scholarly journals Evaluation of TB-LAMP assay for the diagnosis of pulmonary tuberculosis in Lusaka, Zambia

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Solo ES ◽  
Mbulo G ◽  
Shibemba AL ◽  
Lungu PS ◽  
Nsama D ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Lamp Assay

scholarly journals 1633. Improving the diagnosis of extra-pulmonary tuberculosis (EPTB): experience from north India

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S808-S808
Author(s):  
Anchal Sharma ◽  
Kusum Sharma ◽  
Manish Modi ◽  
Aman Sharma
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Rural Setting ◽  
Tuberculosis Complex ◽  
Extra Pulmonary Tuberculosis ◽  
Resource Poor ◽  
Extra Pulmonary

Abstract Background Rapid and accurate diagnosis of extra-pulmonary tuberculosis (EPTB) is imperative for early treatment and better patient outcome. Loop-mediated Isothermal Amplification (LAMP) is a promising nucleic-acid amplification assay. LAMP assay could be carried out in simple water bath under isothermal conditions in 60 minutes, and can be performed in any laboratory even in rural setting in resource poor endemic countries. We evaluated LAMP assay using two different target regions LAMP primers specific for Mycobacterium tuberculosis complex for the diagnosis of EPTB. Methods LAMP assay using 6 primers (each for IS6110 and IS1081) specific for Mycobacterium tuberculosis complex were performed on patients suspected of EPTB on various EPTB samples(CSF, Synovial fluid, Lymaphnode and tissue biopsies and various other samples) of 150 patients (50 confirmed, 100 suspected) Clinically suspected of EPTB and 100 non-TB control subjects. Results Overall LAMP test (using any of the two targets) had sensitivity and specificity of 96% and 100% for confirmed (50 culture positive) EPTB cases. In 100 clinically suspected but unconfirmed EPTB cases, LAMP was positive in 87 out of 100 cases (87%). Sensitivity of IS6110 LAMP, 1S1081 LAMP and IS6110 PCR for clinically suspected cases was 78 (78%), 84 (84%) and 70 (70%), respectively. In total 150 EPTB patients, the overall sensitivity of microscopy, culture, IS6110 PCR, IS6110 LAMP, 1081 LAMP and the LAMP test (if any of the two targets were used) were 4%, 33.3%, 74.6%, 82.66%, 87% and 92%, respectively. Specificity of all the tests was 100%. There were 8 cases which were missed by IS6110 LAMP and 2 cases by 1081 LAMP. Conclusion LAMP assay using two targets is a promising technique for rapid diagnosis of EPTB in 60 minutes especially in a resource poor setting who are still battling with this deadly disease. Disclosures All Authors: No reported disclosures

Evaluation of the TB-LAMP assay for the rapid diagnosis of pulmonary tuberculosis in Northern India

2017 ◽  
Vol 21 (10) ◽  
pp. 1150-1153 ◽  
Author(s):  
R. Yadav ◽  
N. Sharma ◽  
R. Khaneja ◽  
P. Agarwal ◽  
A. Kanga ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Lamp Assay ◽  
Rapid Diagnosis ◽  
Northern India

Evaluation of improved IS 6110 LAMP assay for diagnosis of pulmonary and extra pulmonary tuberculosis

2017 ◽  
Vol 139 ◽  
pp. 87-91 ◽  
Author(s):  
Deepali Joon ◽  
Manoj Nimesh ◽  
Mandira Varma-Basil ◽  
Daman Saluja
Keyword(s):  
Pulmonary Tuberculosis ◽  
Lamp Assay ◽  
Extra Pulmonary Tuberculosis ◽  
Extra Pulmonary

Recent Advances in the Treatment of Pulmonary Tuberculosis

1950 ◽  
Vol 34 (5) ◽  
pp. 1363-1380
Author(s):  
Theodore L. Badger ◽  
William E. Patton
Keyword(s):  
Pulmonary Tuberculosis ◽  
Recent Advances

Abstract #542 An Unusual Initial Presentation of Active Pulmonary Tuberculosis: Severe Hypercalcemia with Altered Mental Status and Renal Failure

2018 ◽  
Vol 24 ◽  
pp. 130
Author(s):  
Matthew Mandell ◽  
Jennifer Velasco ◽  
Armand Krikorian ◽  
Anupama Ananth ◽  
Joumana Chaiban ◽  
...  
Keyword(s):  
Renal Failure ◽  
Pulmonary Tuberculosis ◽  
Mental Status ◽  
Altered Mental Status ◽  
Initial Presentation ◽  
Active Pulmonary Tuberculosis ◽  
Severe Hypercalcemia
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