Participation of Insulin-Like Growth Factor 1 in Articular Cartilage Remodeling

Upon cartilage damage its natural recovery takes a long time. This is due to several reasons: the absence of blood vessels and low metabolic activity. In this regard, it is relevant to study the mechanism of operation of all components involved in the remodeling of cartilage tissue. For this purpose, protein insulin-like growth factor 1 (IGF-1) was taken as an object of research, since one of its main functions is regulation of cell proliferation, which is closely related to the process of cartilage tissue repair. It is known that in recent years, scientific studies have been conducted on the effect of IGF-1 on the remodeling of cartilage tissues, but in combination with other substances that stimulate more active cell regeneration. This review presents the features of the functional properties and protein structure and its main influence on chondrocyte proliferation for further development of accelerated and effective methods of IGF-1 action on cell growth and repair. The phylogenetic analysis of IGF-1 showed the most related organism for human IGF-1 and structural differences of its protein from human, which are inextricably linked with the functional characteristics of each of the organisms. The results of phylogenetic analysis in the future will identify the object for laboratory research in this question and the search for optimal ways to accelerate the process of remodeling of cartilage joints.

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Fabrication of Scaffold-Free Engineered Cartilage Using Passaged Chondrocytes in the Presence of Insuline Like Growth Factor 1 (IGF-1)

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.149 ◽

2007 ◽

Vol 342-343 ◽

pp. 149-152 ◽

Cited By ~ 1

Author(s):

Ri Long Jin ◽

So Ra Park ◽

Jeong Hwa Son ◽

Byoung Hyun Min

Keyword(s):

Growth Factor ◽

Articular Chondrocytes ◽

Cartilage Tissue ◽

Insulin Like Growth Factor ◽

Free System ◽

Engineered Cartilage

Two passaged (P2) immature porcine articular chondrocytes were used to fabricate an engineered cartilage tissue in an in vitro scaffold-free system with or without insulin like growth factor 1 (IGF-1). This study shows the possibility of the fabrication of structurally regular neocartilage tissue using passaged chondrocytes in the scaffold-free system with insulin like growth factor-1(IGF-1).

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Comparative Insilco physiochemical and phylogenetic analysis of Insulin like growth factor 1 receptor (IGF-1R) in domestic animals

Indian Journal of Animal Research ◽

10.18805/ijar.b-3614 ◽

2018 ◽

Author(s):

P R. Sahoo ◽

G. Sahoo ◽

P. C. Behera

Keyword(s):

Phylogenetic Analysis ◽

Growth Factor ◽

Evolutionary Genetics ◽

Domestic Animals ◽

Amino Acid Sequences ◽

Insulin Like Growth Factor ◽

Multiple Sequence ◽

Lung Cancers ◽

Physiochemical Parameters ◽

Time Of Divergence

Insulin like growth factor 1receptors (IGF-1R) are the proteins which are expressed on the cell surface of almost all tissues in human as well as domestic animals with major involvement in growth, cancer, aging, production and in early embryonic development. Due to above importance, this protein needs to be characterized both in physiochemical and phylogenetically for further exploration in livestock research. In this study, the IGF1R amino acid sequences of selected domestic animals are retrieved from UniProt database and various physiochemical parameters were compared through ProtParam insilco tool. The multiple sequence alignment (MSA) and phylogenetic analysis was performed through Clustal omega and Molecular evolutionary genetics analysis (MEGA) application platform respectively. It was found that this protein is an unstable, hydrophilic in all domestic animals with amino acids varied from 1307 to 1412 in number. The phylogenetic analysis showed that highest time of divergence occurs in killer whale and rabbit, but least time of divergence occurs between goat and bovine. So this study will provide a better platform for the development of suitable anticancer therapeutics in domestic animals in nearest future as IGF-1R is implicated in several cancers, including breast, prostate, and lung cancers.

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Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2007.04.013 ◽

2008 ◽

Vol 155 (1) ◽

pp. 201-207 ◽

Cited By ~ 32

Author(s):

Buel D. Rodgers ◽

Eric H. Roalson ◽

Cullen Thompson

Keyword(s):

Phylogenetic Analysis ◽

Growth Factor ◽

Protein Gene ◽

Binding Protein ◽

Gene Families ◽

Insulin Like Growth Factor ◽

Related Protein ◽

Factor Binding

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Effects of Artesunate on the Expressions of Insulin-Like Growth Factor-1, Osteopontin and C-Telopeptides of Type II Collagen in a Rat Model of Osteoarthritis

Pharmacology ◽

10.1159/000479160 ◽

2017 ◽

Vol 101 (1-2) ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Zhe Bai ◽

Xiao-Hui Guo ◽

Chi Tang ◽

Si-Tong Yue ◽

Long Shi ◽

...

Keyword(s):

Growth Factor ◽

Type Ii Collagen ◽

Cartilage Tissue ◽

Enzyme Linked Immunosorbent Assay ◽

Insulin Like Growth Factor ◽

Type Ii ◽

Pathological Changes ◽

Model Group ◽

Mankin Score ◽

And Cartilage

Objective: The study aims to explore the effects of artesunate on insulin-like growth factor-1 (IGF-1), Osteopontin (OPN), and C-telopeptides of type II collagen (CTX-II) in serum, synovial fluid (SF), and cartilage tissues of rats with osteoarthritis (OA). Methods: OA models were established. Normal model, artesunate, and Viatril-S groups (20 rats respectively) were set. Enzyme-linked immunosorbent assay, IHC staining, and quantitative real-time polymerase chain reaction were conducted to calculate IGF-1, OPN, and CTX-II levels in serum, SF, and cartilage tissues of rats. The pathological changes in cartilage tissues were evaluated with Mankin score and Hematoxylin-Eosin staining. Results: Compared with the normal group, the model group showed increased IGF-1 level; decreased OPN, CTX-II levels in the serum and SF; and contrary results were seen in the cartilage tissues. A gradual ascending IGF-1 level and descending OPN and CTX-II levels existed in the serum and SF in the artesunate and Viatril-S groups after 2 weeks. The model group showed the most obvious pathological changes and highest Mankin score compared with the other groups. Higher IGF-1 level and lower OPN, CTX-II levels were exhibited in the cartilage tissue in the artesunate and Viatril-S groups but not in the model group. Conclusion: Artesunate and Viatril-S inhibit OA development by elevating IGF-1 level and reducing OPN and CTX-II levels.

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