited reports evaluating donor sites for autogenous bone. In this report, 2 patients were treated with autogenous bone graft from the mandibular symphysis. Postoperative CT scans were used to evaluate the radiographic change in the symphyseal area. The patients treated with autogenous bone showed uneventful healing at the donor and recipient sites and good acceptance of the procedure. At the final evaluation, CT analysis demonstrated a persisting radiolucency in the donor area, even though the defect size was reduced. Further evaluation may be needed to evaluate the progress of remineralization and long-term results.

Tissue response to poly-L-lactide acid-polyglycolic acid absorbable screws in autogenous bone grafts: a histologic morphological analysis

2004 ◽  
Vol 16 (1) ◽  
pp. 112-118 ◽  
Author(s):  
Mariza Akemi Matsumoto ◽  
Hugo Nary Filho ◽  
Luís Eduardo Marques Padovan ◽  
Roberto Yoshio Kawakami ◽  
Luís Antônio De Assis Taveira
Keyword(s):  
Morphological Analysis ◽  
Bone Grafts ◽  
Polyglycolic Acid ◽  
Tissue Response ◽  
Autogenous Bone ◽  
Autogenous Bone Grafts

scholarly journals Growth Dynamic of Allogeneic and Autogenous Bone Grafts in a Vertical Model

2018 ◽  
Vol 29 (4) ◽  
pp. 325-334 ◽  
Author(s):  
Julio Leonardo de Oliveira Lima ◽  
Daniel Isaac Sendyk ◽  
Wilson Roberto Sendyk ◽  
Cristiane Ibanhes Polo ◽  
Luciana Correa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Pure Titanium ◽  
Bone Grafts ◽  
Autogenous Bone ◽  
Growth Dynamic ◽  
Freeze Dried ◽  
Fresh Frozen ◽  
Hollow Cylinders ◽  
Autogenous Bone Grafts

Abstract Several techniques have been proposed for vertical bone regeneration, and many of them use bone autogenous and allogeneic grafts. The purpose of this study was to compare demineralised freeze-dried bone allografts (DFDBA), fresh-frozen (FF) allografts, autogenous bone grafts to find differences between volumetric and histological quantity of bone formation and vertical bone growth dynamic. A vertical tissue regeneration bone model was performed in rabbit calvarias under general anaesthesia. Four hollow cylinders of pure titanium were screwed onto external cortical bone calvarias in eight rabbits. Each one of the cylinders was randomly filled with one intervention: DFDBA, FF, autogenous bone, or left to be filled with blood clot (BC) as control. Allogeneic grafts were obtained from a ninth animal following international standardised protocols for the harvesting, processing, and cryopreservation of allografts. Autogenous graft was obtained from the host femur scraping before adapting hollow cylinders. Animals were euthanized at 13 weeks. Vertical volume was calculated after probe device measurements of the new formed tissue inside the cylinders and after titanium cylinders were removed. Histomorphometry and fluorochrome staining were used to analyse quantity and dynamic of bone formation, respectively. Results showed that DFDBA and fresh-frozen bone improved the velocity and the quantity of bone deposition in distant portions of the basal plane of grafting. Remaining material in allograft groups was more intense than in autogenous group. Both allografts can be indicated as reliable alternatives for volume gain and vertical bone augmentation.

Long-term results of mandibular reconstruction with autogenous bone grafts and oral implants after tumor resection

2008 ◽  
Vol 19 (10) ◽  
pp. 1074-1080 ◽  
Author(s):  
Matteo Chiapasco ◽  
Giacomo Colletti ◽  
Eugenio Romeo ◽  
Marco Zaniboni ◽  
Roberto Brusati
Keyword(s):  
Tumor Resection ◽  
Bone Grafts ◽  
Mandibular Reconstruction ◽  
Long Term Results ◽  
Autogenous Bone ◽  
Oral Implants ◽  
Autogenous Bone Grafts

Osteogenic Potential of Autogenous Bone Grafts Harvested with Four Different Surgical Techniques

2011 ◽  
Vol 90 (12) ◽  
pp. 1428-1433 ◽  
Author(s):  
R.J. Miron ◽  
E. Hedbom ◽  
N. Saulacic ◽  
Y. Zhang ◽  
A. Sculean ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Surgical Techniques ◽  
Bone Grafts ◽  
Osteogenic Potential ◽  
Autogenous Bone ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Osteogenic Response ◽  
Autogenous Bone Grafts

The osteogenic potential of autogenous bone grafts is superior to that of allografts and xenografts because of their ability to release osteoinductive growth factors and provide a natural osteoconductive surface for cell attachment and growth. In this in vitro study, autogenous bone particles were harvested by four commonly used techniques and compared for their ability to promote an osteogenic response. Primary osteoblasts were isolated and seeded on autogenous bone grafts prepared from the mandibles of miniature pigs with a bone mill, piezo-surgery, bone scraper, and bone drill (bone slurry). The osteoblast cultures were compared for their ability to promote cell attachment, proliferation, and differentiation. After 4 and 8 hrs, significantly higher cell numbers were associated with bone mill and bone scraper samples compared with those acquired by bone slurry and piezo-surgery. Similar patterns were consistently observed up to 5 days. Furthermore, osteoblasts seeded on bone mill and scraper samples expressed significantly elevated mRNA levels of collagen, osteocalcin, and osterix at 3 and 14 days and produced more mineralized tissue as assessed by alizarin red staining. These results suggest that the larger bone graft particles produced by bone mill and bone scraper techniques have a higher osteogenic potential than bone slurry and piezo-surgery.

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